Phytocosmetic is an important aspect of traditional medicine in several cultures. Researchers are now focusing to find new and effective ingredients of natural origin. Propolis is a natural beehive ...product extensively used in traditional medicine. We aimed in the present study to investigate the potential use of propolis as an aesthetic and phytotherapeutic constituent in phytocosmetics. Propolis was extracted using 80% ethanol. Total phenolic and flavonoid contents were determined calorimetrically. Free radical scavenging ability and reducing capacity were evaluated using four assays and expressed as IC50 values. Antibacterial activity was evaluated by the determination of minimum inhibitory concentration (MIC) on 11 Gram-positive and Gram-negative bacteria. The wound healing activity of 30% ethanolic extract and propolis ointment was studied using excision wounds in the anterio-dorsal side of the rats. The phenolic acid composition of the tested propolis was investigated using UFLC/MS-MS analysis. The tested propolis was rich in phenolic and flavonoid content and demonstrated an interesting antibacterial and antioxidant activity. Wounds treated with propolis appear to display a lesser degree of inflammation. Chemical analysis led to the identification of 11 phenolics. Among them, five are considered as main compounds: Chlorogenic acid (48.79 ± 5.01 ng/mL), Gallic acid (44.25 ± 6.40 ng/mL), Rutin (21.12 ± 3.57 ng/mL), Caffeic acid (28.19 ± 4.95 ng/mL), and trans-cinnamic acid (20.10 ± 6.51 ng/mL). Our results indicated that propolis can not only be used as a cosmetic ingredient but also be used as a preventative and curative constituent, which might be used as a barrier when applied externally on infected and non-infected skin.
The present study reports a methodology for the synthesis of symmetrical and unsymmetrical thiourea derivatives 1-9, which have been prepared in good overall yields from condensation reactions ...between phenylisothiocyanate and different primary amines. The structures of all products were confirmed by the spectroscopic analysis (UV, IR, and NMR). These derivatives were tested for anticoagulant activity using PT and APTT reagents, and those reagents are considered also a controller, the results showed that the theN-aryl thiourea derivatives have very high anticoagulant activity. Theoretical calculations based on DFT/B3LYP method have been performed in order to get insights into the molecular geometry and chemical reactivity of the studied compounds.
The current study first describes the extraction of phytoantioxidant polyphenols from Carob byproducts (pods) using maceration and heating-assisted extraction as traditional methods and ...ultrasonic-assisted extraction (UAE) as an innovative method to determine the most efficient extraction process in terms of four targeted responses: total phenolic content (TPC), antioxidant activities (TAC and DPPH), and photoprotective properties as measured by the sun protection factor (SPF). Second, we used response surface methodology (RSM) with a central composite rotatable design (CCDR) approach to investigate the influence of process variables (extraction time, extraction temperature, and solvent concentration) on UAE, which was found to be the most effective extraction technique in our study. Carob byproduct extracts had a TPC ranging from 6.21 to 21.92 mg GAE/g dw, a TAC ranging from 22.00 to 49.30 mg AAE/g dw, DPPH scavenging activity ranging from 56.35 to 90.50%, and SPF values ranging from 8.62 to 22.37. The optimal UAE conditions for maximum TPC, TAC, DPPH, and SPF responses were determined to be 38.90% ethanol, 53.90 °C, and 50.92 min. Using Carob as a source for sustainable and bioactive products in conjunction with optimized UAE is a promising contribution to the cosmetic industry that will help to strengthen the concept of environmentally-friendly "green chemistry". Given that Carob pulp or seeds are considered food byproducts, the research presented here encourages the use of these agri-food waste materials in cosmetics.
The benefits of dinitrones (N,Nand#39;-Diphenyl-2,5-thenylenedinitrone, N,Nand#39;-Dimethyl-1,4-phenylenedinitrone and N,Nand#39;-Dibenzyl-1,4-phenylenedinitrone) in the synthesis were chosen as ...simple process for synthesis of the purified dihydroxamic acid compounds in a shorter time with a good yield and high purity without need to purification. The most stable conformers of the synthesized compounds and their electronic properties have been determined using DFT/B3LYP calculations. Antimicrobial activity assessment of dihydroxamic acids showed their good efficiency.
The isoquinoline nucleus is the fundamental structure of many natural and synthetic biologically active substances. This scaffold is found in a variety of compounds, including antihypertensive ...agents, anesthetics, and vasodilators. In the present work, the synthesis and antibacterial activity of novel 3-amino-1,2-dihydroisoquinoline derivatives have been reported. Theoretical calculations were performed to get insights into the molecular geometry and electronic properties of the synthesized compounds. The antibacterial activity of all compounds has been evaluated against five different bacteria strains and found potentially effective antibacterial agents. Among them, compound
3a
was found to exhibit MIC value less than 0.25 mg/mL against
Staphylococcus aureus
, while compound
3b
exhibits MIC value less than 0.25 mg/mL for both strains
Staphylococcus aureus
and
Klebsiella pneumonia
. The docking study revealed a good affinity toward the DNA gyrase enzyme. Furthermore, in silico ADME calculations revealed that all compounds had favorable pharmacokinetic properties.
Propolis is a natural resinous material produced by honeybees. The biological activity and phenolic profile of propolis were largely studied all over the world. However, only a few investigations ...have been carried out on Algerian and Turkish propolis. The aim of the present study was to compare the phenolic content, antioxidant, and antibacterial activity of propolis samples collected from different localities of Algeria and Turkey.
Propolis extracts were performed using maceration in ethanol 80%. Total phenolic and flavonoid contents were determined. Antioxidant and antibacterial activities were evaluated using FRAP assay and the MIC was determined against four bacterial strains (S. aureus ATCC 25923, E. coli ATCC 25922, P. aeruginosa ATCC 27853 and K. pneumonia).
TP varied from 19.51 ± 0.86 to 219.66 ± 1.23 mg GAE/g. Whereas, TF varied from 5.27± 0.07 to 74.57 ± 1.03 QE/g. All samples showed good ferric reducing antioxidant power ranging from 267.30 ± 4.77 to 2387.30 ± 44.15 μmol Trolox eq./g. All Algerian propolis samples displayed a more pronounced activity against S. aureus ATCC 25923 with MIC values ranging from 0,04 ± 0.00 mg/mL to 0.30±0.06 mg/Ml, with an activity 30 times more powerful than Anatolian propolis. While, Anatolian propolis samples were most active against P. aeruginosa ATCC 27853 with MIC values ranging from 0.20±0.00 mg/mL to 0.60±0.00 mg/Ml, with an activity 5 to 10 times more powerful than Algerian propolis.
Algerian and Anatolian propolis possessed considerable phenolic and flavonoids contents. In addition, they exhibited interesting antioxidant and antibacterial activities. Our findings suggest that both propolis could be useful in the food and pharmaceutical industries.
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The objectives of the research are to extract chemicals from Algerian fir needles, to determine the total phenolics and flavonoids contents in ethylacetate fraction (EAF) and to ...assess the biological activities of the extract.
Several in-vitro biochemical assays were used to measure antioxidant and oxygen radical-scavenging abilities, and antibacterial activity was evaluated. Components of the extract were identified by mass spectrometry and comparison to reference compounds.
Results showed that EAF presented a powerful antioxidant activity in all assays and exhibited a potent inhibitory effect against all bacterial strains used. Fractionation and mass spectrometry identified a high amount of astragalin, hyperoside and quercitrin in the EAF from needles.
The best of our knowledge, we report here for the first time, the evaluation of biological activities and the determination of phytochemical profile of Algerian fir leaves extract underlying the importance of this species as a source of health-promoting.
Objective:To evaluate the essential oils(EO) composition,antimicrobial and antioxidant power of a local plant,Daucus gracilis(D.gracilis).Methods:The aerial parts of D.gracilis were subjected to ...hydro distillation by a Clevenger apparatus type to obtain the EO which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry,and screened for antimicrobial activity against five bacteria and three fungi by agar diffusion method.The mechanism of action of the EO was determined on the susceptible strains by both of time kill assay and lysis experience.The minimal inhibitory concentrations were determined by agar macrodilution and micro-dilution methods.Anti-oxidative properties of the EO were also studied by free diphenyl-2-picrylhydrazyl radical scavenging and reducing power techniques.Results:The EO yielded 0.68(v/w).The chemical analysis presented two dominant constituents which were the elemicin(35.3%) and the geranyl acetate(26.8%).D.gracilis EO inhibited the growth of Bacillus cereus and Proteus mirabilis significantly with minimal inhibitory concentrations of 17.15 μg/mL by the agar dilution method and57.05 μg/mL and 114.1 μg/mL,respectively by liquid micro-dilution.A remarkable decrease in a survival rate as well as in the absorbance in 260 nm was recorded,which suggested that the cytoplasm membrane was one of the targets of the EO.The EO showed,also,important anti-oxidative effects with an IC50 of 0.002 mg/mL and a dosedependent reducing power.Conclusions:D.gracilis EO showed potent antimicrobial and anti-oxidative activities and had acted on the cytoplasm membrane.These activities could be exploited in the food industry for food preservation.
Objective: To evaluate the chemical composition of the essential oil isolated from Elaeoselinum asclepium(L.) Bertol.(E. asclepium), and test the efficiency of the essential oil as an antimicrobial ...and antioxidant agent.Methods: Essential oil was obtained from the aerial parts of E. asclepium by hydro distillation and analyzed by gas chromatography and gas chromatography coupled with mass spectrometry. We study for the first time the chemical composition of the essential oil of E. asclepium, followed by the in vitro antimicrobial activities, which were evaluated by agar diffusion method against six Gram-positive bacteria, five Gram-negative bacteria, and two fungi. In addition, The antioxidant activities were also investigated using assays of 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and ferricreducing capacity.Results: The analyzed essential oil of the aerial parts of E. asclepium was rich in apinene(43.9%), other compounds detected in appreciable amounts were sabinene(27.9%) and b-pinene(16.0%). The essential oil yields 1.2%, the IC50 values of essential oil in 1,1-diphenyl-2-picrylhydrazyl assay in the reducing power assay were 48.26 mg/m L and at 1 mg/m L, respectively. The absorbance value of essential oil at 700 nm was0.956. The antimicrobial effect was higher on Candida albicans ATCC 1024 strain with the inhibition zone 14.5 mm than bacteria and molds.Conclusions: The essential oil of E. asclepium has antimicrobial and antioxidant activities. These species may be used as an important source of natural antimicrobial and antioxidant agents.
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