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  • Determination of free fatty acids and beta-hydroxybutyrate in blood serum with automatic analyser = Določanje vsebnosti prostih maščobnih kislin in beta-hidroksibutirata v krvnem serumu z avtomatskim analizatorjem
    Nemec, Marija ; Klinkon, Martina
    Determination of free fatty acids (FFA) and beta-hydroxybutyrate (BHB) was performed on automatic biochemical analyser Cobas Mira and repeatability, reproducibility and accuracy of measurements were ... determined. The repeatability was good at normal and high level of FFA in blood serum (CV % 3.25 and 0.86 %), and a bit worse, but still acceptable at low levels (CV % 4.55). The repeatability for BHB determination was good at low (CV % 2.47), normal (CV % 1.36) and high levels (CV % 0.65%). Reproducibility was examined with two control serum samples at normal and high levels and was satisfactory for both analyses. The accuracy of measurements was determined by adding different concentrations of standard solutions to blood serum samples. Before estimating the regression between measured and added concentrations, the endogenous level of FFA and BHB were subtracted. The results were good. In concluiosn the introduction of new analytical methods expanded the possibilityof early detection of subclinical and clinical metabolic diseases as well as the extent of the metabolic profile in ruminants.
    Vir: Research reports [of the] Veterinary Faculty = Zbornik Veterinarske fakultete. - ISSN 1408-8495 (Vol. 36, no. 2, 1999, str. 205-210)
    Vrsta gradiva - članek, sestavni del
    Leto - 1999
    Jezik - angleški
    COBISS.SI-ID - 120724480

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