Sepsis is a systemic inflammatory response syndrome caused by infection, resulting in organ dysfunction. Sepsis‐induced acute kidney injury (AKI) is one of the most common potential complications. ...Increasing reports have shown that M1 and M2 macrophages both take part in the progress of AKI by influencing the level of inflammatory factors and the cell death, including pyroptosis. However, whether M1 and M2 macrophages regulate AKI by secreting exosome remains unknown. In the present study, we isolated the exosomes from M1 and M2 macrophages and used Western blot and enzyme‐linked immunosorbent assay (ELISA) to investigate the effect of M1 and M2 exosomes on cell pyroptosis. miRNA sequencing was used to identify the different miRNA in M1 and M2 exosomes. Luciferase reporter assay was used to verify the target gene of miRNA. We confirmed that exosomes excreted by macrophages regulated cell pyroptosis in vitro by using Western blot and ELISA. miRNA sequencing revealed the differentially expressed level of miRNAs in M1 and M2 exosomes, among which miR‐93‐5p was involved in the regulation of pyroptosis. By using bioinformatics predictions and luciferase reporter assay, we found that thioredoxin–interacting protein (TXNIP) was a direct target of miR‐93‐5p. Further in vitro and in vivo experiments indicated that exosomal miR‐93‐5p regulated the TXNIP directly to influence the pyroptosis in renal epithelial cells, which explained the functional difference between different phenotypes of macrophages. This study might provide new targets for the treatment of sepsis‐induced AKI.
The antiferromagnetic spin-1/2 Heisenberg model on a kagome lattice is one of the most paradigmatic models in the context of spin liquids, yet the precise nature of its ground state is not ...understood. We use large-scale density matrix renormalization group simulations (DMRG) on infinitely long cylinders and find indications for the formation of a gapless Dirac spin liquid. First, we use adiabatic flux insertion to demonstrate that the spin gap is much smaller than estimated from previous DMRG simulation. Second, we find that the momentum-dependent excitation spectrum, as extracted from the DMRG transfer matrix, exhibits Dirac cones that match those of a π -flux free-fermion model the parton mean-field ansatz of a U(1) Dirac spin liquid.
Quantum magnets provide the simplest example of strongly interacting quantum matter, yet they continue to resist a comprehensive understanding above one spatial dimension. We explore a promising ...framework in two dimensions, the Dirac spin liquid (DSL) - quantum electrodynamics (QED
) with 4 Dirac fermions coupled to photons. Importantly, its excitations include magnetic monopoles that drive confinement. We address previously open key questions - the symmetry actions on monopoles on square, honeycomb, triangular and kagome lattices. The stability of the DSL is enhanced on triangular and kagome lattices compared to bipartite (square and honeycomb) lattices. We obtain the universal signatures of the DSL on triangular and kagome lattices, including those of monopole excitations, as a guide to numerics and experiments on existing materials. Even when unstable, the DSL helps unify and organize the plethora of ordered phases in correlated two-dimensional materials.
Tumor metastasis is a hallmark of cancer. The communication between cancer-derived exosomes and stroma plays an irreplaceable role in facilitating pre-metastatic niche formation and cancer ...metastasis. However, the mechanisms underlying exosome-mediated pre-metastatic niche formation during colorectal cancer (CRC) liver metastasis remain incompletely understood. Here we identified HSPC111 was the leading upregulated gene in hepatic stellate cells (HSCs) incubated with CRC cell-derived exosomes. In xenograft mouse model, CRC cell-derived exosomal HSPC111 facilitated pre-metastatic niche formation and CRC liver metastases (CRLM). Consistently, CRC patients with liver metastasis had higher level of HSPC111 in serum exosomes, primary tumors and cancer-associated fibroblasts (CAFs) in liver metastasis than those without. Mechanistically, HSPC111 altered lipid metabolism of CAFs by phosphorylating ATP-citrate lyase (ACLY), which upregulated the level of acetyl-CoA. The accumulation of acetyl-CoA further promoted CXCL5 expression and secretion by increasing H3K27 acetylation in CAFs. Moreover, CXCL5-CXCR2 axis reinforced exosomal HSPC111 excretion from CRC cells and promoted liver metastasis. These results uncovered that CRC cell-derived exosomal HSPC111 promotes pre-metastatic niche formation and CRLM via reprogramming lipid metabolism in CAFs, and implicate HSPC111 may be a potential therapeutic target for preventing CRLM.
Microalgal lipid is one of the most promising feedstocks for biodiesel production. Chlorella appears to be a particularly good option, and nitrogen (N) starvation is an efficient environmental ...pressure used to increase lipid accumulation in Chlorella cells. The effects of N starvation of an oil-producing wild microalga, Chlorella sorokiniana C3, on lipid accumulation were investigated using thin layer chromatography (TLC), confocal laser scanning microscopy (CLSM) and flow cytometry (FCM). The results showed that N starvation resulted in lipid accumulation in C. sorokiniana C3 cells, oil droplet (OD) formation and significant lipid accumulation in cells were detected after 2 d and 8 d of N starvation, respectively. During OD formation, reduced photosynthetic rate, respiration rate and photochemistry efficiency accompanied by increased damage to PSII were observed, demonstrated by chlorophyll (Chl) fluorescence, 77K fluorescence and oxygen evolution tests. In the mean time the rate of cyclic electron transportation increased correspondingly to produce more ATP for triacylglycerols (TAGs) synthesis. And 0.5 d was found to be the turning point for the early stress response and acclimation of cells to N starvation. Increased level of membrane peroxidation was also observed during OD formation, and superoxide dismutase (SOD), peroxide dismutase (POD) and catalase (CAT) enzyme activity assays suggested impaired reactive oxygen species (ROS) scavenging ability. Significant neutral lipid accumulation was also observed by artificial oxidative stress induced by H2O2 treatment. These results suggested coupled neutral lipid accumulation and oxidative stress during N starvation in C. sorokiniana C3.
In smart grid, residential consumers adopt different load scheduling methods to manage their power consumptions with specific objectives. The conventional load scheduling methods aim to maximize the ...consumption payoff or minimize the consumption cost. In this paper, we introduce a novel concept of cost efficiency-based residential load scheduling framework to improve the economical efficiency of the residential electricity consumption. The cost efficiency is defined as the ratio of consumer's total consumption benefit to its total electricity payment during a certain period. We develop a cost-efficient load scheduling algorithm for the demand-side's day-ahead bidding process and real-time pricing mechanism by using a fractional programing approach. Results show that the proposed scheduling algorithm can effectively reflect and affect user's consumption behavior and achieve the optimal cost-efficient energy consumption profile. For practical consideration, we also take into account the service fee and distributed energy resources (DERs) in our framework, and analyze their impacts on the cost efficiency. Simulation results confirm that the proposed algorithm significantly improves consumer's cost efficiency. It is shown that a higher service fee will decrease the cost efficiency, while the integration of DERs can effectively improve the cost efficiency.
Respiratory tract viral infection caused by viruses or bacteria is one of the most common diseases in human worldwide, while those caused by emerging viruses, such as the novel coronavirus, 2019‐nCoV ...that caused the pneumonia outbreak in Wuhan, China most recently, have posed great threats to global public health. Identification of the causative viral pathogens of respiratory tract viral infections is important to select an appropriate treatment, save people's lives, stop the epidemics, and avoid unnecessary use of antibiotics. Conventional diagnostic tests, such as the assays for rapid detection of antiviral antibodies or viral antigens, are widely used in many clinical laboratories. With the development of modern technologies, new diagnostic strategies, including multiplex nucleic acid amplification and microarray‐based assays, are emerging. This review summarizes currently available and novel emerging diagnostic methods for the detection of common respiratory viruses, such as influenza virus, human respiratory syncytial virus, coronavirus, human adenovirus, and human rhinovirus. Multiplex assays for simultaneous detection of multiple respiratory viruses are also described. It is anticipated that such data will assist researchers and clinicians to develop appropriate diagnostic strategies for timely and effective detection of respiratory virus infections.
Research Highlights
Respiratory tract viral infection including 2019‐nCoV poses great threats worldwide. Currently available and novel emerging diagnostic methods are summarized for several common respiratory viruses, including influenza virus, human respiratory syncytial virus, coronavirus, human adenovirus and human rhinovirus. Multiplex assays for simultaneous detection of multiple respiratory viruses are also described. This review is aimed to assist researchers and clinicians to develop timely and effective diagnostic strategies to detect respiratory virus infections.
Bronchopulmonary dysplasia (BPD) is a severe complication of preterm infants characterized by increased alveolarization and inflammation. Premature exposure to hyperoxia is believed to be a key ...contributor to the pathogenesis of BPD. No effective preventive or therapeutic agents have been created. Stimulator of interferon gene (STING) is associated with inflammation and apoptosis in various lung diseases. Long non‐coding RNA MALAT1 has been reported to be involved in BPD. However, how MALAT1 regulates STING expression remains unknown. In this study, we assessed that STING and MALAT1 were up‐regulated in the lung tissue from BPD neonates, hyperoxia‐based rat models and lung epithelial cell lines. Then, using the flow cytometry and cell proliferation assay, we found that down‐regulating of STING or MALAT1 inhibited the apoptosis and promoted the proliferation of hyperoxia‐treated cells. Subsequently, qRT‐PCR, Western blotting and dual‐luciferase reporter assays showed that suppressing MALAT1 decreased the expression and promoter activity of STING. Moreover, transcription factor CREB showed its regulatory role in the transcription of STING via a chromatin immunoprecipitation. In conclusion, MALAT1 interacts with CREB to regulate STING transcription in BPD neonates. STING, CREB and MALAT1 may be promising therapeutic targets in the prevention and treatment of BPD.
Evidence indicates that downloading on-demand videos accounts for a dramatic increase in data traffic over cellular networks. Caching popular videos in the storage of small-cell base stations (SBS), ...namely, small-cell caching, is an efficient technology for reducing the transmission latency while mitigating the redundant transmissions of popular videos over back-haul channels. In this paper, we consider a commercialized small-cell caching system consisting of a network service provider (NSP), several video retailers (VRs), and mobile users (MUs). The NSP leases its SBSs to the VRs for the purpose of making profits, and the VRs, after storing popular videos in the rented SBSs, can provide faster local video transmissions to the MUs, thereby gaining more profits. We conceive this system within the framework of Stackelberg game by treating the SBSs as specific types of resources. We first model the MUs and SBSs as two independent Poisson point processes, and develop, via stochastic geometry theory, the probability of the specific event that an MU obtains the video of its choice directly from the memory of an SBS. Then, based on the probability derived, we formulate a Stackelberg game to jointly maximize the average profit of both the NSP and the VRs. In addition, we investigate the Stackelberg equilibrium by solving a non-convex optimization problem. With the aid of this game theoretic framework, we shed light on the relationship between four important factors: the optimal pricing of leasing an SBS, the SBSs allocation among the VRs, the storage size of the SBSs, and the popularity distribution of the VRs. Monte Carlo simulations show that our stochastic geometry-based analytical results closely match the empirical ones. Numerical results are also provided for quantifying the proposed game-theoretic framework by showing its efficiency on pricing and resource allocation.
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