We used deep observations collected with Advanced Camera for Surveys (ACS) at Hubble Space Telescope (HST) to derive the fraction of binary systems in a sample of 13 low-density Galactic globular clusters. By analysing the colour distribution of main-sequence stars we derived the minimum fraction of binary systems required to reproduce the observed colour–magnitude diagram morphologies. We found that all the analysed globular clusters contain a minimum binary fraction larger than 6 per cent within the core radius. The estimated global fractions of binary systems range from 10 to 50 per cent depending on the cluster. A dependence of the relative fraction of binary systems on the cluster age has been detected, suggesting that the binary disruption process within the cluster core is active and can significantly reduce the binary content in time.