-
Surviving in the brine [Elektronski vir] : a multi-omics approach for understanding the physiology of the halophile fungus Aspergillus sydowii at saturated NaCl concentrationJiménez-Gómez, Irina ...Although various studies have investigated osmoadaptations of halophilic fungi to saline conditions, only few analyzed the fungal mechanisms occurring at saturated NaCl concentrations. Halophilic ... Aspergillus sydowii is a model organism for the study of molecular adaptations of filamentous fungi to hyperosmolarity. For the first time a multi-omics approach (i.e., transcriptomics and metabolomics) was used to compare A. sydowii at saturated concentration (5.13 M NaCl) to optimal salinity (1 M NaCl). Analysis revealed 1,842 genes differentially expressed of which 704 were overexpressed. Most differentially expressed genes were involved in metabolism and signal transduction. A gene ontology multi-scale network showed that ATP binding constituted the main network node with direct interactions to phosphorelay signal transduction, polysaccharide metabolism, and transferase activity. Free amino acids significantly decreased and amino acid metabolism was reprogrammed at 5.13 M NaCl. mRNA transcriptional analysis revealed upregulation of genes involved in methionine and cysteine biosynthesis at extreme water deprivation by NaCl. No modifications of membrane fatty acid composition occurred. Upregulated genes were involved in high-osmolarity glycerol signal transduction pathways, biosynthesis of β-1,3-glucans, and cross-membrane ion transporters. Downregulated genes were related to the synthesis of chitin, mannose, cell wall proteins, starvation, pheromone synthesis, and cell cycle. Non-coding RNAs represented the 20% of the total transcripts with 7% classified as long non-coding RNAs (lncRNAs). The 42% and 69% of the total lncRNAs and RNAs encoding transcription factors, respectively, were differentially expressed. A network analysis showed that differentially expressed lncRNAs and RNAs coding transcriptional factors were mainly related to the regulation of metabolic processes, protein phosphorylation, protein kinase activity, and plasma membrane composition. Metabolomic analyses revealed more complex and unknown metabolites at saturated NaCl concentration than at optimal salinity. This study is the first attempt to unravel the molecular ecology of an ascomycetous fungus at extreme water deprivation by NaCl (5.13 M). This work also represents a pioneer study to investigate the importance of lncRNAs and transcriptional factors in the transcriptomic response to high NaCl stress in halophilic fungi.Vir: Frontiers in microbiology [Elektronski vir]. - ISSN 1664-302X (Vol. 13, May 2022, str. 1-24)Vrsta gradiva - e-članekLeto - 2022Jezik - angleškiCOBISS.SI-ID - 107812355
Avtor
Jiménez-Gómez, Irina |
Valdés-Muñoz, Gisell |
Moreno-Ulloa, Aldo |
Pérez-Llano, Yordanis |
Moreno-Perlín, Tonatiuh |
Silva-Jiménez, Hortencia |
Barreto-Curiel, Fernando |
Rayo Sánchez-Carbente, María del |
Folch-Mallol, Jorge Luis |
Gunde-Cimerman, Nina |
Lago-Lestón, Asunción |
Batista-García, Ramón Alberto
Teme
halofilne glive |
ekstremofilne glive |
transkriptomika |
metabolomika |
nasičena raztopina NaCl |
pomanjkanje vode |
nizka aktivnost vode |
solni stres |
halophilic fungus |
extremophilic fungi |
transcriptomics |
metabolomics |
saturated NaCl solution |
water deprivation |
low water activity |
salt stress
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|---|
Jiménez-Gómez, Irina | |
Valdés-Muñoz, Gisell | |
Moreno-Ulloa, Aldo | |
Pérez-Llano, Yordanis | |
Moreno-Perlín, Tonatiuh | |
Silva-Jiménez, Hortencia | |
Barreto-Curiel, Fernando | |
Rayo Sánchez-Carbente, María del | |
Folch-Mallol, Jorge Luis | |
Gunde-Cimerman, Nina | 05935 |
Lago-Lestón, Asunción | |
Batista-García, Ramón Alberto |
Izberite prevzemno mesto:
Prevzem gradiva po pošti
Obvestilo
Gesla v Splošnem geslovniku COBISS
Izbira mesta prevzema
Mesto prevzema | Status gradiva | Rezervacija |
---|
Prosimo, počakajte trenutek.