Phytophthora capsici is a highly destructive pathogen of crops. Although chemical pesticides are the most widely used strategy to counter phytopathogens, they have been inefficient to combat P. ...capsici and have produced significant environmental and health problems. Therefore, sustainable alternatives to control soilborne pathogens, such as the inhibitory effect of self‐extracellular DNA (eDNA), have been proposed. This inhibition phenomenon has been attributed to the action of self‐eDNA as a damage‐associated molecular pattern (DAMP). Here, we describe the effect of self‐eDNA on P. capsici zoospore germination rate, antioxidant enzymes activity and MAPK gene expression. Also, the effect of P. capsici eDNA on the protection of chilli pepper (Capsicum annuum) plants against P. capsici was investigated. The results highlight that P. capsici can sense 2–500 µg/ml self‐eDNA and induce stress‐related responses like SAK1 gene expression, and superoxide dismutase and catalase activities. Moreover, in vitro zoospore germination rate was suppressed with self‐eDNA concentrations ranging from 50 to 500 µg/ml. Interestingly, drench applications of P. capsici eDNA at 60 and 100 µg/ml on chilli pepper plants did not show any protective effect against the phytopathogen, whereas 2 µg/ml of P. capsici eDNA drench application showed a lower percentage of plants with symptoms and lower disease severity. Moreover, phenols and total flavonoids were increased in chilli pepper plants, therefore inducing plant immunity. This study showed that self‐eDNA acts as a DAMP in P. capsici and provides insight into the use of eDNA for the protection of crops of agronomic interest.
This study showed that self‐eDNA acts as a DAMP in P. capsici inducing stress‐related responses, giving an insight into the use of self‐eDNA in field‐like conditions against the phytopathogen.
Common bean (
L.) is an important crop for food security and for national economics for several countries worldwide. One of the most important factors of risk in common bean production is the fungal ...disease anthracnose caused by
, which, in some cases, causes complete yield losses; this kind of plant disease is usually managed through the application of chemical products such as fungicides that are commonly not accepted by society. This rejection is based on the relationship of pesticides with health damage and environmental contamination. In order to help in solving these drawbacks, the present work proposes the use of electrochemically activated salt solutions (EASSs) as a safer pathogen control agent in crops, due to it having shown an elicitor and biostimulant effect on plants. With this background, this manuscript presents in vitro results of the evaluation of the inhibitory effect for multiple bean pathogens and in vivo results of EASS in the common bean-
pathosystem by evaluation of the infection severity and defense activation, such as secondary metabolite production and antioxidant activity. EASS presence in growth media had a strong inhibitory effect at the beginning of experiments for some of the evaluated fungi. EASSs showed an effect against the development of the disease when applied in specific doses to prevent distress in plants.
Se colectaron de plantas con síntomas de amarillamiento y marchitez en campos comerciales de garbanzo en cinco municipios del sur de Guanajuato, México. De ellas se aislaron e identificaron los ...patógenos presentes en tallo y raíz. De los aislados obtenidos, los que tenían características morfológicas de Sclerotium rolfsii, se confirmó su identidad por secuenciación de los fragmentos ITS obtenidos con los oligonucleótidos: NsiF y Nlb4R, las secuencias obtenidas se compararon con la base de datos del NCBI. De lo dos aislamientos identificados como S. rolfsii por ambas metodologías, se identificó el más virulento, el cual se utilizó para inocular 181 genotipos de garbanzo de los tipos, kabuli y desi. Los resultados indican de acuerdo con el porcentaje de mortandad, que los genotipos de tipo desi ICC3287, ICC 4874, ICC1882, ICC 10259 y WR-315 y de tipo kabuli CUGA 08-3160 presentaron resistencia moderada al patógeno. De estos genotipos resistentes, destaca el ICC 10259 que tiene resistencia genética a cuatro razas de Fusarium oxysporum f. sp. ciceri y a M. phaseolina.
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