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  • Extracellular cystatin F is internalised by cytotoxic T lymphocytes and decreases their cytotoxicity [Elektronski vir]
    Prunk, Mateja, farmacevtka ...
    Cystatin F is a protein inhibitor of cysteine cathepsins, peptidases involved in the activation of the effector molecules of the perforin/granzyme pathway. Cystatin F was previously shown to regulate ... natural killer cell cytotoxicity. Here, we show that extracellular cystatin F has a role in regulating the killing efficiency of cytotoxic T lymphocytes (CTLs). Extracellular cystatin F was internalised into TALL-104 cells, a cytotoxic T cell line, and decreased their cathepsin C and H activity. Correspondingly, granzyme A and B activity was also decreased and, most importantly, the killing efficiency of TALL-104 cells as well as primary human CTLs was reduced. The N-terminally truncated form of cystatin F, which can directly inhibit cathepsin C (unlike the full-length form), was more effective than the full-length inhibitor. Furthermore, cystatin F decreased cathepsin L activity, which, however, did not affect perforin processing. Cystatin F derived from K-562 target cells could also decrease the cytotoxicity of TALL-104 cells. These results clearly show that, by inhibiting cysteine cathepsin proteolytic activity, extracellular cystatin F can decrease the cytotoxicity of CTLs and thus compromise their function.
    Vir: Cancers [Elektronski vir]. - ISSN 2072-6694 (Vol. 12, iss. 12, Dec. 2020, str. 1-14)
    Vrsta gradiva - e-članek
    Leto - 2020
    Jezik - angleški
    COBISS.SI-ID - 43955203

    Povezava(-e):

    https://www.mdpi.com/2072-6694/12/12/3660
    Repozitorij Univerze v Ljubljani – RUL
    DOI

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