Introdução: O tumor de células granulares (TCG) é uma neoplasia rara e predominantemente benigna, frequentemente localizada na região da cabeça e pescoço. Comumente, afeta adultos entre 40 e 60 anos, ...com maior incidência em mulheres. O TCG mamário, representando de 5% a 15% de todos os TCG, demanda diagnóstico histopatológico em virtude de semelhanças clínicas e radiológicas com malignidades mamárias. Tipicamente, manifesta-se como massa solitária palpável, embora a multicentricidade ocorra em até 20% dos casos, seja em tumores mamários ou de outros sítios. Relato do caso: Paciente, 43 anos, com nódulo hipoecoico classificado como BI-RADS 5 na ultrassonografia, localizado no quadrante superolateral da mama esquerda, às duas horas, de formato irregular, medindo 7,0 x 6,0 mm nos maiores eixos e distando 50 mm da papila. Por ser radiologicamente suspeito, foi realizada a core biopsy mamária, que microscopicamente foi categorizada como lesão de potencial maligno incerto. A análise imuno-histoquímica revelou positividade para S100 e CD68, indicando origem em células de Schwann, associada à negatividade para citoqueratinas e receptores hormonais, desfavorecendo a origem epitelial dos carcinomas. A conduta terapêutica foi a exérese cirúrgica completa do nódulo, e a análise histopatológica confirmou tratar-se de TCG. Conclusão: Relatar essa neoplasia faz-se importante em razão da raridade da condição e sua capacidade de mimetizar cânceres mamários.
Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease of the motor neuron for which no clinically validated biomarkers have been identified.
We have quantified by ELISA the ...biomarker phosphoneurofilament heavy chain (pNFH) in the cerebrospinal fluid (CSF) of ALS patients (n=29) and age-matched control patients with other diseases (n=19) by ELISA. Furthermore, we compared protein N-glycosylation of the CSF in ALS patients and controls, by applying a glycomics approach based on liquid chromatography and mass spectrometry.
pNFH levels were significantly higher in ALS patients in comparison with controls (P<0.0001) in particular in fast progressors. The N-glycans found in the CSF were predominantly complex diantennary with sialic acid in α2,3- and α2,6-linkage, and bisecting N-acetylglucosamine-containing structures as well as peripherally fucosylated structures were found. As compared with controls the ALS group had a significant increase of a peak composed of the monosialylated diantennary glycans A2G2S(6)1 and FA2G2S(3)1 (P=0.0348).
Our results underscore the value of pNFH as a biomarker in ALS. In addition, we identified a variation of the N-glycosylation pattern in ALS, suggesting that this change should be explored in future studies as potential biomarker.
•pNFH levels were significantly higher in ALS CSF in comparison with controls.•CSF N-glycans were mostly of the complex type and “brain-type” glycans were found.•Specific monosialylated diantennary N-glycans were significantly higher in ALS CSF.
OBJECTIVE: Neurofilaments are leading neurochemical biomarkers for amyotrophic lateral sclerosis (ALS). Here, we investigated the effect of preanalytical factors on neurofilament concentrations in ...cerebrospinal fluid (CSF) in a "reverse" round-robin with 15 centers across Europe/U.S. METHODS: Samples from ALS and control patients (5/5 each center, n = 150) were analyzed for phosphorylated neurofilament heavy chain (pNfH) and neurofilament light chain (NfL) at two laboratories. RESULTS: CSF pNfH was increased (p < 0.05) in ALS in 10 out of 15 centers and NfL in 5 out of 12 centers. The coefficient of variation (CV%) of pNfH measurements between laboratories was 18.7 ± 19.1%. We calculated a diagnostic cut-off of >568.5 pg/mL for pNfH (sensitivity 78.7%, specificity 93.3%) and >1,431pg/mL for NfL (sensitivity 79.0%, specificity 86.4%). CONCLUSION: Values in ALS patients are already comparable between most centers, supporting eventual implementation into clinical routine. However, continuous quality control programs will be necessary for inclusion in the diagnostic work-up.
A disintegrin and metalloprotease 10 (ADAM10) is a type I transmembrane glycoprotein with four potential
N-glycosylation sites (N267, N278, N439 and N551), that cleaves several plasma membrane ...proteins. In this work, ADAM10 was found to contain high-mannose and complex-type glycans. Individual
N-glycosylation site mutants S269A, T280A, S441A, T553A were constructed, and results indicated that all sites were occupied. T280A was found to accumulate in the endoplasmic reticulum as the non-processed precursor of the enzyme. Furthermore, it exhibited only residual levels of metalloprotease activity
in vivo towards the L1 cell adhesion molecule, as well as
in vitro, using a ProTNF-alpha peptide as substrate. S441A showed increased ADAM10 susceptibility to proteolysis. Mutation of N267, N439 and N551 did not completely abolish enzyme activity, however, reduced levels were found. ADAM10 is sorted into secretory vesicles, the exosomes. Here, a fraction of ADAM10 from exosomes was found to contain more processed
N-linked glycans than the cellular enzyme. In conclusion,
N-glycosylation is crucial for ADAM10 processing and resistance to proteolysis, and results suggest that it is required for full-enzyme activity.
This handbook presents a comprehensive, concise and accessible overview of the field of Historical International Relations (HIR). It summarizes and synthesizes existing contributions to the field ...while presenting central themes, approaches and methodologies that have driven the development of HIR, providing the reader with a sense of the diversity and research dynamics that are at the heart of this field of study. The wide range of topics covered are grouped under the following headings:
Traditions: Demonstrates the wide variety of approaches to HIR.
Thinking International Relations Historically: Different ways of thinking IR historically share some common concerns and areas for further investigation.
Actors, Processes and Institutions: Explores the processes, actors, practices, and institutions that constitute the core objects of study of many HIR scholars.
Situating Historical International Relations: Critically reflects about the situatedness of our objects of study.
Approaches: Examines how HIR scholars conduct and reflect about their research, often in dialogue with a variety of perspectives from cognate disciplines.
Summarizing key contributions and trends while also sketching out challenges for future inquiry, this is an invaluable resource for students, academics and researchers from a range of disciplines, particularly International Relations, global history, political science, history, sociology, anthropology, peace studies, diplomatic studies, security studies, international political thought, political geography, international law.
Cerebrospinal fluid (CSF) is a clear, transparent fluid derived from blood plasma that protects the brain and spinal cord against mechanical shock, provides buoyancy, clears metabolic waste and ...transports extracellular components to remote sites in the brain. Given its contact with the brain and the spinal cord, CSF is the most informative biofluid for studies of the central nervous system (CNS). In addition to other components, CSF contains extracellular vesicles (EVs) that carry bioactive cargoes (e.g., lipids, nucleic acids, proteins), and that can have biological functions within and beyond the CNS. Thus, CSF EVs likely serve as both mediators of and contributors to communication in the CNS. Accordingly, their potential as biomarkers for CNS diseases has stimulated much excitement for and attention to CSF EV research. However, studies on CSF EVs present unique challenges relative to EV studies in other biofluids, including the invasive nature of CSF collection, limited CSF volumes and the low numbers of EVs in CSF as compared to plasma. Here, the objectives of the International Society for Extracellular Vesicles CSF Task Force are to promote the reproducibility of CSF EV studies by providing current reporting and best practices, and recommendations and reporting guidelines, for CSF EV studies. To accomplish this, we created and distributed a world‐wide survey to ISEV members to assess methods considered ‘best practices’ for CSF EVs, then performed a detailed literature review for CSF EV publications that was used to curate methods and resources. Based on responses to the survey and curated information from publications, the CSF Task Force herein provides recommendations and reporting guidelines to promote the reproducibility of CSF EV studies in seven domains: (i) CSF Collection, Processing, and Storage; (ii) CSF EV Separation/Concentration; (iii) CSF EV Size and Number Measurements; (iv) CSF EV Protein Studies; (v) CSF EV RNA Studies; (vi) CSF EV Omics Studies and (vii) CSF EV Functional Studies.
ABSTRACT This article aims to analyze, from a Bakhtinian perspective, the grotesque construction of Mexican artist Frida Kahlo’s body through a religious worldview. Therefore, we analyze two of her ...self-portraits, taking into account the dialogic relations constructed between the grotesque representations and Catholic symbology. The analyzed utterances, in which the specificities of the creative competence of the author-creator in her aesthetic activity are inferred, showed that a dialogue is established between the painting El venado herido and Catholic Saint Sebastian and between the painting Las dos fridas and the image of the Sacred Heart of Jesus.
RESUMO O presente artigo tem como objetivo analisar, sob a perspectiva bakhtiniana, a construção grotesca do corpo fridiano por meio de uma cosmovisão religiosa. Para tanto, são analisados dois autorretratos produzidos pela pintora mexicana Frida Kahlo, levando em consideração as relações dialógicas construídas entre as representações grotescas e as simbologias católicas. Nos enunciados analisados, nos quais são depreendidas as especificidades da competência criativa da autora-criadora em sua atividade estética, percebe-se o correlacionamento entre a pintura El venado herido e a narrativa do santo católico São Sebastião e o diálogo entre a tela Las dos fridas e a imagem do Sagrado Coração de Jesus.
To evaluate the correlation between religiosity, spirituality, and self-esteem in adolescents with uni- and bilateral cleft lip and palate.
Correlational and cross-sectional study developed in a ...public and tertiary hospital in Brazil between July 2018 and February 2019. The sample comprised 100 adolescents divided into two groups: G1 (unilateral cleft, 50 participants) and G2 (bilateral cleft, 50 participants). For data collection, three instruments were used: Sociodemographic Questionnaire, DUREL Religion index, Rosenberg self-esteem scale. Statistical analysis was performed with the tests Chi-squared, Mann-Whitney, Pearson Correlation, and analysis of linear correlation strength, with a 5% significance level (p ≥ 0.05).
Only organizational religiosity was higher in G1 when compared with G2 (p = 0.03). The overall self-esteem was satisfactory for both groups; however, there was no significant difference between them (p = 0.34). No correlation between religiosity and spirituality with self-esteem were identified for G1 and G2.
The adolescents with uni- or bilateral cleft lip and palate presented high levels of religiosity, spirituality, and self-esteem. However, no correlation between these variables was identified.
Ovarian carcinoma is the leading cause of death from gynecological cancers in many Western countries. Aberrant glycosylation is an important aspect in malignant transformation and consequently in ...ovarian cancer. In this study, a detailed structure analysis of the N-linked glycans from total glycoproteins from the SKOV3 ovarian carcinoma cell line and from a recombinantly expressed secretory glycoprotein, erythropoietin (EPO), produced from the same cells has been performed using high-performance anion exchange chromatography with pulsed amperometric detection and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Total cellular N-glycans contained high-mannose type and proximally fucosylated complex type partially agalactosylated structures. On the other hand, the recombinant human EPO secreted from SKOV3 cells contained predominantly core-fucosylated tetraantennary structures, which were partially lacking one or two galactose residues, and partially contained the LacdiNAc motif. Only minor amounts of di- and triantennary complex-type glycans were found, and high-mannose-type glycans were not present in the secreted EPO protein. A large amount of N-acetylneuraminic acid in α2,3-linkage was detected as well. Endogenous glycoproteins were also found to contain the LacdiNAc motif in N-linked glycans. This work contributes to the knowledge of the glycosylation of a human ovarian cancer cell line. It also establishes the basis to further explore high-mannose-type glycans, and the LacdiNAc motif as possible markers of ovarian carcinoma.
Sarcocystidae in wild birds of southeastern Brazil Rêgo, Wagner Martins Fontes do; Costa, Júlia Gatti Ladeia; Baraviera, Ramon Castro de Araujo ...
Revista brasileira de parasitologia veterinaria,
01/2021, Letnik:
30, Številka:
1
Journal Article
Recenzirano
Odprti dostop
This study aimed to identify members of the Sarcocystidae family in naturally infected wild birds at a rescue center in the state of Minas Gerais, southeastern Brazil. The heart and brain of 44 wild ...birds were evaluated by bioassay in mice to detect T. gondii, and extracted DNA was used for nested PCR of the 18S ribosomal DNA gene to detect members of the Sarcocystidae family. The positive samples were sequenced, assembled, edited and compared with sequences deposited in GenBank. Toxoplasma gondii was isolated from six (13.6%) out of 44 birds. Toxoplasma gondii DNA was identified in 10/44 (22.7%) of the birds. The amplified sequences exhibited 100% similarity with the DNA of the ME49 strain of T. gondii. Sarcocystis DNA (99% similarity) was identified in 5/44 (11.4%) of the birds. T. gondii and Sarcocystis spp. are common in wild birds in Minas Gerais, Brazil.
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