Bu çalışmada, AB tipi diblok (mPEG-b-PCL) ve BAB tipi triblok (PCL-b-PEG-b-PCL) kopolimerler sırasıyla makrobaşlatıcı olarak mPEG (monometoksi poli(etilen glikol)) ve PEG (poli etilen glikol) ...kullanılarak ve katalizör olarak kalay oktanoat (Sn(Oct)2) kullanılarak ε-kaprolakton (ε-CL)’nun halka açılması polimerizasyonu ile sentezlendi. Blok kopolimer içerisinde, gümüş nitrat (AgNO3) metal tuzlarının indirgenmesiyle gümüş nanoparçacıkları içeren Ag/PEG-b-PCL ve Ag/PCL-b-PEG-b-PCL kolloidal çözeltileri üretilmiştir. mPEG-b-PCL ve PCL-b-PEG-b-PCL blok kopolimerlerinin karakterizasyonu, GPC, FTIR ve 1HNMR teknikleri kullanılarak yapılmıştır. Hibrit yapı içerisindeki gümüş nanoparçacık formasyonu, yüzey plazmon rezonans (SPR) dalga boyu değişiminden ve floresans emisyon spektrumundan gözlenmiştir. İlaç taşıyıcı sistemlerde model ilaç olarak kullanılan metilen mavisinin, polimerde gümüş nanoparçacıkların varlığında, löko-metilene indirgenme hızı araştırılmıştır.
This work refers to the synthesis and characterization of poly (3-hydroxybutyrate)-b-oligo (2-ethyl oxazoline) (oligoEtOx). Cationic ring-opening polymerization of 2-ethyl oxazoline yielded poly ...(2-ethyl oxazoline) (oligoEtOx) with a hydroxyl end. Carboxylic acid-terminated PHB was reacted with oligoEtOx via dicyclohexylcarbodiimide chemistry to obtain PHB-b-oligoEtOx conjugates. The obtained PHB-b-oligoEtOx conjugates were successfully characterized by
H- and
C NMR, FTIR, DSC, and size exclusion chromatography. PHB-b-oligoEtOx conjugates can be promising biologic active materials.
Infection is a serious complication after nasal packing that otolaryngologists seek to avoid. The aim of this study is to investigate the use of silver (Ag) nanoparticle, which serves as ...antimicrobial agents, with nasal tampons. The study design is an experimental animal model and the setting is tertiary referral center. Twenty-four rats were randomized into the following four groups: (1) control group (
n
= 6); (2) silicone nasal splint (SNS) group (
n
= 6); (3) polypropylene-grafted polyethylene glycol (PP-g-PEG) amphiphilic graft copolymer-coated SNS group (
n
= 6); and (4) Ag nanoparticle-embedded PP-g-PEG (Ag-PP-g-PEG) amphiphilic graft copolymer-coated SNS group (
n
= 6). These tampons were applied to rats for 48 h, after which they were removed in a sterile manner, and the rats were sacrificed. The nasal septa of the rats were excised, and assessments of tissue changes in the nasal mucosa were compared among the groups. The removed tampons were microbiologically examined, and quantitative analyses were made. When the groups were compared microbiologically, there were no significant differences in bacterial colonization rates of coagulase-negative
Staphylococcus
spp. among the three groups (
p
= 0.519), but there was a statistically significant difference among bacterial colonization rates of
Heamophilus parainfluenzae
and
Corynebacterium
spp. (
p
= 0.018,
p
= 0.004). We found that
H. parainfluenzae
grew less robustly in the Ag-PP-g-PEG than the PP-g-PEG group (
p
= 0.017). However, we found no significant difference between the Ag-PP-g-PEG and SNS groups, or between the SNS and PP-g-PEG groups. The growth of
Corynebacterium
spp. did not differ significantly between the Ag-PP-g-PEG and SNS groups (
p
= 1.000). When Group 4 was compared with Group 2, the former showed less inflammation. Compared with other tampons, Ag-PP-g-PEG amphiphilic graft copolymer-coated silicone nasal tampons caused less microbiological colonization and inflammation. Therefore, the use of these tampons may prevent secondary infections and reduce the risk of developing complications by minimizing tissue damage.
