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MF, Centralna medicinska knjižnica, Ljubljana (CMK)
Poletni urnik (1.7. do 31.8.2024): ponedeljek do četrtek: 9.00-15.00, petek: 9.00-13.00.
26.7., 15.8. in 16.8. 2024 bo knjižnica ZAPRTA.
  • Platelet activation and blood extracellular vesicles : the influence of venepuncture and short blood storage
    Marić, Ivica ...
    Extracellular vesicles (EVs) as membrane-bound particles released by various cells are potential tools for diagnosis and treatment. Blood cells, particularly platelets, are the source of circulating ... EVs. Material: EVs were enriched with gradient ultracentrifugation and measured by nanoparticle tracking assay. A flow cytometric multiplex assay was used for cellular source determination. Activation of platelets was measured as a percentage of CD62p+/CD61+ platelets and correlated with the concentration and size of released EVs. Results: In general there was no statistically significant correlation between EVs` concentration and degree of platelet activation. EVs from different cellular sources were detected. Comparing different needle thicknesses, there was a decrease in the EVs concentration for the 16G needle versus the 21G needle, but no difference was observed for EVs` size and phenotype or platelets activation. During blood storage, platelet activation increased, but there was no effect on the EVs` concentration, size, or phenotype. Conclusions: Preanalytical factors like needle thickness and storage time can affect the MVs' properties. Activation of platelets during blood collection or blood storage occurs; however, it is difficult to determine its effect on the physiological properties of EVs since the mechanisms of EVs` biogenesis and especially clearness are not precisely known.
    Vir: Blood cells, molecules & diseases. - ISSN 1079-9796 (Vol. 106, iss. [article no.] 102842, 2024, str. 1-7)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 2024
    Jezik - angleški
    COBISS.SI-ID - 192381699

    Povezava(-e):

    https://www.sciencedirect.com/science/article/pii/S1079979624000202?via%3Dihub
    Repozitorij Univerze v Ljubljani – RUL
    DOI

vir: Blood cells, molecules & diseases. - ISSN 1079-9796 (Vol. 106, iss. [article no.] 102842, 2024, str. 1-7)

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