Objective To investigate the role of transient receptor potential melatonin 2 （TRPM2） ion channels in microglia and astrocytes in temporal lobe epilepsy （TLE） -associated neuroinflammation. Methods Rats were randomly divided into the control group and epilepsy group. Seizure models were induced by lithium chloride-pilocarpine in the epilepsy group， and those in the control group were injected with the same dose of saline. The TLE rats were randomly divided into 7 subgroups according to the observation time after model establishment： acute phase （3 h group， 6 h group， 1 d group， 2 d group）， latent phase （14 d group） and chronic phase （30 d group， 90 d group） subgroups （n = 5）. The expression level of TRPM2 in the hippocampus of TLE rats at different stages were detected， and the cellular localization of TRPM2 in the brain of TLE rats was investigated. BV2 and C8 cell lines were induced by hydrogen peroxide （H2O2）. The levels of IL1-β， IL-6 and TNF-α released by BV2 and C8 cells were observed by ELISA. The leve