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  • THP-1 cells and pro-inflammatory cytokine productionTHP-1 Cells and pro-inflammatory cytokine production [Elektronski vir] : an in vitro tool for functional characterization of NOD1/NOD2 antagonists
    Jakopin, Žiga ; Corsini, Emanuela, pharmacist
    THP-1 cells express high levels of native functional nucleotide-binding oligomerization domain 1 (NOD1), NOD2, and Toll-like receptor 4 (TLR4) receptors, and have often been used for investigating ... the immunomodulatory effects of small molecules. We postulated that they would represent an ideal cell-based model for our study, the aim of which was to develop a new in vitro tool for functional characterization of NOD antagonists. NOD antagonists were initially screened for their effect on NOD agonist-induced interleukin-8 (IL-8) release. Next, we examined the extent to which the selected NOD antagonists block the NOD-TLR4 synergistic crosstalk by measuring the effect of NOD antagonism on tumor necrosis factoralpha - (TNF-alpha) secretion from doubly activated THP- 1 cells. Overall, the results obtained indicate that pro-inflammatory cytokine secretion from THP-1 provides a valuable, simple and reproducible in vitro tool for functional characterization of NOD antagonists.
    Vir: International journal of molecular sciences [Elektronski vir]. - ISSN 1422-0067 (Vol. 20, no. 17, 2019, str. 1-11)
    Vrsta gradiva - e-članek
    Leto - 2019
    Jezik - angleški
    COBISS.SI-ID - 4770417

    Povezava(-e):

    https://www.mdpi.com/1422-0067/20/17/4265
    Repozitorij Univerze v Ljubljani – RUL
    DOI

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