This paper forms part of a project to attempt to understand what we are really doing when we engage in the practices of public archaeology and heritage. It starts from the premise that there is no ...necessary correlation between intention and outcome, nor that practice follows belief. Instead, we construct our ideas about the world, about ourselves, our values and associations from what we do. The stories told here raise issues about the ability of archaeology to change the world and the distinction that we can too easily blur between professional expertise and citizenship.
In vitro zygotic and somatic embryogenesis protocols rely on nutrient and hormone levels from media to satisfy the physiological and developmental requirements of embryony. To better understand these ...requirements for cotton, we quantified levels of major and minor elements, carbohydrates, NH₄ ⁺, free amino acids and six hormones in whole cotton ovules (with fibers removed), nucelli (ovules with integuments removed), or ovule fluid (extracted from the endosperm region). Samples were collected from field-grown cotton at 1-18 days-past-anthesis (DPA) during each of three growing seasons. Replication across 2 years was obtained for carbohydrates, NH₄ ⁺, free amino acids and hormones from nucellus samples. The year effect was large primarily for hormones only. The most abundant minerals across tissue types and years were K, P, Mg and S. Potassium was the most abundant at 260, 600 and 1,660 mmol kg⁻¹ dry mass (DM) in nucelli, whole ovules and ovule fluid, respectively. Magnesium, Ca, Zn and Mn levels were 2-8-fold higher in ovule fluid compared to whole ovules or nucelli. In the free amino acid plus NH₄ ⁺ category, NH₄ ⁺, alanine, serine, glycine, asparagine (plus aspartic acid), glutamine (plus glutamic acid), leucine, threonine and arginine predominated in nucelli and ovule fluid, and levels tended to be higher in the older samples across years and tissue types. Fructose and glucose levels also increased with age with very high levels being found in late DPA ovule fluid. Arabinose, inositol and melibiose were also prominent sugars. Indole-3-acetic acid levels were similar between nucelli and ovule fluid and ranged from 10 to 80 μmol kg⁻¹ DM. An abscisic acid spike, from 15 to 400 μmol kg⁻¹ DM, occurred in nucelli and whole ovules from 2 to 8 DPA. Thereafter, abscisic acid levels remained between 5 and 10 μmol kg⁻¹ DM. Zeatin and zeatin riboside were the most abundant cytokinins, and levels of these hormones fluctuated between 1 and 4 μmol kg⁻¹ DM in both nucelli and ovule fluid.
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