Fluorometric detection of O2 -• is performed based on desulfonylation of 3 to the corresponding fluoresceins 4 through nucleophilic substitution, and this fluorescing process is quite specific toward ...O2 -• over H2O2, t-BuOOH, NaOCl, 1O2, HO•, NO•, and ONOO-. Furthermore, effects of glutathione, cytochrome P450 reductase/NADPH, and diaphorase/NADH are relatively small on the fluorescing process of probe 3 with X = Y = F, which is useful to detect O2 -• released from neutrophils stimulated by phorbol myristate acetate with satisfactory sensitivity.
Highly selective detection of H2O2 through a non‐oxidative mechanism has been achieved by desulfonylation of 1 to the corresponding fluorescein. This approach has been used for the intracellular ...formation of H2O2 in algal cells by using the acetyl derivatives of 1 as fluorescent probes.
Die hochselektive Detektion von H2O2 beruht auf der Desulfonylierung von 1 zum Fluorescein‐Derivat nach einem nichtoxidativen Mechanismus. Mit dieser Methode wurde die Bildung von H2O2 in Algenzellen ...untersucht; dabei kamen Acetylderivate von 1 als Fluoreszenzsonden zum Einsatz.
HCO
secretion in distal airways is critical for airway mucosal defense. HCO
/H
transport across the apical membrane of airway surface epithelial cells was studied by measuring intracellular pH in ...luminally microperfused freshly dissected mice bronchioles. Functional studies demonstrated that CFTR, ENaC, Cl
-HCO
exchange, Na
-H
exchange, and Na
-HCO
cotransport are involved in apical HCO
/H
transport. RT-PCR of isolated bronchioles detected fragments from Cftr, α, β, γ subunits of ENaC, Ae2, Ae3, NBCe1, NBCe2, NBCn1, NDCBE, NBCn2, Nhe1, Nhe2, Nhe4, Nhe5, Slc26a4, Slc26a6, and Slc26a9. We assume that continuous decline of intracellular pH following alkaline load demonstrates time course of HCO
secretion into the lumen which is perfused with a HCO
-free solution. Forskolin-stimulated HCO
secretion was substantially inhibited by luminal application of CFTR
-172 (5 μM), H
DIDS (200 μM), and amiloride (1 μM). In bronchioles from a cystic fibrosis mouse model, basal and acetylcholine-stimulated HCO
secretion was substantially impaired, but forskolin transiently accelerated HCO
secretion of which the magnitude was comparable to wild-type bronchioles. In conclusion, we have characterized apical HCO
/H
transport in native bronchioles. We have demonstrated that cAMP-mediated and Ca
-mediated pathways are involved in HCO
secretion and that apical HCO
secretion is largely mediated by CFTR and H
DIDS-sensitive Cl
-HCO
exchanger, most likely Slc26a9. The impairment of HCO
secretion in bronchioles from a cystic fibrosis mouse model may be related to the pathogenesis of early lung disease in cystic fibrosis.
In recent years, local tourism has been gaining traction as one of the measures for regional revitalization in Japan. One of the organizations that support these are the sightseeing volunteer guide ...organizations. These organizations have various kinds of information that are helpful for local tourism and active information transmission is desirable. On the other hand, many organizations face the issue of the information transmission as their aging volunteer guides find it difficult to handle information devices. This study therefore aims to provide support for continuous information transmission by local sightseeing volunteer guides via social media, and attempts to clarify the transmission of sightseeing information that matches the characteristics of social media through proof-of-concept demonstrations with the Historical “CULTURAL PATH” Guide Volunteers in Nagoya City.
Cystic fibrosis (CF) is an autosomal recessive disease caused by pathogenic variants in CF transmembrane conductance regulator (CFTR). While CF is the most common hereditary disease in Caucasians, it ...is rare in East Asia. In the present study, we have examined clinical features and the spectrum of CFTR variants of CF patients in Japan. Clinical data of 132 CF patients were obtained from the national epidemiological survey since 1994 and CF registry. From 2007 to 2022, 46 patients with definite CF were analyzed for CFTR variants. All exons, their boundaries, and part of promoter region of CFTR were sequenced and the presence of large deletion and duplications were examined by multiplex ligation-dependent probe amplification. CF patients in Japan were found to have chronic sinopulmonary disease (85.6%), exocrine pancreatic insufficiency (66.7%), meconium ileus (35.6%), electrolyte imbalance (21.2%), CF-associated liver disease (14.4%), and CF-related diabetes (6.1%). The median survival age was 25.0 years. The mean BMI percentile was 30.3%ile in definite CF patients aged < 18 years whose CFTR genotypes were known. In 70 CF alleles of East Asia/Japan origin, CFTR-dele16-17a-17b was detected in 24 alleles, the other variants were novel or very rare, and no pathogenic variants were detected in 8 alleles. In 22 CF alleles of Europe origin, F508del was detected in 11 alleles. In summary, clinical phenotype of Japanese CF patients is similar to European patients, but the prognosis is worse. The spectrum of CFTR variants in Japanese CF alleles is entirely different from that in European CF alleles.
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