By analyzing Sounding of the Atmosphere using Broadband Emission Radiometry and the Modern‐Era Retrospective analysis for Research Applications Version 2 reanalysis observations from 2003 to 2020, we ...found that the structural variation of the westward quasi‐2‐day wave (Q2DW) is related to the mean zonal wind in the background atmosphere, and the zonal wavenumber 3 (W3) is more affected by the background atmosphere than the zonal wavenumber 4 (W4). Our study focuses on the spatial structure of ∼50–95 km and ∼50°S–50°N. The spatial structure of W3 and W4 in the Northern and Southern Hemispheres is unimodal and bimodal, while the bimodal structure of W3 in the Southern Hemisphere is more obvious in some years. In the unimodal structure, W3 has a higher (altitude) peak in the Southern Hemisphere (∼82 km) than in the Northern Hemisphere (∼70 km), while W4 peaks at ∼70 km in both hemispheres. In the bimodal structure, W3 in the Southern Hemisphere fluctuates mostly at ∼82 km, followed by ∼68 km, while W3 in the Northern Hemisphere fluctuates mostly at ∼70 km, followed by ∼82 km. The spatial structure of W4 in both hemispheres fluctuates mainly at ∼70 km, followed by ∼82 km. In addition, W3 is stronger in the Southern Hemisphere than the Northern Hemisphere, W4 is stronger in the Northern Hemisphere than the Southern Hemisphere, and peak amplitudes of both W3 and W4 are larger in a bimodal structure than unimodal structure. The diagnostic analysis shows that the interannual and interhemispheric structure changes of Q2DWs may be due to the larger mean flow instabilities, background winds, and refractive index in the mesosphere, which make the bimodal structure of W3 and W4 obtain larger energy for propagation and amplification, resulting in higher (altitude) and larger amplitudes. This indicates that the background atmosphere of the mesosphere can affect the spatial structure of Q2DWs.
Key Points
Wavenumber 3 (W3) and wavenumber 4 (W4) have unimodal and bimodal structural differences in the two hemispheres
The peak amplitudes of bimodal W3 and W4 are larger than those of unimodal W3 and W4 in both hemispheres
Variations in the background atmosphere are responsible for the interannual and interhemispheric structural variability
Intertidal surface sediments collected from Zhelin Bay, the largest mariculture base of eastern Guangdong Province of China, were analyzed for total metal concentrations and chemical speciation. ...Average total metal concentrations (mg/kg) were 0.063 (Cd), 35.69 (Pb), 23.07 (Cr), 7.50 (Ni), 7.95 (Cu), 74.95 (Zn), and 751.32 (Mn). Concentrations of Cd, Cu, Zn, and Mn were significantly higher than the corresponding background values of Zhelin Bay. All studied metals were dominated by residual fractions, whereas the second relatively higher average portions of Cd (24.10%) and Mn (15.17%) were strongly associated with the acid-soluble fraction. Overall, the intertidal surface sediments of Zhelin Bay were only slightly polluted based on the pollution load index (PLI), with a 21% probability of toxicity based on the mean effects range–median quotient. The metals Cd and Mn posed medium to high risk levels based on the method of risk assessment code (RAC).
•Heavy metal fractionation and ecological risk in the intertidal surface sediments of Zhelin Bay were investigated.•The predominant fraction was residual.•The intertidal surface sediments of Zhelin Bay are only slightly polluted, with a 21% probability of toxicity.
As the most powerful antigen-presenting cell type, dendritic cells (DCs) can induce potent antigen-specific immune responses in vivo, hence becoming optimal cell population for vaccination purposes. ...DCs can be derived ex vivo in quantity and manipulated extensively to be endowed with adequate immune-stimulating capacity. After pulsing with cancer antigens in various ways, the matured DCs are administrated back into the patient. DCs home to lymphoid organs to present antigens to and activate specific lymphocytes that react to a given cancer. Ex vivo pulsed DC vaccines have been vigorously investigated for decades, registering encouraging results in relevant immunotherapeutic clinical trials, while facing some solid challenges. With more details in DC biology understood, new theory proposed, and novel technology introduced (featuring recently emerged mRNA vaccine technology), it is becoming increasingly likely that ex vivo pulsed DC vaccine will fulfill its potential in cancer immunotherapy.
Background
The ecotoxicological risk posed by metals and their mixtures in sediments depends on their bioavailability. Many methods for evaluating the bioavailability of metals in sediments/soils are ...time-consuming and expensive, and frequently result in equivocal outcomes. The diffusive gradients in thin films (DGT) technique is a good measure of bioavailability for metals that can avoid the above drawbacks. Therefore, more effective approaches to this method should be developed that focus on metal bioavailability. No studies have been conducted using DGT to assess metal mixtures to aquatic biota in sediments. This study is therefore the first attempt to assess sediment toxicity of metals and their mixtures to aquatic biota based on the DGT technique. The intertidal zone of the Pearl River Estuary is selected as a case study.
Results
The bioavailable (DGT-labile) concentrations of metals range as follows (μg/L): Cd, 0.34–3.62; Pb, 1.35–1.92; Ni, 0.67–92.83; Cu, 0.74–10.30; Zn, 28.60–296.94; Co, 0.03–58.85; Fe, 7.23–4539.36; and Mn, 19.40–6626.83. The risk quotient (RQ), which is the ratio between the measured metal concentrations in the environment (MEC) and the predicted no-effect concentration (PNEC), is conducted to evaluate the single metal risk. The RQ based on summing up the MEC/PNEC ratios (RQ
MEC/PNEC
) and the RQ based on sum of toxic units (RQ
STU
) are used to assess risk of metal mixture. TheRQ values of Cd, Pb, Ni, Cu, Zn, Fe, and Mn significantly exceed 1, indicating that the adverse effects of the metals are not negligible. Regarding the toxicity of metal mixtures, the values of RQ
MEC/PNEC
and RQ
STU
are both between 62.45 and 743.48, revealing that the possible risk has already occurred in the study area.
Conclusions
The two methods of RQ
MEC/PNEC
and RQ
STU
based on DGT-labile metal concentrations are effective and suitable to estimate the toxicity of metal mixtures in sediments.
Testicular endothelial cells have been found to play an important role in spermatogenesis and fertility, but their mechanism is obscure. Exosomes released by various cells are recognized as cell-cell ...communication mediators during the initiation and progression of many diseases. Therefore, the current study aimed to investigate the protein and miRNA components of human testicular endothelial cell-derived exosomes (HTEC-Exos) and to explore their potential effects on spermatogenesis. In this study, HTEC-Exos were first isolated by the ultracentrifugation method, and then identified by nanoparticle tracking analysis, transmission electron microscopy (TEM), and western blotting. The characteristics of HTEC-Exos were examined by liquid chromatography-mass spectrometry and microRNA (miRNA) chip analysis. Bioinformatics analysis was performed to explore the potential role of the exosomal content on spermatogenesis. A total of 945 proteins were identified, 11 of which were closely related to spermatogenesis. A total of 2578 miRNAs were identified. Among them, 30 miRNAs demonstrated potential associations with male reproductive disorders, such as azoospermia, and spermatogenesis disorders. In particular, 11 out of these 30 miRNAs have been proven to be involved in spermatogenesis based on available evidence. This study provides a global view of the proteins and miRNAs from HTEC-Exos, suggesting that HTEC-Exos may function as potential effectors during the process of spermatogenesis.
A new, electrophilic difluoromethylthiolating reagent N-difluoromethylthiophthalimide 3 was developed. Reagent 3 can be readily synthesized in four steps from easily available starting materials ...phthalimide and TMSCF2H. N-difluoromethylthiophthalimide 3 is a powerful electrophilic difluoromethylthiolating reagent that allows the difluoromethylthiolation of a wide range of nucleophiles including aryl/vinyl boronic acids, alkynes, amines, thiols, β-ketoesters, and oxindoles and electron-rich heteroarenes such as indole, pyrrole, 1H-pyrrolo2,3-bpyridine, imidazo1,2-apyridine, aminothiazole, isoxazole, and pyrazole under mild conditions.
Fabry Perot interferometer (FPI) is an essential ground‐based passive optical observation device to detect middle and upper atmospheric information. Three FPIs are located at Kunming (103.8°E, ...25.6°N), Xinglong (117.4°E, 40.2°N), and Mohe (122.3°E, 53.5°N), China. The diurnal and annual variation of night wind at 87, 97, and 250 km are investigated from 2019 to 2021, compared to Horizontal Wind Model 14 (HWM14) to check the prediction accuracy for the local wind field. Our results are as follows: (a) At 87 km, the zonal winds are similar in Kunming and Xinglong, but the meridional winds are generally stronger in Kunming. The zonal and meridional winds in both locations are dominated by semidiurnal variations. (b) At 97 km, the duration of semidiurnal variation of zonal winds and diurnal variation of meridional winds in Kunming is long, which is opposite to that of Xinglong. (c) At 250 km, the wind speed increases with latitude for both zonal and meridional winds, which are both dominated by diurnal variations for all the three sites. Unlike 87 and 97 km, the entire wind field at 250 km is dominated by annual variation, except for a significant semiannual variation at midnight in Mohe. (d) Overall, the HWM14 predictions are stronger than the FPI measurements at peak wind speeds and similar at 87 and 97 km, which surpasses the performance at 250 km. Especially at 250 km, the model results are worse for the zonal winds of Kunming and the meridional winds of Mohe. These results will help to understand the wind field in the middle and upper atmosphere and improve the accuracy of the model.
Key Points
Characteristics of mesospheric and thermospheric winds over the three stations are studied using three FPIs and compared with HWM14
The winds over the three observatories showed latitudinal variation characteristics
Overall, HWM14 predicts stronger winds than FPI measurements
The total concentrations and oral bioaccessibility of heavy metals in surface-exposed lawn soils from 28 urban parks in Guangzhou were investigated, and the health risks posed to humans were ...evaluated. The descending order of total heavy metal concentrations was Fe > Mn > Pb > Zn > Cu > Cr > Ni > Cd, but Cd showed the highest percentage bioaccessibility (75.96%). Principal component analysis showed that Grouped Cd, Pb, Cr, Ni, Cu and Zn, and grouped Cr and Mn could be controlled two different types of human sources. Whereas, Ni and Fe were controlled by both anthropogenic and natural sources. The carcinogenic risk probabilities for Pb and Cr to children and adults were under the acceptable level (<1 × 10−4). Hazard Quotient value for each metal and Hazard Index values for all metals studied indicated no significant risk of non-carcinogenic effects to children and adults in Guangzhou urban park soils.
•Heavy metals in urban park soils of Guangzhou were investigated.•Cd, Pb, Ni, Cu, Zn, and Mn were polluted.•Cd showed the highest percentage bioaccessibility.•No potential non-carcinogenic risk for children and adults.•The carcinogenic risk probability of Pb and Cr were below 1 × 10−4.
A new method consisting of enrichment factor (EF) determination, nonmetric multidimensional scaling (NMS), and the geographic information system (GIS) technique was firstly developed to identify ...anthropogenic heavy metal sources in marine sediments of Hong Kong. Firstly, the EF was determined to differentiate between heavy metals originating from human and natural sources. Subsequently, NMS was applied to identify various source patterns of heavy metals, and the NMS score was calculated and spatially interpolated using GIS technology to evaluate the spatial influences of anthropogenic impacts in different areas. The concentrations of heavy metals in sediments of Hong Kong substantially exceeded their background values, demonstrating anthropogenic pollution. Two different types of human sources could be identified via NMS, one representing the industrial pollution discharges in the period from the 1960s to the 1980s before pollution control was introduced and one representing sewage discharge before the Tolo Harbour Action Plan in the mid-1980s.
Inspired by the epidermal–dermal and outer microstructures of the human fingerprint, a novel flexible sensor device is designed to improve haptic perception and surface texture recognition, which is ...consisted of single‐walled carbon nanotubes, polyethylene, and polydimethylsiloxane with interlocked and outer micropyramid arrays. The sensor shows high pressure sensitivity (−3.26 kPa−1 in the pressure range of 0−300 Pa), and it can detect the shear force changes induced by the dynamic interaction between the outer micropyramid structure on the sensor and the tested material surface, and the minimum dimension of the microstripe that can be discerned is as low as 15 µm × 15 µm (interval × width). To demonstrate the texture discrimination capability, the sensors are tested for accurately discerning various surface textures, such as the textures of different fabrics, Braille characters, the inverted pyramid patterns, which will have great potential in robot skins and haptic perception, etc.
Fingerprint‐inspired flexible tactile sensor shows high pressure sensitivity (−3.26 kPa−1), and the minimum dimension of the microstripe that can be discerned is as low as 15 µm × 15 µm. The tactile sensor can accurately discern various surface textures, such as different fabrics, Braille characters, and the inverted pyramid patterns, which will have great potential in robot skins and haptic perception.
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