Abstract
Pre-synchronization strategies with prolonged exposure to exogenous progesterone have the potential to alter estrus expression and improve fertility in replacement beef heifers. The ...objective of this study was to evaluate the estrus response and pregnancy rate of beef heifers enrolled in two fixed-time artificial insemination (TAI) protocols. A total of 306 heifers (14 ± 2 months of age; 372 ± 24 kg body weight BW) at three locations were enrolled in the study. Within location, heifers were stratified by age and BW and randomly assigned to one of two treatments: 1) PG-CIDR54 (n = 152), heifers were administered prostaglandin F2α (PGF; 25 mg) and a controlled internal drug releasing (CIDR) insert (Day -14), an injection of GnRH 100 μg (Day -7), PGF injection and CIDR removal (Day 0), and a second injection of GnRH concurrently with TAI 54 ± 2 h later (Day 2); 2) 7-day CO-Synch+CIDR (CTRL; n = 154), heifers were administered GnRH (100 μg) and a CIDR insert (Day -7), PGF (25 mg) injection and CIDR removal (Day 0), and a second injection of GnRH concurrently with TAI 54 ± 2 h later (Day 2). All heifers received an estrus alert patch on Day 0 and were evaluated for patch activation at TAI on Day 2. Pregnancy was diagnosed by transrectal ultrasonography between 35 and 55 days after TAI. The percentage of heifers exhibiting estrus between Day 0 and 2 was greater (P < 0.01) in PG-CIDR54 compared with CTRL (77.0 and 39.6%, respectively). In addition, pregnancy rate to TAI tended (P = 0.08) to be greater for PG-CIDR54 compared with CTRL (48.0 and 38.3%, respectively). We conclude that beef heifers enrolled in the PG-CIDR54 protocol had increased estrus expression and tended to have greater pregnancy rate when compared with heifers enrolled in the 7-day CO-Synch+CIDR protocol.
Abstract Objective To provide a user's guide for selecting an appropriate method to assess sedentary behaviours among children and adolescents. Design While recommendations regarding specific ...instruments are not provided, the guide offers information about key attributes and considerations for objective (accelerometry; inclinometers; direct observation; screen monitoring devices) and subjective (self-report; parent report; and time use diaries/logs) approaches to assess sedentary behaviour Attributes of instruments and other factors to be considered in the selection of assessment instruments include: population (age); sample size; respondent burden; method/delivery mode; assessment time frame; physical activity information required (data output); data management; measurement error; cost (instrument and administration) and other limitations. Methods Expert consensus among members of the Australasian Child and Adolescent Obesity Research Network's (ACAORN) Physical Activity and Sedentary Behaviour Special Interest Group. Results We developed decision flow charts to assist researchers and practitioners select an appropriate method of assessing sedentary behaviour, identified attributes of each method and described five real-life scenarios to illustrate considerations associated with the selection of each method of measurement. Conclusions It is important that researchers, practitioners and policy makers understand the strengths and limitations of different methods of assessing sedentary behaviour among youth, and are guided on selection of the most appropriate instrument/s to suit their needs.
Desulfovibrio carbinoliphilus subsp. oakridgensis FW-101-2B is an anaerobic, organic acid/alcohol-oxidizing, sulfate-reducing δ-proteobacterium. FW-101-2B was isolated from contaminated groundwater ...at The Field Research Center at Oak Ridge National Lab after in situ stimulation for heavy metal-reducing conditions. The genome will help elucidate the metabolic potential of sulfate-reducing bacteria during uranium reduction.
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