Fifty-three patients with standard risk leukemia who underwent allogeneic peripheral blood stem cell transplantation (alloPBSCT) from their HLA-identical siblings were analyzed for engraftment, ...incidence and severity of GVHD, and relapse rate. Standard risk leukemia was defined as AML in first complete remission or CML in first chronic phase within the first year after diagnosis. The median age was 34.5 years (range 13-47). Stem cells were mobilized by using 10 microg/kg G-CSF subcutaneously for 5 days. A median of 5. 7 (2.1-21.4) x 106/kg CD34+ cells was collected over a median of 2 (range 1-5) apheresis procedures. Cyclosporin A (CsA) plus short-course MTX were used for GVHD prophylaxis. Recovery to granulocytes >0.5 x 109/l and platelets >20 x 109/l occurred at a median of day +13 (range 8-32) and +13 (range 8-51), respectively. Day +100 transplant-related mortality was 13.2% (7/53). Acute GVHD occurred in 20 of 49 (41%) evaluable patients and only six (12.3%) of them had severe disease (grade III-IV). Chronic GVHD occurred in 30 of 42 (71.4%) evaluable patients. Relapse rate at 2 years was 7. 5%. The median overall and leukemia-free survivals were 22 (4-44) and 20 (3-44) months, respectively. Estimated 4 year leukemia-free and overall survival rates were 60% and 62%, respectively. In conclusion, alloPBSCT in standard risk leukemia seems to be associated with a low relapse rate and no increased risk of acute GVHD, but there is a trend for higher incidence of cGVHD. Bone Marrow Transplantation (2000) 25, 1229-1232.
Objective: In this study, we investigated the relationship between chronic low back pain and bone mineral density in a young and middle-aged male population. Materials and Methods: A total of 104 ...male patients with chronic low back pain were enrolled in the study. All subjects completed the Oswestry Disability Index (ODI). Additionally, the intervertebral disc degeneration (DD) was graded according to the Thompson's classification. After bone mineral density was measured the patients were divided into two groups: normal (n=50) and low (n=54) bone density groups. In addition, for comparing the data of patient sub-groups, bone mineral density was measured in 30 healthy subjects. Results: T and Z-scores of the L2 -L4 and the femoral neck were found to be significantly lower in the patient group compared to the control group. In the patient group, there was a positive correlation between the ODI score and the DD score. There were negative correlations between both the ODI score and the DD score and L2-L4 and femoral neck T scores and Z-score. The DD and ODI scores were found to be significantly higher in a patient with low bone density compared to a patient with normal bone density. Conclusion: We think that the low bone mineral density in young and middle-aged male patients with chronic lower back pain is related to disability and physical inactivity. Key Words: Chronic low back pain; lumbar disc degeneration; bone mineral density; physical inactivity; disability; male Amac: Bu calismada genc ve orta yas erkek populasyonunda kronik bel agrisi ve kemik mineral yogunlugu arasindaki iliski incelenmistir. Gerec ve Yontem: Calismaya alinan kronik bel agrili toplam 104 erkek hasta Oswestry Disability Index (ODI) sorgulama formunu doldurdu. Ayrica, intervertebral disk dejenerasyonu Thompson's klasifikasyonuna gore derecelendirildi. Hastalar kemik mineral yogunlugu (KMY) olculdukten sonra normal kemik dansiteli (n=50) ve dusuk kemik dansiteli (n=54) olmak uzere iki gruba ayrildi. Ek olarak hasta alt gruplarinin verileri ile karsilastirilmak uzere 30 sagliklida da KMY olcumu yapildi. Bulgular: L2-L4 ve femur boynu T ve Z skorlari, hasta grubunda kontrol grubuna kiyasla anlamli derecede daha dusuk olarak bulundu. Hasta grubunda, ODI skoru ve DD skoru arasinda pozitif korelasyon vardi. Hem ODI skoru hem de DD skoru ile L2-L4 ve femur boynu T ve Z skorlari arasinda negatif korelasyonlar vardi. DD ve ODI skorlari normal kemik dansiteli hasta ile kiyaslandiginda, dusuk KMY'li hastada anlamli derecede daha yuksek olarak bulundu. Sonuc: Biz, kronik bel agrili genc ve orta yas erkek hastalardaki dusuk KMY'nin, fiziksel aktivite azligi ve yetersizlige bagli oldugunu dusunuyoruz. Anahtar Kelimeler: Kronik bel agrisi; lomber disk dejenerasyonu; kemik mineral yogunlugu; fiziksel inaktivite; ozurluluk; erkek
In the present study, subclinical lesion involvement was investigated using the P50 component in Behcet`s patients without neurological manifestation.
We performed this clinical research in Erciyes ...University, Faculty of Medicine between December 2000 and November 2001. The studies were carried out on 18 Behcet`s patients without neurologic findings and 18 volunteers for control. Standard Ag/AgCl electrodes in plastic cups were used for monopolar EEG derivations. They were attached with electrode paste and tape at the Cz (vertex) according to the 10-20 system. The auditory stimuli were delivered in pairs. The P50 waves, which may be taken from approximately 50 msec from the stimuli, were collected by computer system. Amplitudes and latencies of the P50 components were measured in the same system.
This study showed that the suppression of P50 responses performed by the test stimuli, was significantly more decreased in Behcet`s patients than the control subjects.
The decrease of the suppression of the auditory P50 response to repeated stimuli reflects a deficit in the central nervous system`s ability, such as attention, cognition, and sensory input in Behcet`s patients and can be used as a neurophysiological marker in subclinical lesions in these patients.
The effect of granulocyte colony-stimulating factor (G-CSF) on peripheral blood lymphocytes (PBL) and CD34+ cell frequency in the apheresis product has been determined in 25 healthy stem cell donors. ...Peripheral blood mononuclear cells (PBMNC) were collected after five days of G-CSF 10 μg/kg/day s.c., which was well tolerated. The median number of leukocytes increased eight-fold over that of pretreatment levels. Collection of PBMNC lasted a median of two (range, 1–3) days. The mean mononuclear cell (MNC) count and total lymphocyte percentage were 6.69×10
8/kg and 59.08%, respectively, and the frequency of CD34+ cell expression was 2.1% in the apheresis product. The frequency of CD3+, CD4+, CD25+, NK and CD122+ cell expressions in mobilized PBMNC and PBL showed no significant difference. However, the frequency of CD8+, CD8+28+, CD3+DR+, CD19+, CD20+ and CD22+ B cells expression in the apheresis product increased significantly compared to steady-state PBL. In contrast, the frequency of the CD11a+ and CD8+38+ cell expressions in the apheresis product was decreased compared to the steady-state PBL. The mean yield of CD34+ and CD3+ cells were 13.6×10
6 and 2.69×10
8/kg of recipient body weight (RBW), respectively. Following allograft all patients engrafted with >0.5×10
9/l neutrophil and ⩾20×10
9/l platelets on a median of day 13 and 12, respectively. Nine patients had grade II–IV acute GVHD and chronic GVHD occurred in eight patients. Four patients died due to transplant-related complications. There was one late engraftment failure which occurred on the fifth month. Thirteen patients are still alive. In conclusion, these results indicate that administration of G-CSF at 10 μg/kg/day in normal donors alters the lymphocyte subsets and there are significant differences in the lymphocyte contents of the recipients before apheresis and in apheresis product.
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