is a heterotrophic marine microalga that has potential industrial applications. The main objectives of this study were to isolate an
strain from Sand Cay (Son Ca) Island, Vietnam, optimize its ...culture conditions, determine its nutritional composition, extract polyunsaturated fatty acids (PUFAs) in the free (FFA) and the alkyl ester (FAAE) forms, and evaluate the antioxidation and neuroprotection properties of the PUFAs.
sp. SC145 can be grown stably under laboratory conditions. Its culture conditions were optimized for a dry cell weight (DCW) of 31.18 g/L, with total lipids comprising 25.29%, proteins 7.93%, carbohydrates 15.21%, and carotenoid at 143.67 µg/L of DCW. The FAAEs and FFAs extracted from
sp. SC145 were rich in omega 3-6-9 fatty acids (40.73% and 44.00% of total fatty acids, respectively). No acute or subchronic oral toxicity was determined in mice fed with the PUFAs in FFA or FAAE forms at different doses over 90 days. Furthermore, the PUFAs in the FFA or FAAE forms and their main constituents of EPA, DHA, and ALA showed antioxidant and AChE inhibitory properties and neuroprotective activities against damage caused by H
O
- and amyloid-ß protein fragment 25-35 (Aβ
)-induced C6 cells. These data suggest that PUFAs extracted from
sp. SC145 may be a potential therapeutic target for the treatment of neurodegenerative disorders.
Halophilic bacteria are receiving increasing attention for industrial chemical production processes due to their unique properties. Herein, an alkaliphilic and halophilic bacterium was isolated from ...a commercial
Spirulina
culture at Nghe An province in Vietnam and found to secrete pyruvate. Pyruvate is widely used as a starting material in the industrial biosynthesis of pharmaceuticals, and is employed for production of crop protection agents, polymers, cosmetics, and food additives. Phenotypic and chemotaxonomic characterization, and the 16S rRNA gene sequence homology with
Halomonas hydrothermalis
strain DSM 15,725 (99.2%) predicted that the strain belongs to the
Halomonas
genus, thus we named this strain as
H. hydrothermalis
strain C22. We investigated the biocharacteristics and capacity of strain C22 and determined the draft genome sequence comprising 3,934,166 bp with a G + C content of 60.2% encoding 3,668 proteins, 58 tRNAs, 9 rRNAs, and 1 tmRNA. Maximal pyruvate secretion reached 51.1 g/l after 84 h of cultivation. The results will facilitate future studies on the genetic and metabolic diversity of halophilic bacteria and expand our understanding of important bioprocesses in this microorganism.
Information sharing plays a key role in supply chain performance. According to previous individual studies, technology, trust, commitment, and uncertainty are four potential factors affecting ...information sharing. However, most studies focus on testing a positive relationship between each factor and information sharing. Therefore, it is necessary to evaluate the effect of each factor on information sharing.
To determine the accurate effect of factors on sharing information and propose key factors to support decision-makers in improving their information sharing.
Correlation coefficients between factors are collected from 41 individual studies with a total of 8,983 samples on Google Scholar.
Using the rank correlation test and Egger's regression test to test publication bias. The meta-analysis method is used to perform analysis models, including fixed-effect, random-effect, and Hunter and Schmidt methods.
Commitment plays the most important role in information sharing when compared to technology, trust, and uncertainty. Commitment accounts for 19% in the Hunter and Schmidt method and 22% in both fixed-effect and random-effect models. In addition, the result indicates that there are around 50% of other factors that affect the efficiency of sharing information besides four factors in our studies.
Technology, trust, and commitment significantly affect information sharing, of which the impact of commitment on information sharing is strongest and should be a priority in improving the effectiveness of information sharing. Our study contributes two findings to literature in the field of supply chain information sharing: 1) certain confirming the important role of commitment on sharing information, and 2) the necessity of considering other factors besides these four elements.
The question of regional per-capita income convergence has been widely investigated theoretically and empirically. This study reviews the relevant literature and considers the possible significance ...of fiscal decentralisation in the convergence process. Alternative indicators of fiscal decentralization are examined within a SDM model which also takes into account possible spatial interdependencies between neighbouring regions. The modelling suggests that fiscal decentralization does appear to play a significant role in the convergence process in Vietnam. Income inequality remains a challenging issue for policymakers to resolve, and if regional disparities can be reduced in part through fiscal decentralization, this is a significant finding for policy formulation.
Shigella sonnei is a pathogen of growing global importance as a cause of diarrhoeal illness in childhood, particularly in transitional low-middle income countries (LMICs). Here, we sought to ...determine the incidence of childhood exposure to S. sonnei infection in a contemporary transitional LMIC population, where it represents the dominant Shigella species.
Participants were enrolled between the age of 12-36 months between June and December 2014. Baseline characteristics were obtained through standardized electronic questionnaires, and serum samples were collected at 6-month intervals over two years of follow-up. IgG antibody against S. sonnei O-antigen (anti-O) was measured using an enzyme-linked immunosorbent assay (ELISA). A four-fold increase in ELISA units (EU) with convalescent IgG titre >10.3 EU was taken as evidence of seroconversion between timepoints.
A total of 3,498 serum samples were collected from 748 participants; 3,170 from the 634 participants that completed follow-up. Measures of anti-O IgG varied significantly by calendar month (p = 0.03). Estimated S. sonnei seroincidence was 21,451 infections per 100,000 population per year (95% CI 19,307-23,834), with peak incidence occurring at 12-18 months of age. Three baseline factors were independently associated with the likelihood of seroconversion; ever having breastfed (aOR 2.54, CI 1.22-5.26), history of prior hospital admission (aOR 0.57, CI 0.34-0.95), and use of a toilet spray-wash in the household (aOR 0.42, CI 0.20-0.89).
Incidence of S. sonnei exposure in Ho Chi Minh City is substantial, with significant reduction in the likelihood of exposure as age increases beyond 2 years.
In attempting to identify effective anticancer drugs from natural products that are harmless to humans, we found that the gomisin J from Schisandra chinensis fruit has anticancer activity. Schisandra ...chinensis fruits are used in traditional herbal medicine and gomisin J is one of their chemical constituents. In the present study, we examined the anticancer activity of gomisin J in MCF7 and MDA-MB-231 breast cancer cell lines and in MCF10A normal cell line, in a time- and concentration-dependent manner. Our data revealed that gomisin J exerted a much stronger cytotoxic effect on MCF7 and MDA-MB-231 cancer cells than on MCF10A normal cells. Gomisin J suppressed the proliferation and decreased the viability of MCF7 and MDA-MB-231 cells at relatively low (<10 µg/ml) and high (>30 µg/ml) concentrations, respectively. Our data also revealed that gomisin J induced necroptosis, a programmed form of necrosis, as well as apoptosis. Notably, gomisin J predominantly induced necroptosis in MCF7 cells that are known to have high resistance to many pro-apoptotic anticancer drugs, while MDA-MB-231 exhibited a much lower level of necroptosis but instead a higher level of apoptosis. This data indicated the possibility that it may be used as a more effective anticancer drug, especially in apoptosis-resistant malignant cancer cells. In an extended study, gomisin J exhibited a strong cytotoxic effect on all tested various types of 13 cancer cell lines, indicating its potential to be used against a wide range of different types of cancer cells.
Mice were used in in vivo experiments to evaluate the effects of doses of n-hexane extract (from 100 to 1,300 mg/kg body weight/day) on the ability to control obesity, blood glucose, and blood lipid. ...In this study, body weight gain, caloric intake, glucose tolerance, blood lipid, histopathological study, and locomotion activity were examined. Furthermore, this study evaluated the lethality of the extract in extremely high doses in the tested mice. After 3 months of use with an extremely high dose of 5,000 mg/kg body weight/day (equivalent to 350 g/day for a 70 kg person), no animals with abnormal conditions or death were observed. This initially demonstrated the safety of the extract. In addition, after 6 weeks of testing on high-fat diet-induced obese mice, n-hexane extract at a dose of 500 mg/kg body weight/day (equivalent to 35 g/day for a 70 kg person) demonstrated a positive effect on the ability to control obesity, blood glucose, and blood lipid through the results of body weight, blood lipids, glucose tolerance, and histopathology (white fat, liver, and kidney tissues). In this study, n-hexane extract from the roots of Duong-dau tree has proven to be strongly biologically active in preventing and supporting the treatment of diseases related to overweight and obesity, helping to control blood glucose levels thereby reducing the risk of type 2 diabetes.
The study was carried out to assess the pollution, impact, and risk level to the surface water environment of pollutants in the water bodies of Soc Trang province, Vietnam. The parameters for ...evaluating surface water quality and risks included temperature, pH, TSS, DO, BOD, COD, NH4+-N, NO2--N, NO3--N, PO43--P, Cl-, Fet, and coliform. Surface water samples were collected at 35 locations with a frequency of six times (February, April, June, August, October, and December) in 2022. The water quality index (WQI), impact and risk level (risk quotient or RQ, RQ-F), correlation analysis, and principal component analysis (PCA) were utilized in the study. The results show that the surface water has been seriously polluted due to organic matter, nutrients, microorganisms, iron, and salinity. The values of WQI in the dry and rainy seasons fluctuated between bad and very good, indicating that surface water quality is suitable for water transport and other purposes with higher quality requirements. TSS, COD, Fet and coliform have a high impact and risk for the environment in this study area. There were no environmental impacts and risks to NO3--N. Locations with many high-risk pollutants were mainly distributed in residential and coastal areas. The significant negative correlation between the WQI and RQ indicated that the lower the WQI, the higher the environmental risk. The PCA results show that at least six polluting sources affected water quality and caused environmental risks. The results of this study contribute essential and valuable information for improving water quality in the study area through the assessment of environmental impacts and risks. Doi: 10.28991/CEJ-2023-09-08-03 Full Text: PDF
Abstract
Mycobacterium tuberculosis
(Mtb) has different features depending on different geographic areas. We collected Mtb strains from patients with smear-positive pulmonary tuberculosis in Da Nang, ...central Vietnam. Using a whole genome sequencing platform, including genome assembly complemented by long-read-sequencing data, genomic characteristics were studied. Of 181 Mtb isolates, predominant Vietnamese EAI4_VNM and EAI4-like spoligotypes (31.5%), ZERO strains (5.0%), and part of EAI5 (11.1%) were included in a lineage-1 (L1) sublineage, i.e., L1.1.1.1. These strains were found less often in younger people, and they genetically clustered less frequently than other modern strains. Patients infected with ZERO strains demonstrated less lung infiltration. A region in RD2bcg spanning six loci, i.e.,
PE_PGRS35
,
cfp21
, Rv1985c, Rv1986, Rv1987, and
erm(37)
, was deleted in EAI4_VNM, EAI4-like, and ZERO strains, whereas another 118 bp deletion in
furA
was specific only to ZERO strains. L1.1.1.1-sublineage-specific deletions in
PE_PGRS4
and
PE_PGRS22
were also identified. RD900, seen in ancestral lineages, was present in majority of the L1 members. All strains without IS
6110
(5.0%) had the ZERO spoligo-pattern. Distinctive features of the ancestral L1 strains provide a basis for investigation of the modern versus ancestral Mtb lineages and allow consideration of countermeasures against this heterogeneous pathogen.
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