This paper presents the development and evaluation of short-term traffic prediction models using unidirectional and bidirectional deep learning long short-term memory (LSTM) neural networks. The unidirectional LSTM (Uni-LSTM) model provides high performance through its ability to recognize longer sequences of traffic time series data. In this work, Uni-LSTM is extended to bidirectional LSTM (BiLSTM) networks which train the input data twice through forward and backward directions. The paper presents a comparative evaluation of the two models for short-term speed and traffic flow prediction using a common dataset of field observations collected from multiple freeways in Australia. The results showed BiLSTM performed better for variable prediction horizons for both speed and flow. Stacked and mixed Uni-LSTM and BiLSTM models were also investigated for 15-minute prediction horizons resulting in improved accuracy when using 4-layer BiLSTM networks. The optimized 4-layer BiLSTM model was then calibrated and validated for multiple prediction horizons using data from three different freeways. The validation results showed a high degree of prediction accuracy exceeding 90% for speeds up to 60-minute prediction horizons. For flow, the model achieved accuracies above 90% for 5- and 10-minute prediction horizons and more than 80% accuracy for 15- and 30-minute prediction horizons. These findings extend the set of AI models available for road operators and provide them with confidence in applying robust models that have been tested and evaluated on different freeways in Australia.