This study aims identify E. coli and its β-lactamase encoding genes, S. aureus and its enterotoxin genes isolated from milk and Kariesh cheese. Moreover, we evaluated the antibacterial effect of ...lactoferrin against these pathogenic bacteria. Sixty samples in total (30 each of raw milk and Kariesh cheese) were collected from various retail-markets in Kafrel-Sheikh Governorate. The percentage of E. coli isolates found in raw milk and Kariesh cheese reached 43.3% and 36.6%, respectively, while S. aureus isolates were recorded at 50% and 23.3% (from raw milk and Kariesh cheese). Twenty-four strains of E. coli were serogrouped, of which 3 strains out of 24 were O17, O91 and O159, 6 strains were O127 and 9 strains were O26. PCR analysis for β-lactamase encoding genes in E. coli indicated that all eight isolates were 100% positive for blaTEM and blaSHV genes while 5 (62.5%) S. aureus isolates were positive for enterotoxin production. Five (62.5%) isolates produced Seb, 2(25%) produced Sec while the Sea gene was not detected in S. aureus isolates. The results indicate that lactoferrin 5% had a significant inhibitory effect on S. aureus and E. coli when they were inoculated into Kariesh cheese. The findings show that dairies didn't take enough hygiene precautions, and we advise following stringent hygiene procedures when dairy products are milked, processed and distributed. To control the growth of E. coli and S. aureus in dairy products,lactoferrin is thought to be a potential strategy.
In this study, we evaluated the inflammatory responses induced by aluminum silicate (AS) cytotoxicity in rat lungs. The prophylactic effect of propolis extract was evaluated in 60 adult male albino ...rats. The rats were divided into six groups: (1) a normal, healthy control group; (2) a normal group fed with 200 mL of propolis extract/Kg; (3) a low-dose positive control group injected with 5 mg/kg of AS; (4) a treated group given propolis and a low dose of AS; (5) a high-dose positive control group injected with 20 mg/kg of AS; and (6) a treated group given propolis with a high-dose of AS. At the end of the two-month experiment, the rats' lungs were removed. For each pair of lungs, one portion was subjected to biochemical analysis and the other underwent hematoxylin and eosin (H&E) staining in order to study its histology. The rats that received AS doses displayed significant disorders in their antioxidant contents as well as in their enzymatic activities and their histopathological structures revealed severe damage to their lung tissues. Upon the rats being treated with propolis, the enzymatic and antioxidant contents improved and partial improvements in the lung structures appeared, including minimized congestion, a reduced hemorrhage of blood vessels and preserved bronchioles, alveolar ducts, and alveoli. The prophylactic effectiveness of propolis extract on the cytotoxicity of AS, owing to the antioxidant properties of propolis, were studied.
Juvenile idiopathic arthritis (JIA) is the most common chronic rheumatic disease in children and is a leading cause of acquired disability in the pediatric age. We aimed to detect subclinical ...synovitis in children with oligoarticular JIA using high frequency power Doppler ultrasonography (US) and correlate US scores with clinical disease severity.
High frequency power Doppler ultrasonography was done for both knees and ankles of 20 oligoarticular JIA patients. Assessment of clinical disease severity by articular index scores and functional assessment by the modified juvenile arthritis functionality scale (JAFS) were calculated for all patients.
We found 24 clinically active knees and 20 clinically active ankles. On comparison between clinical and ultrasonography activity, we found 13 clinically inactive joints, but they were active joints by ultrasonography. There was a highly significant difference (p<0.001) between clinical and US activity as regards the number of affected joints. There was a highly significant (p<0.01) positive correlation between clinical disease severity scores and all US scores except clinical score of range of motion (ROM); that did not show a significant positive correlation with US score of effusion (p>0.05).
High frequency power Doppler US may be useful in detecting subclinical synovitis of joints in JIA patients with clinically defined inactive joints. Also, it can indicate the degree of disease severity in JIA patients. So, high frequency power Doppler US can be used in standard clinical practice for monitoring JIA patients for better assessment and management of the disease.
Background: Diabetes mellitus is best described as a condition that is characterized by postprandial hyperglycemia that has two types; diabetes type 1 and type 2. Many patients with type 2 diabetes ...can be asymptomatic. There are many novel biomarkers for the detection of diabetes type 2, such as FABP1, which is investigated as a marker to detect patients with diabetes type 2. Aim: The aim of the study was to investigate FABP1 in Patients with diabetes type 2 and to find the correlations between FABP1 and fasting insulin in those patients. Methods: Samples were collected from 99 diabetic patients and 85 samples of healthy participants as the control group. All participants were subjected to liver enzymes (ALT and AST) lipid profile (triglycerides, HDL, LDL, and cholesterol), T-billirbun, Albumin, Alp, AFP, BMI, S-creatinine, Hb, Fatty acids, F. Insulin, CA19.9, HbA1c, and Fbs that were done by an autoanalyzer. The serum level of fatty acid-binding protein 1 was measured by SunRed human FABP1 Elisa kits. Results: Data was represented as mean ± standard deviation or median with statistically significant values of ALT, AST, ALP, PT, and INR (at P< 0.05). Findings revealed a significant positive correlation between our marker FABP1 and TG, cholesterol, LDL, Fasting insulin, and CA19.9, Conclusion: FABP1 can be used as a novel marker to detect patients with diabetes type 2.
PDF
