To evaluate the participation of the vessel wall in the pathogenesis of migraine attack, we measured the plasma levels of von Willebrand factor (vWF), a protein secreted from the endothelial cells.
...17 patients suffering from migraine without aura and 25 healthy volunteers were studied. von Willebrand factor and platelet aggregation tests were studied by conventional methods.
The levels of vWF:antigen increased from 72.4 +/- 29 U/dl in the intercrisis to 130.2 +/- 75 U/dl during the attack (p < 0.01). We did not detect difference in the platelet aggregability in both phases. Plasma vWF activity measured as ristocetin cofactor (vWF:RCo) was similar in intercrisis and crisis (100.6 +/- 31 U/dl vs 94.5 +/- 44 U/dl).
There is a plasma release of vWF molecules during the migraine crisis. This feature is not platelet dependent and is probably a consequence of endothelial stress.
Soluble class I antigens (sHLA) are secreted by lymphocytes upon activation in vitro. The intrathecal synthesis (ITS) of these molecules has been studied in patients with the varicella-zoster virus ...(VZV) meningitis. In this paper we describe a sHLA index IH = (CSF sHLA/serum sHLA): (CSF albumin/serum albumin) which is expected to increase only when sHLA is synthesised within the central nervous system (CNS). The IH is elevated in the first week of meningitis, when antibody synthesis is still low, and decreases thereafter. We think IH is an index of early lymphocyte activation within the CNS. The relation of these findings with previous in vitro studies is also discussed.
Botulism with sensory symptoms: a second case Martínez-Castrillo, J C; Del Real, M A; Hernandez Gonzalez, A ...
Journal of neurology, neurosurgery and psychiatry,
09/1991, Letnik:
54, Številka:
9
Journal Article
We have quantified the soluble class I antigen (sHLA) secretion by peripheral blood lymphocytes of 26 multiple sclerosis (MS) patients. Thirteen of them were in a stable phase of the disease, 6 on ...relapse and 7 suffered from a progressive MS. sHLA secretion was reduced in the presence or absence of phytohemagglutinin in patients with either active or stable MS, being normal after stimulation with a monoclonal antibody anti-CD3. In MS patients, lymphocyte proliferation and immunoglobulin secretion were found to be similar to those of 29 healthy blood donors who comprised the group of controls. These results reflect systemic anomalies in the cell activation process in MS, which seem to be independent of the disease activity. Whether these alterations are specific to MS or are common to other inflammatory CNS diseases remains to be elucidated.
sHLA are soluble class I antigens produced by lymphocytes on early activation. We have studied the sHLA index IH = (CSF sHLA/serum sHLA)/(CSF albumin/serum albumin), which reflects the intrathecal ...synthesis (ITS) of sHLA in 23 intravenous drug abusers with central nervous system (CNS) HIV infection. Their mean IH value was increased and directly correlated with ITS of IgG against HIV when the total group of patients was studied; however, 8 of them, who suffered from concomitant tuberculous meningitis, had a decreased IH. The relationship between this index, blood-brain barrier (BBB) function, and HIV and tuberculous infection was also studied. We consider IH an index of lymphocyte activation within the CNS. Its decrease in patients with CNS HIV infection may reflect the presence of a meningeal opportunistic infection due to Mycobacterium tuberculosis.
Two children with migraine showed low-density areas with an irregular enhancement pattern on CT scans of the head after contrast infusion. Angiography was normal in both patients. These abnormalities ...probably were due to transient blood-brain barrier damage. This knowledge is important when excluding other disorders like vascular malformations.
Soluble major histocompatibility complex class I molecules (sHLA) present in human serum can be resolved by gel filtration into two different peaks with an apparent molecular mass of about 200 kDa ...(30% of the total) and 50-60 kDa (60%-70%). The serological analysis of the peaks shows that A or B specificities can only be detected in the 200 kDa peak while both are recognized by the monomorphic W6/32 monoclonal antibody (mAb) and anti-beta 2-microglobulin mAb. Such sHLA (non HLA-A or -B) molecules are released from human spleen membranes upon incubation at 37 degrees C and have been purified by affinity chromatography with mAb W6/32 bound to Sepharose. The molecular mass analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the sHLA (non HLA-A or -B) and of the classical HLA-A or -B antigens still bound to the membranes and purified from the same membranes after detergent solubilization does not show a significant difference, indicating that sHLA do not represent proteolytic fragments of the classical HLA-A or -B antigens. The presence of sHLA (non HLA-A or -B) has also been detected in the supernatants of lymphocyte cultures and increases dramatically upon stimulation by mitogens. The effect of pokeweed mitogen, phytohemagglutinin, Staphylococcus aureus Cowan strain and phorbol 12-myristate 13-acetate on the secretion of sHLA has been studied. The molecular mass of the secreted sHLA (detected using 14Cleucine) is compared with the classical transmembrane proteins.
Recovery from rheumatoid cerebral vasculitis Gobernado, J M; Leiva, C; Rábano, J ...
Journal of neurology, neurosurgery and psychiatry,
04/1984, Letnik:
47, Številka:
4
Journal Article
Recenzirano
Odprti dostop
A 48-year-old woman with the classical features of longstanding rheumatoid arthritis acutely developed a diffuse cerebral disease. Angiograms showed extensive cerebral vasculitis, and CT scan ...demonstrated cerebral oedema and haemorrhage. Her clinical state, EEG and CT scan rapidly returned to normal following treatment with corticosteroids.
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