During their adult life stage most EPTs (Ephemeroptera, Plecoptera and Trichoptera) disperse by flying following the riparian corridor. Although it is likely that riparian forest fosters EPT ...dispersion, this has not been empirically tested in a larger dataset yet and several additional open questions remain. First, it is unclear if the effect of riparian vegetation on EPT community differs and depends on the spatial scale. Second, it is not assessed how the effect of riparian vegetation on EPTs is and how it changes depending on other environmental stressors. Third, the effect potentially depends on riparian vegetation characteristics such as trees species composition and cover.
We analysed 98 sites in lowland and lower mountain streams in Northrhine Westfalia, Germany, at two longitudinal and two lateral spatial scales. At each site we calculated the EPT community dispersal ability and quantified other environmental stressors as well as deciduous and coniferous woody cover in the riparian buffer. Generalised Linear Models were used to identify the conditions under which woody riparian vegetation has a significant effect on EPT community dispersal ability.
Our results confirmed that the share of weak dispersers increased with deciduous woody riparian cover in low mountain streams, indicating a potential positive effect of natural riparian forest on landscape connectivity. This relationship was only observed at the regional longitudinal scale irrespective of the lateral spatial scale. Tree species composition was relevant as coniferous forests did not contribute to this effect. Finally, there was some indication that the positive effect of deciduous riparian forest occurs at a moderate woody cover and levels off at higher values.
This highlights the role of riparian forests not only as habitat but also dispersal corridor in river management and the need to preserve and restore natural woody riparian vegetation to improve EPT communities and macroinvertebrates ecological status.
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•The effect of riparian forest on merolimnic stream insects dispersal was analysed.•Riparian forest drives the community at big spatial scales in mountain streams.•Deciduous forest correlates with weak dispersers, despite other stressors.•Coniferous forest correlates with strong fliers, possibly is a dispersal barrie.r•Deciduous forest increases weak dispersers until it reaches a certain cover.
Iron-fortified Cheddar cheese was manufactured with large microencapsulated ferrous sulfate (LMFS; 700–1,000 µm in diameter) or small microencapsulated ferrous sulfate (SMFS; 220–422 µm in diameter). ...Cheeses were aged 90 d. Compositional, chemical, and sensory characteristics were compared with control cheeses, which had no ferrous sulfate added. Compositional analysis included fat, protein, ash, moisture, as well as divalent cations iron, calcium, magnesium, and zinc. Thiobarbituric acid reactive species assay was conducted to determine lipid oxidation. A consumer panel consisting of 101 participants evaluated the cheeses for flavor, texture, appearance, and overall acceptability using a 9-point hedonic scale. Results showed 66.0% iron recovery for LMFS and 91.0% iron recovery for SMFS. Iron content was significantly increased from 0.030 mg of Fe/g in control cheeses to 0.134 mg of Fe/g of cheese for LMFS and 0.174 mg of Fe/g of cheese for SMFS. Fat, protein, ash, moisture, magnesium, zinc, and calcium contents were not significantly different when comparing iron-fortified cheeses with the control. Iron fortification did not increase lipid oxidation; however, iron fortification negatively affected Cheddar cheese sensory attributes, particularly the LMFS fortified cheese. Microencapsulation of ferrous sulfate failed to mask iron's distinct taste, color, and odor. Overall, SMFS showed better results compared with LMFS for iron retention and sensory evaluation in Cheddar cheese. Results of this study show that size of the microencapsulated particle is important in the retention of the iron in the cheese and its sensory attributes. This study provides new information on the importance of particle size with microencapsulated nutrients.
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•Lower freezing temperatures change the porus structure of CS-PVA hydrogels.•The CS-PVA hydrogels released diflunisal drug in controlled manner during 30 h.•A new mathematical model ...predicting physical and structural behavior is proposed.
The freezing-thawing is an advantageous method to produce hydrogels without crosslinking agents. In this study chitosan-poly(vinyl alcohol) (CS-PVA) hydrogels were prepared by varying the freezing conditions and composition, which affect the final characteristics of the products. The swelling degree, morphology, porosity, and diflunisal drug loading, as well as the drug release profiles were evaluated. The hydrogel swelling ratio was found to be mainly affected by the CS content, the number of freezing cycles and the temperature. SEM micrographs and porosity data confirm that pore size increases with the chitosan content. However, the use of either lower temperatures or longer freezing times, results in higher porosity and smaller pores. The drug release times of the CS-PVA hydrogels were as long as 30 h, and according to the mathematical fitting, a simple diffusion mechanism dominates the process. Moreover, a mathematical model predicting the hydrogels physical and structural behavior is proposed.
Large variations in wind energy production over a period of minutes to hours is a challenge for electricity balancing authorities. The use of reliable tools for the prediction of wind power and wind ...power ramp events is essential for the operator of the electrical system. The main objective of this work is to analyze the wind power and wind power ramp forecasting at Brazil and Uruguay. To achieve this goal the wavelet decomposition applying 48 different mother wavelet functions and deep learning techniques are used. The recurrent neural network was trained to perform the forecasting of 1 h ahead, and then, using it, the trained network was applied to recursively infer the forecasting for the next hours of the wind speed. After this computational procedure, the wind power and the wind power ramp were predicted. The results showed good accuracy and can be used as a tool to help national grid operators for the energy supply. The wavelet discrete Meyer family (dmey) demonstrates greater precision in the decomposition of the wind speed signals. Therefore, it is proven that the wavelet dmey is the most accurate in the decomposition of temporal wind signals, whether using signals from tropical or subtropical regions.
•Nowcasting wind prediction in tropical and subtropical sites using AI and Wavelet.•An ANN approach for the estimation of wind power ramp using deep learning.•Modeling of wind using atmospheric factors in tropical and subtropical sites.•Wind power and wind power ramp forecasting applying 48 mother Wavelet functions.
Specialized secretion systems are used by many bacteria to deliver effector proteins into host cells that can either mimic or disrupt the function of eukaryotic factors. We found that the ...intracellular pathogens Legionella pneumophila and Coxiella burnetii use a type IV secretion system to deliver into eukaryotic cells a large number of different bacterial proteins containing ankyrin repeat homology domains called Anks. The L. pneumophila AnkX protein prevented microtubule-dependent vesicular transport to interfere with fusion of the L. pneumophila-containing vacuole with late endosomes after infection of macrophages, which demonstrates that Ank proteins have effector functions important for bacterial infection of eukaryotic host cells.
Parents of many species care for their offspring by protecting them from a wide range of environmental hazards, including desiccation, food shortages, predators, competitors, and parasites and ...pathogens. Currently, little is known about the mechanisms and fitness consequences of parental defences against bacterial pathogens and competitors. Here, we combine approaches from microbiology and behavioural ecology to investigate the role and mechanistic basis of antibacterial secretions applied to carcasses by parents of the burying beetle Nicrophorus vespilloides. This species rears its larvae on vertebrate carcasses, where larvae suffer significant fitness costs due to competition with bacterial decomposers. We first confirm that anal secretions produced by parents are potently bactericidal and that their effects are specific to gram‐positive bacteria. Next, we identify the source of bacterial killing as a secreted lysozyme and show that its concentration changes throughout the breeding cycle. Finally, we show that secreted lysozyme is crucial for larval development, increasing survival by nearly two‐fold compared to offspring reared in its absence. These results demonstrate for the first time that anal secretions applied to carrion is a form of parental care and expand the mechanistic repertoire of defences used by parent insects to protect dependent offspring from microbial threats.
Since July 2009, there has been a community outbreak of leishmaniasis in the south-west area of the Madrid autonomous community, Spain, affecting residents from four towns that are geographically ...close together and share extensive park areas. As of December 2012, 446 cases were reported (6 in 2009, 97 in 2010, 196 in 2011 and 147 in 2012), a mean incidence rate of 22.2 per 100,000 inhabitants during July 2009 and December 2012. The mean age was 44 years (range: 2 months to 95 years); 61.0% were male. A total of 68 (15.2%) had immunosuppressive conditions; 160 (35.9%) had visceral leishmaniasis and 286 (64.1%) cutaneous. A total of 421 (94.4%) cases were confirmed. Leishmania infantum was identified as the agent. Monitoring revealed high densities of the vector Phlebotomus perniciosus. The surveillance system for canine leishmaniasis did not detect any increase in prevalence during the period. Environmental control measures have been taken, such as improvements in sanitation and disinsection in the risk areas and control of the overpopulation of Leporidae, as xenodiagnosis studies have shown that hares play a role as active reservoirs. This is the largest reported community outbreak of leishmaniasis in Europe. The discovery of the new reservoir stands out in the multifactorial aetiology of the outbreak. Epidemiological research and environmental intervention measures are continuing.
We present the synthesis and characterization of organic Salphen compounds containing bromine substituents at the para/ortho‐para positions, in their symmetric and non‐symmetric versions, and ...describe the X‐ray structure and full characterization for the new unsymmetrical varieties. We report for the first time antiproliferative activity in metal‐free brominated Salphen compounds, by evaluations in four human cancer cell lines, cervix (HeLa), prostate (PC‐3), lung (A549) and colon (LS 180) and one non‐cancerous counterpart (ARPE‐19). We assessed in vitro cell viability against controls using the MTT assay ((3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H‐tetrazolium bromide)) and determined the concentration required for 50 % growth inhibition (IC50), together with their selectivity vs. non‐cancerous cells. We found promising results against prostate (9.6 μM) and colon (13.5 μM) adenocarcinoma cells. We also found a tradeoff between selectivity (up to 3‐fold vs. ARPE‐19) and inhibition, depending upon the symmetry and bromine‐substitution of the molecules, showing up to 20‐fold higher selectivity vs. doxorubicin controls.
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•Yttria-stabilized zirconia custom photoresin containing zirconium and yttrium monomers has been developed.•The additive manufacturing of yttria-stabilized zirconia microarchitectures ...with sub-micrometer precision is presented.•Yttria stabilizes the cubic zirconium dioxide phase of the printed microarchitectures at low temperatures.•The mechanical properties help us understand the intrinsic mechanical strength of the yttria-stabilized zirconia microarchitectures.
The additive manufacturing (AM) of yttria-stabilized zirconia (YSZ) microarchitectures with sub-micrometer precision via two-photon lithography (TPL), utilizing custom photoresin containing zirconium and yttrium monomers is investigated. YSZ 3D microarchitectures can be formed at low temperatures (600 °C). The low-temperature phase stabilization of ZrO2 doped with Y2O3 demonstrates that doping ZrO2 with ≈ 10 mol% Y2O3 stabilizes the c-ZrO2 phase. The approach does not utilize YSZ particles as additives. Instead, the crystallization of the YSZ phase is initiated after printing, i.e., during thermal processing in the air at 600 °C – 1200 °C for one and two hours. The YSZ microarchitectures are characterized in detail. This includes understanding the role of defect chemistry, which has been overlooked in TPL-enabled micro-ceramics. Upon UV excitation, defect-related yellowish-green emission is observed from YSZ microarchitectures associated with intrinsic and extrinsic centers, correlated with the charge compensation due to Y3+ doping. The mechanical properties of the microarchitectures are assessed with manufactured micropillars. Micropillar compression yields the intrinsic mechanical strength of YSZ. The highest strength is observed for micropillars annealed at 600 °C, and this characteristic decreased with an increase in the annealing temperature. The deformation behavior gradually changes from ductile to brittle-like, correlating with the Hall–Petch strengthening mechanism.
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