While the international nEDM collaboration at the Paul Scherrer Institut (PSI) took data in 2017 that covered a considerable fraction of the parameter space of claimed potential signals of ...hypothetical neutron (n) to mirror-neutron (n′) transitions, it could not test all claimed signal regions at various mirror magnetic fields. Therefore, a new study of n−n′ oscillations using stored ultracold neutrons (UCNs) is underway at PSI, considerably expanding the reach in parameter space of mirror magnetic fields (B′) and oscillation time constants (τnn′). The new apparatus is designed to test for the anomalous loss of stored ultracold neutrons as a function of an applied magnetic field. The experiment is distinguished from its predecessors by its very large storage vessel (1.47 m3), enhancing its statistical sensitivity. In a test experiment in 2020 we have demonstrated the capabilities of our apparatus. However, the full analysis of our recent data is still pending. Based on already demonstrated performance, we will reach sensitivity to oscillation times τnn′/cos(β) well above a hundred seconds, with β being the angle between B′ and the applied magnetic field B. The scan of B will allow the finding or the comprehensive exclusion of potential signals reported in the analysis of previous experiments and suggested to be consistent with neutron to mirror-neutron oscillations.
Capsular polysaccharides (CPSs) are protective structures on the surfaces of many Gram-negative bacteria. The principal CPS of the human pathogen and Tier 1 select agent Burkholderia pseudomallei ...consists of a linear repeat of --3)--2-O-acetyl-6-deoxy-β-d-manno-heptopyranose-(1-. This CPS is critical to the virulence of this emerging pathogen and represents a key target for the development of novel therapeutics. wcbI is one of several genes in the CPS biosynthetic cluster whose deletion leads to significant attenuation of the pathogen; unlike most others, it has no homologues of known function and no detectable sequence similarity to any protein with an extant structure. Here, the crystal structure of WcbI bound to its proposed product, coenzyme A, is reported at 1.38 Å resolution, solved using the halide-soak method with multiple anomalous dispersion. This structure reveals that WcbI incorporates a previously described 100-amino-acid subdomain into a novel, principally helical fold (310 amino acids). This fold adopts a cradle-like structure, with a deep binding pocket for CoA in the loop-rich cradle. Structural analysis and biophysical assays suggest that WcbI functions as an acetyltransferase enzyme, whilst biochemical tests suggest that another functional module might be required to assist its activity in forming the mature B. pseudomallei capsule.
Developments of seamless mobile services are faced with two broad challenges, systems security and user privacy - access to wireless systems is highly insecure due to the lack of physical boundaries ...and, secondly, location based services (LBS) could be used to extract highly sensitive user information. In this paper, we describe our work on developing systems which exploit location information to enhance security and services under privacy constraints. We describe two complimentary methods which we have developed to track node location information within production University Campus Networks comprising of large numbers of users. The location data is used to enhance security and services. Specifically, we describe a method for creating geographic firewalls which allows us to restrict and enhance services to individual users within a specific containment area regardless of physical association. We also report our work on LBS development to provide visualization of spatio-temporal node distribution under privacy considerations.
Retrospective review from a single institution.
To investigate the effect of hip osteoarthritis (OA) on spinopelvic compensatory mechanisms as a result of reduced hip range of motion (ROM) between ...sitting and standing.
Hip OA results in reduced hip ROM and contracture, causing pain during postural changes. Hip flexion contracture is known to reduce the ability to compensate for spinal deformity while standing; however, the effects of postural spinal alignment change between sitting and standing is not well understood.
Sit-stand radiographs of patients without prior spinal fusion or hip prosthesis were evaluated. Hip OA was graded by Kellgren-Lawrence grades and divided into low-grade (LOA; grade 0-2) and severe (SOA; grade 3 or 4) groups. Radiographic parameters evaluated were pelvic incidence (PI), pelvic tilt (PT), lumbar lordosis (LL), PI-LL, thoracic kyphosis (TK), SVA, T1-pelvic angle (TPA), T10-L2, proximal femoral shaft angle (PFSA), and hip flexion (PT change-PFSA change). Changes in sit-stand parameters were compared between LOA and SOA groups.
548 patients were included (LOA = 311; SOA = 237). After propensity score matching for age, body mass index, and PI, 183 LOA and 183 SOA patients were analyzed. Standing analysis demonstrated that SOA had higher SVA (31.1 vs. 21.7), lower TK (-36.2 vs. -41.1), and larger PFSA (9.1 vs. 7.4) (all p < .05). Sitting analysis demonstrated that SOA had higher PT (29.7 vs. 23.3), higher PI-LL (21.6 vs. 12.4), less LL (31.7 vs. 41.6), less TK (-33.2 vs. -38.6), and greater TPA (27.9 vs. 22.5) (all p < .05). SOA had less hip ROM from standing to sitting versus LOA (71.5 vs. 81.6) (p < .05). Therefore, SOA had more change in PT (15.2 vs. 7.3), PI-LL (20.6 vs. 13.7), LL (-21.4 vs. -13.1), and T10-L2 (-4.9 vs. -1.1) (all p < .001), allowing the femurs to change position despite reduced hip ROM. SOA had greater TPA reduction (15.1 vs. 9.6) and less PFSA change (86.7 vs. 88.8) compared with LOA (both p < .001).
Spinopelvic compensatory mechanisms are adapted for reduced hip joint motion associated with hip OA in standing and sitting.
Level III.
Diversity and distribution of Victoria Land biota Adams, Byron J.; Bardgett, Richard D.; Ayres, Edward ...
Soil biology & biochemistry,
10/2006, Letnik:
38, Številka:
10
Journal Article, Conference Proceeding
Recenzirano
Understanding the relationship between soil biodiversity and ecosystem functioning is critical to predicting and monitoring the effects of ecosystem changes on important soil processes. However, most ...of Earth's soils are too biologically diverse to identify each species present and determine their functional role in food webs. The soil ecosystems of Victoria Land (VL) Antarctica are functionally and biotically simple, and serve as in situ models for determining the relationship between biodiversity and ecosystem processes. For a few VL taxa (microarthropods, nematodes, algae, mosses and lichens), species diversity has been intensively assessed in highly localized habitats, but little is known of how community assemblages vary across broader spatial scales, or across latitudinal and environmental gradients. The composition of tardigrade, rotifer, protist, fungal and prokaryote communities is emerging. The latter groups are the least studied, but potentially the most diverse. Endemism is highest for microarthropods and nematodes, less so for tardigrades and rotifers, and apparently low for mosses, lichens, protists, fungi and prokaryotes. Much of what is known about VL diversity and distribution occurs in an evolutionary and ecological vacuum; links between taxa and functional role in ecosystems are poorly known and future studies must utilize phylogenetic information to infer patterns of community assembly, speciation, extinction, population processes and biogeography. However, a comprehensive compilation of all the species that participate in soil ecosystem processes, and their distribution across regional and landscape scales is immediately achievable in VL with the resources, tools, and expertise currently available. We suggest that the soil ecosystems of VL should play a major role in exploring the relationship between biodiversity and ecosystem functioning, and in monitoring the effects of environmental change on soil processes in real time and space.
Abstract
The commercial Dungeness crab (Metacarcinus magister) fishery in Oregon and Washington (USA) is one of the most valuable fisheries in the region, but it experiences high interannual ...variability. These fluctuations have been attributed to environmental drivers on seasonal and annual timescales. In this study, researchers and state and tribal fisheries managers develop a statistical model for Dungeness crab catch per unit effort (CPUE) to help inform dynamic management decisions in Oregon and Washington. Fishing observations were matched to seasonally forecast and lagged ocean conditions from J-SCOPE, a regional forecast system (http://www.nanoos.org/products/j-scope/). Inclusion of dynamic and lagged ocean conditions improved model skill compared to simpler models, and the best model captured intraseasonal trends and interannual variability in catch rates, and spatial catch patterns. We also found that model skill relied on fishing behaviour, which varies interannually, highlighting the need for advanced fishing behaviour modelling to reduce uncertainty. The relationships between catch rates and ocean conditions may help elucidate environmental influences of catch variability. Forecast products were co-designed with managers to meet their needs for key decision points. Our results illustrate a seasonal forecasting approach for management of other highly productive, but also dynamic, invertebrates that increasingly contribute to global fisheries yield.
The upcoming Facility for Rare Isotope Beams (FRIB) at Michigan State University provides a new opportunity to access some of the world's most specialized scientific resources: radioisotopes. An ...excess of useful radioisotopes will be formed as FRIB fulfills its basic science mission of providing rare isotope beams. In order for the FRIB beams to reach high-purity, many of the isotopes are discarded and go unused. If harvested, the unused isotopes could enable new research for diverse applications ranging from medical therapy and diagnosis to nuclear security. Given that FRIB will have the capability to create about 80% of all possible atomic nuclei, harvesting at FRIB will provide a fast path for access to a vast array of isotopes of interest in basic and applied science investigations. To fully realize this opportunity, infrastructure investment is required to enable harvesting and purification of otherwise unused isotopes. An investment in isotope harvesting at FRIB will provide a powerful resource for development of crucial isotope applications. In 2010, the United States Department of Energy Office of Science, Nuclear Physics, sponsored the first 'Workshop on Isotope Harvesting at FRIB', convening researchers from diverse fields to discuss the scientific impact and technical feasibility of isotope harvesting. Following the initial meeting, a series of biennial workshops was organized. At the fourth workshop, at Michigan State University in 2016, the community elected to prepare a formal document to present their findings. This report is the output of the working group, drawing on contributions and discussions with a broad range of scientific experts.
Study Design:
This was a single-center retrospective review.
Objectives:
To explore how age and gender affect PROMIS scores compared with traditional health-related quality of life (HRQL) in spine ...patients.
Methods:
Patients presenting with a primary complaint of back pain (BP) or neck pain (NP) were included. Legacy HRQLs were Oswestry Disability Index (ODI), Neck Disability Index (NDI), and Visual Analogue Scale (VAS). PROMIS Physical Function (PF), Pain Intensity (Int), and Pain Interference (Inf) were also administered to patients in a clinical setting. Patients were grouped by chief complaint, age (18-44, 45-64, 65+ years) and gender. Two parallel analyses were conducted to identify the effects of age and gender on patient-reported outcomes. Age groups were compared after propensity-score matching by VAS-pain and gender. Separately, genders were compared after propensity-score matching by age and VAS-pain.
Results:
A total of 484 BP and 128 NP patients were matched into gender cohorts (n = 201 in each BP group, 46 in each NP group). Among BP patients, female patients demonstrated worse disability by ODI (44.15 vs 38.45, P = .005); PROMIS-PF did not differ by gender. Among NP patients, neither legacy HRQLs nor PROMIS differed by gender when controlling for NP and age. BP and NP patients were matched into age cohorts (n = 135 in each BP group and n = 14 in each BP group). Among BP patients, ANOVA revealed differences between groups when controlling for BP and gender: ODI (P < .001), PROMIS-PF (P = .018), PROMIS-Int (P < .001) PROMIS-Inf (P < .001). Among NP patients, matched age groups differed significantly in terms of NDI (P = .032) and PROMIS-PF (P = .022) but not PROMIS-Int or PROMIS-Inf.
Conclusions:
Age and gender confound traditional HRQLs as well as PROMIS domains. However, PROMIS offers age and gender-specific scores, which traditional HRQLs lack.
C5 palsy stands as a known but poorly understood complication of cervical spine surgery with rates reported as high as 30%. Although there are multiple studies presenting different risk factors, ...variability in findings have led to controversy in the etiology of postoperative C5 palsy.
To evaluate factors that predispose a patient to developing C5 palsy following cervical spine surgery.
Retrospective review of a large single center academic institution.
This study included 239 cervical spinal procedures from 2013 to 2018.
Patient demographics, surgical procedure and approach, perioperative clinical characteristics, postoperative rate of C5 palsy, C5 palsy resolution, and radiographic outcomes.
Patients ≥18 years of age who underwent cervical spinal surgery including the C4/C5 level, with minimum 1-year follow-up were included. C5 palsy was defined as deltoid +/- bicep weakness with Modified Rankin Scale grading at least 1 point below baseline (BL). Characteristics studied include: demographics, surgical procedure and approach (decompression and/or fusion). Radiographic analyses of MRI and CT measurements of foramen dimensions and spinal cord drift at BL and follow-up were performed. Statistical analyses included t-tests, chi-square analysis, and multivariate logistic regression to determine independent predictive factors. A receiver operating characteristic curve was run to determine the cutoff levels for predictive factors. Significance set at p<0.05.
A total of 239 patients were included, with a C5 palsy rate of 6.3% for all cases. Subjects that developed C5 palsy were older (64.67±8.61 vs 57.56±11.61, p=0.021), had more levels decompressed posteriorly (3.20±1.82 vs 1.12±1.74, p<0.001), a higher rate of preoperative myelomalacia (60% vs 19.6%, p=0.008), and higher CCI (3.80±1.97 vs 2.35±1.95, p=0.005). Anterior cervical discectomy and fusion (ACDF) surgeries had a 2.4% rate of C5 palsy while the posterior approach rate was 11.9% (p<0.007). The highest rate was in laminectomy and posterior fusion at 15.2% (p<0.001). There were no significant differences in palsy resolution between those treated surgically compared to those not treated. Radiographically, C5 palsy patients had smaller postoperative foramen width measurements than those who did not develop C5 palsy (2.16±1.04 vs 3.31±1.25, p=0.023 on the right; 1.84±0.80 vs 3.02±1.25, p=0.014 on the left). When controlling for technique, CCI, age, BMI, and myelomalacia, multivariate regression analysis revealed increasing levels of laminectomy to be a significant independent predictor of C5 palsy (1.602 (2.258-1.136) for each additional level decompressed, p=0.007). The cut-off value for levels decompressed via laminectomy was determined to be 3.50 levels (≥0.7 AUC ≤0.8). Postoperative foramen width was also an independent risk factor for C5 palsy (0.009 (0.000-0.726), p=0.035), it was determined that risk of C5 palsy increases at <2.625mm foramen width (≥0.8 AUC ≤0.9).
C5 palsy following cervical spine surgery is correlated with residual postoperative foraminal stenosis and posterior surgery with increasing number levels decompressed. Patients undergoing more than 3.5 level laminectomy with less than 2.625mm foramen width postoperatively are at highest risk of developing C5 palsy.
This abstract does not discuss or include any applicable devices or drugs.
The thoracolumbar junction poses increased risk for high stress on upper lumbar discs when not adequately fused. However, controversy persists. This study evaluates outcomes of upper instrumented ...vertebrae (UIV) selection in the thoracic versus the upper lumbar spine when fusing to the sacrum.
To compare revision rates for thoracolumbar fusions to the sacrum and pelvis at 1- and 2-year follow-up, stratified by UIV selection.
Retrospective cohort study at a single institution.
A total of 968 patients who had thoracolumbar fusion surgery between 2012-2018 with at least one year of postoperative follow-up.
Reoperation rates from 1 and 2 years after revision surgery, reoperation diagnoses, body mass index (BMI), gender, Charleston Comorbidity Index (CCI), perioperative complications, ASA grade (ASA), operative time (OT), and blood loss (EBL).
A retrospective review of patients undergoing revision spinal fusion with lower instrumented vertebra of S1 or pelvis between 2012-2018 at a single institution was performed. Patients with less than 1 year of follow-up after their index procedure were excluded from the analysis. Patients were categorized based on UIV into 6 groups: T9-T11, L1, L2, L3, L4, and L5. Demographic and operative data were collected and compared between the different UIV groups in each cohort using chi-squared and ANOVA tests. Revision rates at 1- and 2-year follow-up and the reasons for revision were compared between groups.
There were 168 revision spinal fusions that reached 1-year follow-up, with 54 having UIV at T9-T11, 2 fusions L1 to sacrum, 26 fusions L2 to sacrum, 25 fusions L3 to sacrum, 36 fusions L4 to sacrum, 23 fusions L5 to sacrum. There was significant difference in patient age, with oldest in the L1 group (65.5±3.5) and youngest in the L5 group (51±15.4 p<0.001). There was significant difference in gender as both cases in L2 was female and L5 fusions only had 39.1% females (p = 0.006). There were no differences in BMI, CCI, and ASA. EBL (1891.6mL±1226.9mL p <0.001) and length of stay (7.7±3.0 p <0.001) was highest in the T9-11 UIV group. Operative time was highest in the L1 (464.5±174.7 p<0.001) UIV group. Levels added on top of prior fusion were calculated by subtracting UIV of index fusion to past UIV. Adding on 2 levels had a 1-year revision rate of 14.3% (p = 0.032), while adding on 1 level had a 0% 1-year revision rate. Adding on 4 levels had a revision rate of 12.5%, and adding on 7 levels had a 50% 1-year revision rate. At 2 years, adding on 1 level had revision rate of 25% and adding on 2 levels had a revision rate of 20% (p = 0.769). There is no statistically significant difference in perioperative complication rates between UIV groups (p = 0.114). The reoperation rate at 1 year for all levels was 9% and highest in UIV at L1 (50%, p=0.06). At 2-year follow-up, the reoperation rate for the total cohort was 32.34% with the highest revision rate for fusion from L2 to sacrum (53.85%) followed by (T9-T11 sacrum fusion 42.31%, L4 to sacrum31.71%, L3 to sacrum 30%, L5 to sacrum 25.04%, L1 to sacrum 25%, p=0.195). Grouping the UIV into lower thoracic (T9-T11), upper lumbar (L2-L3), and lower lumbar (L4-L5) showed similar revision rates at one year (p=0.697). At two years, lower thoracic group had a revision rate of 40% versus, 23.3% in the upper lumbar group (p =0.399).
There is no statistically significant difference in reoperation rates for revision thoracolumbar fusions to the sacrum/pelvis associated with different UIV selection.
This abstract does not discuss or include any applicable devices or drugs.
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