Introduction
:
Streptococcus mutans
est une bactérie principalement responsable de la maladie carieuse. Sur le plan socioéconomique trouver un moyen simple et peu coûteux s’avère nécessaire. Cette ...étude vise à évaluer l’effet antibactérien et la détermination de la concentration minimale inhibitrice de la curcumine synthétique et naturelle sur des souches de
Streptococcus mutans
en mode biofilm.
Matériels et méthodes
: La souche de
Streptococcus mutans
a été isolée à partir de prélèvements salivaires à l’aide d’un kit spécial (CRT Bacteria® d’Ivoclar) au service d’odontologie conservatrice endodontie du CHU de Tlemcen. La détermination de la concentration minimale inhibitrice a été faite par la technique de dilution en microplaques de 96 puits, avec des dilutions en série double de curcumine synthétique (Sigma- Aldrich, Saint-Quentin-Fallavier, France) et de curcumine naturelle (curcumine pure à 99 %, origine Inde), préparées dans le Bouillon Cœur-Cervelle® (BHIB) (Conda Pronadisa, Madrid, Spain) jusqu’à un volume final de 100 μl par puits. Puis une observation par un microscope électronique à balayage environnementale a été faite sur des lames de verre immergées dans une suspension de
Streptococcus mutans
incubées 48 heures à 37 °C et plongées pendant 30 minutes dans une solution de curcumine à sa concentration minimale inhibitrice.
Résultats
: La concentration minimale inhibitrice de la curcumine est de 64 μg/ml, et la microscopie électronique à balayage a montré une réduction significative du nombre des souches
Streptococcus mutans
adhérées.
Conclusion
: La curcumine est un agent antibactérien prometteur pour la prévention de la maladie carieuse.
Introduction
:
Streptococcus mutans
is a bacterium mainly responsible for carious disease. From a socioeconomic point of view, a simple and inexpensive solution is necessary. This study aims to evaluate the antibacterial effect and determine the determination of the minimum inhibitory concentration of synthetic and natural curcumin on strains of
Streptococcus mutans
in biofilm mode.
Materials and methods
: The
Streptococcus mutans
strain was isolated from saliva samples using a special kit (CRT Bacteria® Ivoclar) at the department of conservative endodontics of CHU Tlemcen. The determination of the minimum inhibitory concentration was made by the dilution technique in 96-well microplates with double serial dilutions of synthetic curcumin (Sigma-Aldrich, Saint-Quentin- Fallavier, France) and natural curcumin (99% pure curcumin from India), prepared in Brain Heart Broth (BHIB) (Conda Pronadisa, Madrid, Spain) up to a final volume of 100 μl per well. Then an observation by an environmental scanning electron microscope was made on glass slides immersed in a suspension of
Streptococcus mutans
incubated for 48 hours at 37 °C and immersed for 30 minutes in a curcumin solution at its minimum inhibitory concentration.
Results
: The minimum inhibitory concentration of curcumin is 64 μg/ml and scanning electron microscopy has shown a significant reduction in the number of
Streptococcus mutans
strains adhered.
Conclusion
: Curcumin is a promising antibacterial agent for the prevention of carious disease.
Many moderate to large historical and instrumental events have been documented in the Mitidja basin, affecting in particular the southern edge of the active Quaternary Mitidja basin, which is ...composed of nearly NE–SW-trending fault system. The earthquake catalogue reports that the Hammam Melouane region has experienced several moderate seismic events such as those of 8 February 1937 (
I
0
= V), 20 July 1975 (
I
0
= V–VI), 29 September 1981 (
I
0
= V) and 17 December 1986 (
I
0
= V). Recently, between 2013 and 2016, this area experienced three moderate earthquakes with a series of aftershocks. In this paper, we present an analysis of the seismic sequences that occurred in the Hammam Melouane Geothermal Spring area on 17 July 2013 (
M
L
4.9), 23 December 2014 (
M
L
5.3) and 10 February 2016 (
M
w
4.8), about 3–7 km apart, at hypocentral depths of 11.5 km, 19.0 km and 18.0 km, respectively, with centroid depth of 5 km for the first main shock. Ninety-seven events with local magnitude ranging between
M
L
0.9 and
M
L
5.3 were recorded and analyzed. The series of aftershocks display two clusters, trending N–S for the 2013 event and NE–SW for the 2014 event, located at shallow depths of 1.5–14 km and 18–28 km, respectively. The event distribution shows variability in faulting, combining strike-slip and thrust focal mechanisms of the main events, leading us to hypothesize a simultaneous interaction between two geological active structures represented by the ~ N–S- to NE–SW-trending faults belonging to the southern Mitidja fault system. On one hand, the computed ΔCFF indeed supports and strengthens the fault interaction model between the three events. On the other hand, the analysis of the post-seismic stress distribution caused by fluid circulation reveals that the 2013 and 2014 events seem to have caused a poroelastic stress relaxation and thus influenced the occurrence of the 2016 main shock.
On August 1st, 2014, a moderate-sized earthquake struck the capital city of Algiers at 05:11:17.6 (GMT+1). The earthquake caused the death of six peoples and injured 420, mainly following a panic ...movement among the population. Following the main shock, we surveyed the aftershock activity using a portable seismological network (short period), installed from August 2nd, 2014 to August 21st, 2015. In this work, first, we determined the main shock epicenter using the accelerograms recorded by the Algerian accelerograph network (under the coordination of the National Center of Applied Research in Earthquake Engineering–CGS). We calculated the focal mechanism of the main shock, using the inversion of the accelerograph waveforms in displacement that provides a reverse fault with a slight right-lateral component of slip and a compression axis striking NNW–SSE. The obtained scalar seismic moment (
M
o
= 1.25 × 10
17
Nm) corresponds to a moment magnitude of
M
w
= 5.3. Second, the analysis of the obtained aftershock swarm, of the survey, suggests an offshore ENE–WSW, trending and NNW dipping, causative active fault in the bay of Algiers, which may likely correspond to an offshore unknown segment of the Sahel active fault.
The zinc transporter 8 (ZnT8) is an islet β-cell secretory granule membrane protein coded by the SLC30A8 gene, identified as a novel autoantigen in human type 1 diabetes (T1D). As no data of ZnT8ab ...in Algerian patients have been reported, we aim to evaluate the prevalence of ZnT8ab in young Algerians with T1D and determine whether ZnT8ab could be a better diagnostic tool to replace the other conventional autoantibodies detected in patients with type 1 diabetes. For this purpose, we evaluated the prevalence of islets cells antibodies (ICA), glutamic acid decarboxylase (GAD), islet antigen type 2 (IA2), insulin (IA) autoantibodies (ab) and for the first time in Algeria, the zinc transporter 8 (ZnT8) in young Algerian patients with type 1 diabetes.
In our cross-sectional study, 160 patients between 1 and 35 years old, diagnosed with type 1 diabetes were enrolled. ICAab was analyzed by indirect immunofluorescence (IIF), GADab, IA2ab, IAab and ZnT8ab were analyzed by ELISA, fasting blood glucose was performed by enzymatic method (glucose-oxidase) and HbA1c by turbid metric method.
Our cohort was composed with 74 males and 86 females (OR=1.16); the mean of age was 14.09 1–35 years old and the median diabetes duration was 4.10 1–18 years. Our cohort had a mean of HbA1c of 9.22 5.40–15%, the mean of birth weight was 3360.52 2200–4800g; the mean of BMI was 19.30 16.04–22.46kg/m2. Out of 160 patients, 44 (27.5%) were under mother breastfeeding and 116/160 (72.5%) were under artificial feeding. One antibody, at least, was found in 94.38% and the ZnT8ab was significantly more positive in females (70.3%) than in males (10.7%) (***P=8.033×10−15). The concentration of ZnT8ab was higher in females than in males (females=122.25UI/mL versus males=51.38UI/mL; *P=0.03); ICAab, GADab and ZnT8ab were more present in patients with consanguineous parents (***P=0.0002, *P=0.019 and *P=0.03; respectively)
Our study on ZnT8ab in T1D is the first in the Maghreb region and we observed a prevalence of 46.25%. The positivity of ZnT8ab enabled us to classify in T1DA 50% of diabetics with obvious T1D phenotype and negative routine autoantibodies, thus ZnT8ab is a good tool for differential diagnosis of type 1 diabetes. According to our results, a simultaneous analysis for ZnT8 and IA2 autoantibodies can be a better and efficient diagnosis of type 1A diabetes from the beginning of the disease.
The aims of this study were first to clearly define two different entities: locoregional recurrences and limited metastatic disease and secondly to evaluate the place of extensive surgery in these ...two types of recurrence. Material and Methods. Twenty-four patients were followed from June 2004 until May 2014. All patients underwent surgery but for 1 patient this surgery was stopped because the tumour was unresectable. Results. The median interval between surgery for the primary tumour and the locoregional recurrence or metastatic evolution was 129 months. Eight patients had pure nodal recurrences, 4 had nodal and muscular recurrences, 5 had muscular + skin recurrences, and 8 had metastatic evolution. Currently, all patients are still alive but 2 have liver metastases. Disease free survival was measured at 2 years and extrapolated at 5 years and was 92% at these two time points. No difference was observed for young or older women; limited metastatic evolution and locoregional recurrence exhibited the same disease free survival. Conclusion. Extensive surgery has a place in locoregional and limited metastatic breast cancer recurrences but this option must absolutely be integrated in the multidisciplinary strategy of therapeutic options and needs to be planned with a curative intent.
On August 1st, 2014, a moderate-sized earthquake struck the capital city of Algiers at 05:11:17.6 (GMT+1). The earthquake caused the death of six peoples and injured 420, mainly following a panic ...movement among the population. Following the main shock, we surveyed the aftershock activity using a portable seismological network (short period), installed from August 2nd, 2014 to August 21st, 2015. In this work, first, we determined the main shock epicenter using the accelerograms recorded by the Algerian accelerograph network (under the coordination of the National Center of Applied Research in Earthquake Engineering-CGS). We calculated the focal mechanism of the main shock, using the inversion of the accelerograph waveforms in displacement that provides a reverse fault with a slight right-lateral component of slip and a compression axis striking NNW-SSE. The obtained scalar seismic moment (M sub(o) = 1.25 10 super(17) Nm) corresponds to a moment magnitude of M sub(w) = 5.3. Second, the analysis of the obtained aftershock swarm, of the survey, suggests an offshore ENE-WSW, trending and NNW dipping, causative active fault in the bay of Algiers, which may likely correspond to an offshore unknown segment of the Sahel active fault.
We report the case of a 14-year-old boy who underwent an aortobi-iliac bypass with a femoral vein graft. The patient presented with early aneurysmal degeneration of the entire venous graft only ...11 months later. He was treated successfully with redo abdominal aortic surgery and a bifurcated Dacron graft.
Nerve fibrolipoma is a very rare benign tumor, corresponding to fibrofatty proliferation of the epineurium and perineurium, where most frequently, the median nerve is touched. We report the case of a ...52-year-old patient who has a giant fibrolipoma of the thenar branch of the median nerve evolving at the level of the thenar eminence, an exceptional size and location for this disorder. Our case presents fibrolipoma of the median nerve in thenar eminence, a size and localization that has not been reported in the literature to our knowledge A good dissection makes it possible to obtain total resection without neurological after-effects.
Background: Giant cell tumors (GCTs) are locally aggressive but rarely malignant bone neoplasms that uncommonly involve the skull. In this report, we describe a tumor of the sphenoid sinus. Case ...presentation: A 51-year-old female was presented with headache, and bilateral decreased visual acuity, CT scan, and brain MRI revealed an infra-sellar enhancing tumor expanding to the sellar and supra-sellar region which proved to be a GCT. the patient had received 03 months of preoperative denosumab-based treatment and imaging follow-up showed regression in size and morphology modifications of tumor tissue. Conclusion: This is one of few reports to describe the appearance of sphenoid bone GCT, and the first report to highlight the effects of short-term denosumab treatment in GCTb in such a location.
We present an algorithm that computes an unmixed-dimensional decomposition of a finitely generated perfect differential ideal I. Each Iiin the decompositionI=I1∩⋯ ∩Ikis given by its characteristic ...set. This decomposition is a generalization of the differential case of Kalkbrener’s decomposition. We use a different approach. The basic operation in our algorithm is the computation of the inverse of an algebraic polynomial with respect to a finite set of algebraic polynomials. No factorization is needed. Some of the main problems in polynomial ideal theory can be solved by means of this decomposition: we show how the radical membership can be decided, a characteristic set of a prime differential ideal can be selected, and the differential dimension with a parametric set of a differential ideal can be read. The algorithm has been implemented in the computer algebra system MAPLE and has been tested successfully on many examples.
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