Although there is substantial interest in intermittent fasting as a dietary approach in active individuals, information regarding its effects in elite endurance athletes is currently unavailable. The ...present parallel randomized trial investigated the effects of a particular intermittent fasting approach, called time-restricted eating (TRE), during 4 weeks of high-level endurance training.
Sixteen elite under-23 cyclists were randomly assigned either to a TRE group or a control group (ND). The TRE group consumed 100% of its estimated daily energy needs in an 8-h time window (from 10:00 a.m. to 6:00 p.m.) whilst energy intake in the ND group was distributed in 3 meals consumed between 7:00 a.m. and 9:00 p.m. Fat and fat-free mass were estimated by bioelectrical impedance analysis and VO
and basal metabolism by indirect gas analyzer. In addition, blood counts, anabolic hormones (i.e. free testosterone, IGF-1) and inflammatory markers (i.e. IL-6, TNF-α) were assessed.
TRE reduced body weight (- 2%; p = 0.04) and fat mass percentage (- 1.1%; p = 0.01) with no change in fat-free mass. Performance tests showed no significant differences between groups, however the peak power output/body weight ratio (PPO/BW) improved in TRE group due to weight loss (p = 0.02). Free testosterone and IGF-1 decreased significantly (p = 0.01 and p = 0.03 respectively) in TRE group. Leucocyte count decreased in ND group (p = 0.02) whilst the neutrophils-to-lymphocytes ratio (NLR) decreased significantly (p = 0.03) in TRE group.
Our results suggest that a TRE program with an 8-h feeding window elicits weight loss, improves body composition and increases PPO/BW in elite cyclists. TRE could also be beneficial for reducing inflammation and may have a protective effect on some components of the immune system. Overall, TRE could be considered as a component of a periodized nutrition plan in endurance athletes.
This trial was retrospectively registered at clinicaltrials.gov as NCT04320784 on 25 March 2020.
Background A ketogenic diet (KD) is a nutritional approach, usually adopted for weight loss, that restricts daily carbohydrates under 30 g/day. KD showed contradictory results on sport performance, ...whilst no data are available on team sports. We sought to investigate the influence of a KD on different parameters in semi-professional soccer players. Methods Subjects were randomly assigned to a iso-protein (1.8 g/Kg body weight/day) ketogenic diet (KD) or western diet (WD) for 30 days. Body weight and body composition, resting energy expenditure (REE), respiratory exchange ratio (RER), cross sectional area (CSA) and isometric muscle strength of quadriceps, counter movement jump (CMJ) and yoyo intermittent recovery test time were measured. Results There was a significantly higher decrease of body fat (p = 0.0359), visceral adipose tissue (VAT) (p = 0.0018), waist circumference (p = 0.0185) and extra-cellular water (p = 0.0060) in KD compared to WD group. Lean soft tissue, quadriceps muscle area, maximal strength and REE showed no changes in both groups. RER decreased significantly in KD (p = 0.0008). Yo-yo intermittent test improved significantly (p < 0.0001) in both groups without significant differences between groups. CMJ significantly improved (p = 0.0021) only in KD. Conclusions This is the first study investigating the effects of a KD on semi-professional soccer players. In our study KD athletes lost fat mass without any detrimental effects on strength, power and muscle mass. When the goal is a rapid weight reduction in such athletes, the use of a KD should be taken into account. Trial registration registered retrospectively on Clinical Trial registration number NCT04078971. Keywords: Ketogenic diet, Soccer, Body composition, Yo-yo intermittent test, Muscle cross sectional area, Metabolism
To study static and dynamic postural balance control in elite and sub-elite male volleyball players with or without a history of musculoskeletal lower limb injuries.
Cross-sectional study.
...Laboratory.
Data were obtained from 45 male volleyball players, divided into healthy (CON, n = 28) and previously injured (INJ, n = 17) players by means of an injury self-reported questionnaire.
Static postural balance parameters were recorded carrying out a monopodalic test with the dominant leg on a stabilometric platform. Dynamic postural balance was assessed with the modified Star Excursion Balance Test (mSEBT) in its version based on three directions (anterior, postero-lateral and postero-medial).
No statistically significant differences were detected between CON and INJ for all the static postural balance parameters considered. Conversely, the mSEBT results underlined a statistically significant decrease of the performance in the three directions (P < .001) for INJ with respect to CON.
The mSEBT should be employed in male volleyball players with a history of musculoskeletal lower limb injuries to better assess postural balance control alterations. Moreover, the decrement of the dynamic balance performance may indicate that, before returning to competitions, the rehabilitation protocol should be more focused on dynamic balance exercises.
•A prior history of injury did not affect static balance in male volleyball players.•mSEBT discriminated male volleyball players with and without a history of injuries.•Balance assessment should include the modified SEBT.
Dumbbell curl (DC) and barbell curl in its two variants, straight (BC) or undulated bar (EZ) are typical exercises to train the elbow flexors. The aim of the study was to verify if the execution of ...these three variants could induce a selective electromyographic (EMG) activity of the biceps brachii (BB) and brachioradialis (BR).
Twelve participants performed one set of ten repetitions at 65% of their 1-RM for each variant of curl. Pre-gelled electrodes were applied with an inter-electrode distance of 24 mm on BB and BR. An electrical goniometer was synchronously recorded with EMG signals to determine the concentric and eccentric phases of each variant of curl.
We detected higher activation profile of both BB (
< 0.05) and BR (
< 0.01) during the EZ compared to the DC. Higher levels of activation was found during the concentric phase for only the BR performed with an EZ compared to DC (
< 0.001) and performing BC compared to DC (
< 0.05). The eccentric phase showed a higher activation of the BB muscle in EZ compared to DC (
< 0.01) and in BC compared to DC (
< 0.05). The BR muscle showed a higher activation performing EZ compared to DC (
< 0.01).
The EZ variant may be preferred over the DC variant as it enhances BB and BR EMG activity during the whole range of motion and only in the eccentric phase. The small difference between BC and EZ variants of the BB and BR EMG activity makes the choice between these two exercises a matter of subjective comfort.
In basketball a maximum accuracy at every game intensity is required while shooting. The aim of the present study was to investigate the acute effect of three different drill intensity simulation ...protocols on jump shot accuracy in expert and junior basketball players.
Eleven expert players (age 26 ± 6 yrs, weight 86 ± 11 kg, height 192 ± 8 cm) and ten junior players (age 18 ± 1 yrs, weight 75 ± 12 kg, height 184 ± 9 cm) completed three series of twenty jump shots at three different levels of exertion. Counter Movement Jump (CMJ) height was also measured after each series of jump shots. Exertion's intensity was induced manipulating the basketball drills. Heart rate was measured for the whole duration of the tests while the rating of perceived exertion (RPE) was collected at the end of each series of shots.
Heart rate and rating of perceived exertion (RPE) were statistically different in the three conditions for both expert and junior players. CMJ height remained almost unchanged in both groups. Jump shot accuracy decreased with increasing drills intensity both in experts and junior players. Expert players showed higher accuracy than junior players for all the three levels of exertion (83% vs 64%,
< 0.001; 75% vs 57%,
< 0.05; 76% vs 60%,
< 0.01). Moreover, for the most demanding level of exertion, experts showed a higher accuracy in the last ten shots compared to the first ten shots (82% vs 70%,
< 0.05).
Experts coped better with the different exertion's intensities, thus maintaining a higher level of performance. The introduction of technical short bouts of high-intensity sport-specific exercises into skill sessions should be proposed to improve jump shot accuracy during matches.
Different attentional foci may modify muscle activation during exercises. Our aim was to determine if it is possible to selectively activate the pectoralis major or triceps brachii muscles according ...to specific verbal instructions provided during the bench press exercise. 13 resistance-trained males (25.6±5.4 yrs, 182.7±9.1 cm, 86.4±9.7 kg) underwent an electromyographic signals acquisition of the sternocostal head, clavicular head of the pectoralis major, the anterior deltoid, and the long head of the triceps brachii (LT) during bench press exercise. Participants performed one non-instructed set (NIS) of 4 repetitions at 50% 1-repetition maximum (1-RM) and one NIS of 4 repetitions at 80% 1-RM. Four additional sets of 4 repetitions at 50% and 80% 1-RM were randomly performed with verbal instructions to isolate the chest muscles (chest instructed set, CIS) or to isolate the triceps muscles (triceps instructed set, TIS). Participants showed significantly higher LT activation during TIS compared to non-instructed set both at 50% (p=0.0199) and 80% 1-RM (p=0.0061) respectively. TIS elicited a significant (p=0.0250) higher activation of LT compared to CIS. Our results suggest that verbal instructions seem to be effective for increasing activity of the triceps brachii but not the pectoralis major during the bench press.
Kinesiologic elastic tape is widely used for both clinical and sport applications although its efficacy in enhancing agonistic performance is still controversial. Aim of the study was to verify in a ...group of healthy basketball players whether a neuromuscular taping application (NMT) on ankle and knee joints could affect the kinematic and the kinetic parameters of the jump, either by enhancing or inhibiting the functional performance. Fourteen healthy male basketball players without any ongoing pathologies at upper limbs, lower limbs and trunk volunteered in the study. They randomly performed 2 sets of 5 counter movement jumps (CMJ) with and without application of Kinesiologic tape. The best 3 jumps of each set were considered for the analysis. The Kinematics parameters analyzed were: knees maximal flexion and ankles maximal dorsiflexion during the push off phase, jump height and take off velocity. Vertical ground reaction force and maximal power expressed in the push off phase of the jump were also investigated. The NMT application in both knees and ankles showed no statistically significant differences in the kinematic and kinetic parameters and did not interfere with the CMJ performance. Bilateral NMT application in the group of healthy male basketball players did not change kinematics and kinetics jump parameters, thus suggesting that its routine use should have no negative effect on functional performance. Similarly, the combined application of the tape on both knees and ankles did not affect in either way jump performance.
Lactate detection with electrochemical biosensors is a fundamental part of the sensing and biosensing research field. Together with glucose detection, this topic attracted scientist attention since ...the 60s. Lactate is a metabolite involved in many processes among several fields of application, from food degradation, to sport medicine, cell cultures analysis, and clinical monitoring. However, few electrochemical lactate biosensors are currently present in the market.In this work, we analyzed and compared the standard Lactate Dehydrogenase catalysis mechanism with a two-enzymes commercial method that proved to dramatically boost the catalysis efficiency from 0.6% to 72.5%. Both the mechanisms result in the production of reduced Nicotinamide Adenine Dinucleotide (NADH). Therefore, we analyzed its electrochemical detection performance using commercial screen-printed carbon electrodes (SPCE), along with the possible interferences of common mediums and buffers. We found that these sensors can be used for NADH detection down to a 10 μM concentration with a sensitivity of 33.14 nA/μM at a 456 mV overpotential. Moreover, we analytically defined the interferences effects of Dulbecco's Modified Eagle Medium on the sensors by Electrochemical Impedance Spectroscopy measurements and equivalent electrical circuits modeling.Finally, we tested the two-enzyme method in two case studies, i.e., cell cultures lactate monitoring as a valuable tool to evaluate their growth, and lactate quantification in human sweat to monitor physical exercise intensity. We proved the feasibility of the combination of commercial two-enzymes kit and SPCE as a low-cost, accurate, and sensitive lactate biosensor in different applications, regardless of sample interferences. Keywords: Lactic acid, SPCE, Cyclic voltammetry, Sweat, Cell cultures, DMEM
The analysis of the center of pressure (CoP) trajectory, derived from force platforms, is a widely accepted measure to investigate postural balance control. The CoP trajectory could be analyzed as a ...physiological time-series through a general stochastic modeling framework (i.e., Stabilogram Diffusion Analysis (SDA)). Critical point divides short-term from long-term regions and diffusion coefficients reflect the level of stochastic activity of the CoP. Sample Entropy (SampEn) allows quantifying the CoP complexity in terms of regularity. Thus, this study aimed to understand whether SDA and SampEn could discriminate the neuromuscular control mechanisms underpinning static and dynamic postural tasks. Static balance control and its relationship with dynamic balance control were investigated through the CoP velocity (Mean Velocity) and the area of the 95th percentile ellipse (Area95). Balance was assessed in 15 subjects (age: 23.13 ± 0.99 years; M = 9) over a force platform under two conditions: static (ST) and dynamic, both in anterior-posterior (DAP) and medio-lateral (DML) directions. During the DAP and DML, subjects stood on an unstable board positioned over a force platform. Short-term SDA diffusion coefficients and critical points were lower in ST than in DAP and DML (p < 0.05). SampEn values resulted greater in ST than in DAP and DML (p < 0.001). As expected, lower values of Area95 (p < 0.001) and Mean Velocity (p < 0.001) were detected in the easiest condition, the ST, compared to DAP and DML. No significant correlations between static and dynamic balance performances were detected. Moreover, differences in the diffusion coefficients were detected comparing DAP and DML (p < 0.05). In the anterior-posterior direction, the critical point occurred at relatively small intervals in DML compared to DAP (p < 0.001) and ST (p < 0.001). In the medio-lateral direction, the critical point differed only between DAP and DML (p < 0.05). Overall, SDA analysis pointed out a less tightly regulated neuromuscular control system in the dynamic tasks, with closed-loop corrective feedback mechanisms called into play at different time intervals in the three conditions. SampEn results reflected more attention and, thus, less automatic control mechanisms in the dynamic conditions, particularly in the medio-lateral task. The different neuromuscular control mechanisms that emerged in the static and dynamic balance tasks encourage using both static and dynamic tests for a more comprehensive balance performance assessment.