The aim of the present study was to perform a qualitative and quantitative analysis of the effect of different sucrose concentrations combined with ethylene glycol in the preservation of vitrified ...porcine preantral follicles. Fragments of ovarian cortex were vitrified in cryotubes containing 200 μl of the vitrification solution (30% Ethylene Glycol; 20% Fetal Bovine Serum; 0 M–0.25 M – 0.75 M or 1 M sucrose) and stored in liquid nitrogen for a week. Histological analysis showed that after vitrification the number of normal follicles decreased compared to the fresh tissue (control). The percentage of normal primordial follicles was sucrose dose dependent. The percentage of normal primary follicles was similar in 0 M or 0.25 M sucrose, while higher concentrations (0.75 M and 1 M) increased significantly the percentage of abnormal follicles (p < 0.05). Morphometric analysis showed a statistically significant reduction in the total area of primordial follicles with 0.75 M sucrose and a significant increase in the cytoplasmic area of primordial follicles with 0 M sucrose (p < 0.05). The qualitative and the quantitative analysis appear to be a complementary tool when choosing a vitrification protocol. For our cryopreservation system - vitrification of ovarian cortex slices in cryotubes-the best vitrification medium was TCM 199-Hepes with 30% de ethylene glycol, 20% of Fetal Bovine Serum and 0 or 0.25 M sucrose. The present study shows that the use of high sucrose concentrations in the vitrification solution has a deleterious effect on the preservation of porcine preantral follicles contained in ovarian tissue. Consequently, its use at 0.75 M or 1 M wouldn't be recommended.
•Primordial follicles appear to be more resistant to vitrification than primary.•High concentrations of sucrose seem to have a more deleterious effect.•The greatest damage occurred at the nucleus or at the nucleus/cytoplasm combined.•Quantitative analysis is a complementary tool to evaluate a vitrification protocol.•The best vitrification medium includes 30% ethylene glycol and 0 or 0.25 M sucrose.
The present research aims at exploring and deepening the knowledge about lobby and the influence power from pressure groups along to public agents in favour of the consumer. This qualitative work ...used a semi-structured interview as a data collecting tool. The results were processed and analysed through the content analysis technique, presented by analysis categories and subcategories. Based on the investigation, it was concluded that the pressure group and lobby activities presented themselves as vital for the acquisition of hypercompetitive advantages, and that the persuasion that originates from privileged information benefits the end consumer.
In view of the need to increase genetic variability to obtain materials with a significant capacity to drought tolerance, this study conducted a cycle of a reciprocal recurrent selection of full-sib ...families of maize. To this end, 64 full-sib families of maize were evaluated in two environments according to their morpho-agronomic data in a randomized block design with two replicates. It were analyzed of Male flowering (MF); Female flowering (FF); Flowering interval (IF); days for flowering (DF); Plant height (PH); Ear height (EH); number of plants at the Stand (NPS); Number of broken plant (NBrP); Number of bedded plants (NBeP); Strawing (St); Ear length (EL); Ear diameter (ED); Ear number (EN); Prolificacy (Pr); Number of diseased ears (NDE); Number of ears attacked by pests (NEP); Ear weight (EW); Yield (YIE) and Total Chlorophyll Index (TCI). The analysis of variance was performed by the F test at 5% significance level, and also the evaluation of genetic parameters. Regarding morpho-agronomic data, the analysis of variance and the analysis of genetic parameters showed that there was no interaction genotype x environment with regard to the genetic variability among the families under study. Lastly, the final selection of the superior genotypes was made on the basis of the ranking of the 40 most productive families, from which, combined with the molecular data, the 30 most productive, most drought-tolerant, and most genetically diverse ones were selected to compose the next cycle of recurrent maize selection aiming water-stress tolerance.
RESUMO: Tendo em vista a necessidade de aumentar a variabilidade genética para obter materiais com significativa capacidade de tolerância à seca, este estudo conduziu um ciclo de seleção recorrente recíproca de famílias de irmãos completos de milho. Para tanto, 64 famílias de irmãos completos de milho foram avaliadas em dois ambientes de acordo com seus dados morfoagronômicos em um delineamento de blocos casualizados com duas repetições. Foram analisados o florescimento masculino (MF); florescimento feminino (FF); Intervalo de florescimento (IF); dias para florescimento (DF); Altura da planta (PH); Altura da espiga (EH); número de plantas na parcela (NPS); Número de planta quebrada (NBrP); Número de plantas com acamadas (NBeP); empalhamento (St); Comprimento da espiga (EL); Diâmetro da espiga (DE); Número de espigas (EN); Prolificidade (Pr); Número de espigas doentes (EQM); Número de espigas atacadas por pragas (NEP); Peso de espiga (EW); Rendimento de grãos (YIE) e Índice de clorofila total (TCI). A análise de variância foi realizada pelo teste F com nível de significância de 5% e também pela avaliação dos parâmetros genéticos. Em relação aos dados morfoagronômicos, a análise de variância e a análise dos parâmetros genéticos mostraram que não houve interação genótipo x ambiente no que diz respeito à variabilidade genética entre as famílias em estudo. Por fim, a seleção final dos genótipos superiores foi feita com base no ranking das 40 famílias mais produtivas, das quais, combinadas com os dados moleculares, foram selecionadas as 30 mais produtivas, mais tolerantes à seca e mais geneticamente diversificadas. para compor o próximo ciclo de seleção recorrente de milho visando tolerância ao estresse hídrico.
Carbapenem resistance in Acinetobacter baumannii has reached extremely high levels worldwide and class D OXA-type carbapenemases are the main associated mechanism. The aim of this study was to ...evaluate the phenotypic and molecular profile of clinical carbapenem-resistant A. baumannii (CRAb) isolates from a southern Brazilian border region. A. baumannii species was identified by the presence of the blaOXA-51 gene and the susceptibility profile was determined by broth microdilution. The main carbapenemases were investigated by PCR and the typing of the CRAb isolates was performed by PFGE. During the study period, a total of 36 CRAb were recovered, 71.4% from respiratory tract samples from ICU patients. High level resistance to aminoglycosides and fluroquinolones were found in contrast to polymyxin B, for which all of CRAb were susceptible. The blaOXA-23 gene was present in 34 isolates and was the only one detected other than blaOXA-51. Molecular typing revealed the presence of four clonal strains, two of them endemic throughout the study period. To the best of our knowledge, our study brings the first data about resistance profile in Acinetobacter in the western border of south of Brazil and make aware of endemic clones of CRAb-producing-OXA-23 in this region of state, contributing for the construction of the national epidemiologic scenario of CRAb.
Systemic sclerosis is a relatively rare connective tissue disorder characterized by severe and progressive fibrosis of the skin. Due to the current lack of available information on this subject, the ...aim of the present study was to assess light touch sensations in the hands of patients with systemic sclerosis.
We completed a cross-sectional comparative study. Light touch sensations were evaluated in 30 individuals, including 15 patients with systemic sclerosis who exhibited changes in the dermis of their hands without loss of the distal phalanx and 15 subjects who did not exhibit changes in the upper limbs (control group). The groups were age- and sex-matched. Tactile sensory evaluations were performed using the Semmes-Weinstein monofilament test and the two-point discrimination test.
Statistically significant differences were found between groups in the monofilament test. The study group had lower scores across all points of the hand when compared with the control group. Differences were also found when dominant and non-dominant hands were compared (p<0.05). Statistically significant differences were found between groups for a subset of the assessed points in the two-point discrimination test.
The results of a monofilament test showed that tactile sensation, specifically light touch and deep pressure sensations, is altered in the hands of systemic sclerosis patients.
LOBBY AND INFLUENCE POWER FROM THE PUBLIC AGENTS Mia-de-Vasconcelos, César Ricardo; Cavalcanti-Nóbrega, Kleber; Lacerda-de-Paula, Gabriel
Dimensión Empresarial,
12/2019, Letnik:
17, Številka:
4
Journal Article
Recenzirano
Odprti dostop
Abstract The present research aims at exploring and deepening the knowledge about the lobby and the influence of power from pressure groups along to public agents in favor of the consumer. This ...qualitative work used a semi-structured interview as a data collecting tool. The results were processed and analyzed through the content analysis technique, presented by analysis categories and subcategories. Based on the investigation, it was concluded that the pressure group and lobby activities presented themselves as vital for the acquisition of hypercompetitive advantages and that the persuasion that originates from privileged information benefits the end consumer.
Tannery sludge has great potential for use in agriculture, however it is a potentially toxic waste, and it is necessary to establish the ideal dose for each crop. The objective of this study was to ...evaluate the influence of fertilization with liquid tannery sludge on growth, chlorophyll a fluorescence and gas exchange of Pennisetum purpureum in two production cycles. A 2 × 6 + 1 split plot scheme was used, where the first factor was composed of two productive cycles, the second factor different fertilizations doses: 0, 30, 70, 110, 150, 190 t ha
−1
of liquid tannery sludge and an additional treatment with nitrogen, phosphorus and potassium control. Plant height, number of leaves and tillers, canopy and stem diameter, leaf area, dry matter, chlorophyll index (SPAD), chlorophyll a fluorescence and gas exchange were evaluated. The highest production of total dry matter was observed in the second production cycle. The range between 72-100 t ha
−1
of liquid tannery sludge showed the greatest gains in growth and production of elephant grass. At doses above 150 t ha
−1
of liquid tannery sludge, the photochemical apparatus begins to show possible damage to the photosystem II (PSII) and reductions in gas exchange.
Moberg Picking-Up Test in patients with hand osteoarthritis Silva, Paula Gabriel, OT, MSc; Jones, Anamaria, PT, PhD; Fernandes, Artur da Rocha Correa, MD, PhD ...
Journal of hand therapy,
10/2017, Letnik:
30, Številka:
4
Journal Article
Recenzirano
Abstract Study Design Clinical measurement. Introduction The Moberg Pick-up Test (MPUT) was previously used to evaluate functional performance in patients with hand inflammatory disease. This is the ...first study using the MPUT in hand osteoarthritis (OA). Purpose of the Study Compare the functional performance (MPUT) in hand OA patients and healthy controls. Methods Fifty hand OA patients and 50 controls were assessed using the MPUT, AUSCAN and Cochin questionnaires, grip and pinch strength, pain using a visual analog scale and a Likert scale regarding difficulty to perform MPUT. Results In the MPUT evaluation, the OA group presented a statistically significant difference from the control group. The OA group spent more time executing test. The grip and pinch strength measurements showed higher values for the control group. The OA group reported a greater difficulty than the control group in performing the test. Conclusion The MPUT is a short and easy to apply test, which can be safely used to assess the functional performance of the hand OA. Level of evidence II.
Strawberries are grown worldwide, and the fruit is known for its flavor, pleasant aroma, and the presence of important nutraceutical compounds. Under temperate conditions, the species is octaploid ...and presents a complex inheritance. Exploring polyploidy in varietal crosses is the main alternative to developing genotypes of high-temperature regions; thus, breeding programs must evaluate the interaction based on parameters, such as the heritability, stability, easy propagation, and adaptability to different soil and climate variations. To estimate the stability and temporal adaptability of pre-selected triple hybrids of day-neutral strawberries, thirty-six experimental genotypes, three commercial genotypes (‘Albion,’ ‘Monterey,’ and ‘Dover’), and four single hybrids (‘RVFS07,’ ‘RVFS06,’ ‘RVDA11,’ and ‘RVCA16’) were evaluated in a protected cultivation from August to February under tropical climate conditions (southern hemisphere) using the mixed linear model (MLM). The genotypes RVFS07M-34, RVFS07M-24, RCDA11M-04, RVFS07M-154, RVFS07M-36, RVFS07M-33, RVFS07M-80, RVFS07M-10, RVDA11M-21, RVDA11M-13, and RVFS06AL-132 had the highest values of total fruit mass, adaptability, and stability. The mean predicted genotypic values of the selected genotypes was 138% higher than the mean of the controls. Therefore, these genotypes have the potential to be released as cultivars.
Use of Prochilodus lineatus meat for burger making Alexandra Pretto; Antonio Cleber da Silva Camargo; Cristiano Miguel Stefanello ...
Brazilian Journal of Food Technology,
2020, Letnik:
23
Journal Article
Recenzirano
Odprti dostop
Abstract The study aimed to elaborate burgers with fillet and pulp (fish carcass meat) of grumatã (Prochilodus lineatus), and to evaluate its centesimal composition, microbiological and sensorial ...properties. Three formulations were prepared. One was prepared with the fillet (0% pulp), and the remaining two contained 15% or 30% pulp (instead of fillet), textured soy protein, wheat flour and condiments. The centesimal and microbiological composition was evaluated in pre-ready burgers. For sensory analysis, the burgers were grilled, and untrained judges evaluated the appearance, color, taste, texture, overall acceptance and purchase intent. Dry matter and fat were higher in formulations containing pulp; while crude protein and ash contents were higher in burgers with 30% pulp compared with those produced with fillet only. The coagulase-positive Staphylococcus and total and fecal coliform counts were within normal values established by the Brazilian legislation. Salmonella spp. was detected in the pre-ready (raw) formulations, but no colony was observed in the cooked preparations. Although no difference was found among formulations for sensory attributes, burgers with 30% pulp presented a higher purchase intention index (50%) than the other formulations. The complete use (fillet and pulp) of grumatã meat shows that this species can be utilized to elaborate distinct alimentary formulations.
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