Numerous researches show that data on an ethnic origin can give additional information for the personified approach in treatment of different diseases. The aim of this study was to compare oxidative ...stress and antioxidant defense parameters in patients with Type 1 Diabetes Mellitus (T1DM) and arterial hypertension (AH) of two ethnic groups. Patients with T1DM (Mongoloids (n=72) and Caucasians (n=50)) and with AH (Mongoloids (n=40) and Caucasians (n=102)) were examined. Spectrophotometric and fluorometric methods were used. In Mongoloid-patients with T1DM there has been marked increased conjugated dienes (СDs) for 34% and increased total antioxidant activity parameter (TAA) for 54%; in Caucasian-patients with T1DM were increased thiobarbituric acid reactants for 50% and decreased TAA level for 23% compare to controls. СDs in Mongoloids and Caucasians with AH compare to the control were increased. TAAs level in Mongoloids with AH were higher compare to control for 20% and superoxide dismutase activity in Mongoloids with AH were higher for 22% compare to the Caucasians. Changes in oxidative stress parameters in Mongoloid-patients are characterized by low intensity, which is probably due to the increased activity of the antioxidant defense system.
Abstract Menopause is a risk factor for oxidative stress. The aim of our study is to assess antioxidant system parameters (α-tocopherol, retinol, reduced glutathione, total antioxidant activity) in ...peri- and postmenopausal women. The antioxidant defense activity by estimation of total antioxidant activity, α-tocopherol, retinol, oxidized and reduced glutathione levels was studied in women of reproductive age ( n = 37), in perimenopausal ( n = 41) and postmenopausal women ( n = 41). In our study we used spectrofluorofotometer methods. Statistical analysis was performed by non-parametric tests with p < 0.05 as the level of significance. The results of our study showed the decrease of α-tocopherol and retinol concentrations and the increase of oxidized glutathione level in blood serum both in perimenopausal and postmenopausal women, the total antioxidant activity of blood serum was decreased in postmenopausal women only. The results of our study demonstrate that decrease of antioxidant defense system resources depends on the menopausal phase.
Background and aimsTofalars are the inhabitants of the Irkutsk region in Russia, living in highland zone. They concern to the small population groups of the North (756 persons). Last year's ...replacement of traditional type of food (many proteins of animal origin) by the European one is marked. Tofalars concern to the Mongoloids. The aim is to estimate state of health and indicators of blood serum at teenagers - tofalars and Caucasian.MethodsFifty-four teenagers aged from twelve to eighteen years old were examined in Alygdzher settlement. Thirty-two of which are tofalars (seventeen boys and fifteen girls) and twenty-two Caucasian (twelve and ten girls). There were no teenagers with overweight among examined ones; the smaller linear dimension is typical to tofalars in comparison with Caucasians living on this territory. Spectrophotometric and fluorometric methods for studying the lipid peroxidation and antioxidant status have been used.ResultsEndocrine system diseases and eating disorders, musculoskeletal system diseases were more often marked at tofalars in comparison with Caucasians (p<0.05). It was established that the average level of total cholesterol and cholesterol of low-density lipoproteins at tofalars more low than at Caucasians (p<0.05). The low levels of lipid peroxidation products - ketodiens and conjugated triens are marked at tofalars, it's 1,36 times lower (p<0.001) in comparison with Caucasians and also decrease of antioxidants indicators - superoxide dismutase and retinol (1,26 times lower, p<0.05).ConclusionsThe differences in health status between tofalars and Caucasians have been observed. The probable explanation to this serves as genetic factors and metabolism features at tofalars in comparison with Caucasians living at the same conditions.
Background and aimsThe period of organogenesis in the embryonic development is critical in terms of occurrence of long-term effects of intoxication. There is evidence that the fetus is exposed to the ...same alcohol level as the mother's body. It is known that an imbalance in the system 'lipid peroxidation-antioxidant defense' leads to the development of oxidative stress, which is accompanied by a decrease in the body's resistance to adverse factors of the external and internal environment. The aim of this study was to identify features of the lipid peroxidation-antioxidant defense system (LPO-AOD) in newborns born from mothers who consumed alcohol during pregnancy.MethodsAn umbilical cord blood of 66 newborns in the control group (group 1) and 74 newborns, whose mothers consumed alcohol in beer form, dry wine and champagne for the entire period of pregnancy (53 newborns, whose mothers consumed less than 2 doses or 750 ml (group 2) and 21 newborns whose mothers consumed from 2 to 11 doses (750-3850 ml)) (group 3) was studied. Spectrophotometric and fluorometric methods for studying the LPO-AOD have been used. All pregnant women received informed consent for the study.ResultsLipid peroxidation process in both groups of newborns was characterised by a statistically significant increasing of diene conjugates content (p1-2=0,006; p1-3<0,001) and decreased values of alpha -tocopherol (p1-2=0,006; p1-3=0,031) and superoxide dismutase activity (p1-2<0,0001; p1-3=0,008) comparing to control group. The reduction of reduced glutathione (GSH) noted in group 2 in comparison with control (p1-2=0,0007). Fall GSH concentration in red blood cells in newborns 2nd and 3rd groups, may be associated with the observed significant decrease of glutathione reductase activity (p1-2<0,0001; p1-3<0,0001) and glutathione-S-transferase (p1-2=0,001; p1-3=0,04) comparing to control group. In general, the decrease in GSH concentration and activity of the enzymes involved in its metabolism in the newborn group 2 has a negative effect on the balance in the LPO-AOD system.ConclusionsThus, the results suggest that even a small amount of alcoholic beverages drunk by women during pregnancy can cause different metabolic changes in the newborns body, in particular, leading to an imbalance of redox exchange, expressed dysfunction of LPO-AOD system.
Background and aimsThe period of organogenesis in the embryonic development is critical in terms of occurrence of long-term effects of intoxication. There is evidence that the fetus is exposed to the ...same alcohol level as the mother’s body. It is known that an imbalance in the system ‘lipid peroxidation-antioxidant defense’ leads to the development of oxidative stress, which is accompanied by a decrease in the body’s resistance to adverse factors of the external and internal environment. The aim of this study was to identify features of the lipid peroxidation-antioxidant defense system (LPO-AOD) in newborns born from mothers who consumed alcohol during pregnancy.MethodsAn umbilical cord blood of 66 newborns in the control group (group 1) and 74 newborns, whose mothers consumed alcohol in beer form, dry wine and champagne for the entire period of pregnancy (53 newborns, whose mothers consumed less than 2 doses or 750 ml (group 2) and 21 newborns whose mothers consumed from 2 to 11 doses (750–3850 ml)) (group 3) was studied. Spectrophotometric and fluorometric methods for studying the LPO-AOD have been used. All pregnant women received informed consent for the study.ResultsLipid peroxidation process in both groups of newborns was characterised by a statistically significant increasing of diene conjugates content (p1−2=0,006; p1−3<0,001) and decreased values of α-tocopherol (p1−2=0,006; p1−3=0,031) and superoxide dismutase activity (p1−2<0,0001; p1−3=0,008) comparing to control group. The reduction of reduced glutathione (GSH) noted in group 2 in comparison with control (p1−2=0,0007). Fall GSH concentration in red blood cells in newborns 2nd and 3rd groups, may be associated with the observed significant decrease of glutathione reductase activity (p1−2<0,0001; p1−3<0,0001) and glutathione-S-transferase (p1−2=0,001; p1−3=0,04) comparing to control group. In general, the decrease in GSH concentration and activity of the enzymes involved in its metabolism in the newborn group 2 has a negative effect on the balance in the LPO-AOD system.ConclusionsThus, the results suggest that even a small amount of alcoholic beverages drunk by women during pregnancy can cause different metabolic changes in the newborns body, in particular, leading to an imbalance of redox exchange, expressed dysfunction of LPO-AOD system.
Background and aimsTofalars are the inhabitants of the Irkutsk region in Russia, living in highland zone. They concern to the small population groups of the North (756 persons). Last year’s ...replacement of traditional type of food (many proteins of animal origin) by the European one is marked. Tofalars concern to the Mongoloids. The aim is to estimate state of health and indicators of blood serum at teenagers – tofalars and Caucasian.MethodsFifty-four teenagers aged from twelve to eighteen years old were examined in Alygdzher settlement. Thirty-two of which are tofalars (seventeen boys and fifteen girls) and twenty-two Caucasian (twelve and ten girls). There were no teenagers with overweight among examined ones; the smaller linear dimension is typical to tofalars in comparison with Caucasians living on this territory. Spectrophotometric and fluorometric methods for studying the lipid peroxidation and antioxidant status have been used.ResultsEndocrine system diseases and eating disorders, musculoskeletal system diseases were more often marked at tofalars in comparison with Caucasians (p<0.05). It was established that the average level of total cholesterol and cholesterol of low-density lipoproteins at tofalars more low than at Caucasians (p<0.05). The low levels of lipid peroxidation products – ketodiens and conjugated triens are marked at tofalars, it’s 1,36 times lower (p<0.001) in comparison with Caucasians and also decrease of antioxidants indicators – superoxide dismutase and retinol (1,26 times lower, p<0.05).ConclusionsThe differences in health status between tofalars and Caucasians have been observed. The probable explanation to this serves as genetic factors and metabolism features at tofalars in comparison with Caucasians living at the same conditions.
An evaluation of oxidative stress and antioxidant defense activity parameters in patients with HIV-infection is of great importance, especially for HIV-positive women of reproductive age planning to ...have children. The aim of this study was to identify antioxidant deficiency and frequency of reproductive disorders correlation in women with HIV-infection.
53 women with HIV infection (mean age - 31.13 ± 4.49 years) were included in the investigations. They were divided into four groups depending on vitamins in blood plasma content (36 women - α-tocopherol level less than 12 μmol/L (group 1); 17 women α-tocopherol level in the range 12-24 μmol/L (group 2); 35 women with retinol level less than 1.05 μmol/L (group 3) and 18 women – retinol level in the range 1.05-2.8 μmol/L (group 4). Spectrophotometric and fluorometric methods used.
Our study proved that women with HIV and α-tocopherol deficiency have higher level of the reproductive system disorders (incidence of menstrual disorders (63%), infertility (36.1%), and a high percentage of failure of the luteal phase) than in the group with normal vitamin content. Increased levels of primary lipid peroxidation products - diene conjugates (1.8 ± 0.9 μmol/L in group 1 patients than those in group 2 (1.3 ± 0.7 μmol/L) (p=0.043), with the absence of significant differences in the content of the thiobarbituric acid reactants in this group were registered.
The results obtained showed a close correlation of α-tocopherol deficiency and disorders of the reproductive system in women with HIV-infection. It is a significant reason to recommend including of antioxidant drugs in HIV-women patients of complex pathogenetic therapy to regulate adequately their reproductive function and possibly to preserve reproductive ability in these women.
Individual biological effects of adaptation to extreme environmental conditions and reserve potentialities of an organism are, largely, genetically determined. One of the universal mechanisms of an ...organism’s defense under extreme conditions is the synthesis of unspecific factors - antioxidants. The aim of this work was to study peculiarities of lipid peroxidation and antioxidant defense in Tofalars versus non-indigenous girls.
Tofalars are the inhabitants of the Siberia, living in highland zone. They concern to the small population groups of the North (756 persons). The blood samples were taken from 35 teenages girls (17 Tofalars (indigenous), average age = 13.94±0.44, and 18 Caucasian (non-indigenous) girls, average age = 14.06±0.42). Spectrophotometric and fluorometric methods for studying the lipid peroxidation and antioxidant status have been used.
A comparative content analysis of the primary lipid peroxidation products - diene conjugates - showed higher values of this parameter in the indigenous girls (1.5 times, p=0.0009), as compared to non-indigenous, with no changes in the content of the final TBA-active products (p>0.05). In antioxidant defense system of native girls, there were registered higher values of the total AOA - 1.45 times higher (p=0.0006), tocopherol - 1.29 times higher (p=0.0227 and retinol -1.34 times higher (p=0.0420) as compared to non-indigenous girls.
The differences in lipid peroxidation and antioxidant defense status between Tofalars and Caucasians have been observed. The probable explanation to this serves as genetic factors and metabolism features at Tofalars in comparison with Caucasians living at the same conditions. We can conclude that under the extreme climate of Tofalars girls of the native ethnos, in contrast to non-indigenous, exhibit adaptive reactions of lipid peroxidation.
