In the process industry, ongoing digitization is leading to a considerable information exchange over the entire life cycle of a process plant. Data models are usually created in the initial planning ...phases and these change continuously with further progress including the planning phase itself, plant adaptations after commissioning, and maintenance. The various models from the disciplines involved have a considerable semantic overlap, creating dependencies. Changes in one model inevitably lead to changes in another. This paper presents an approach for networking and synchronization between data models based on these overlaps. The resulting synchronization network provides models which are as free of inconsistencies as possible across different disciplines at any point in time. In addition, first approaches for collaborative conflict resolution are presented. Approaches from model-driven software development and web technologies are adapted to the application domain of the process industry. Typical information models created during the planning phase of a process engineering plant serve as an example.
The concepts for dataspaces range from database management systems to cross-company platforms for data and applications. In this short paper, we present the “Solid Data Space” (SDS), a concept for ...dataspaces that build on top of the (Semantic) Web and Social Linked Data (Solid). Existing Web technologies and Linked Data principles form the foundation for open, decentralized networks for sovereign data exchange between citizens, organizations and companies. Domain-specific dataspace implementations can extend the agreements for communication and collaboration to enable specific functionality. We compare the SDS with principles and components of the emerging International Data Spaces to identify similarities and point out technological differences.
In the present study we report on the identification and characterization of three novel chloroplast-targeted DnaJ-like proteins CDJ3-5, which in addition to their J-domains contain bacterial-type ...ferredoxin domains. In sequence databases we could identify homologues of CDJ3-5 in green algae, moss and higher plants, but not in cyanobacteria. Phylogenetic analyses allowed us to distinguish two clades containing CDJ3/4 and CDJ5 that must have diverged early in the ancestor of the 'green lineage' and have further diversified later on. Molecular and biochemical analysis of CDJ3 and CDJ4 from Chlamydomonas reinhardtii revealed that both proteins are weakly expressed and appear to be localized to the stroma and to thylakoid membranes respectively. The low transcript levels of the CDJ3 and CDJ4 genes declined even further in the initial phase of heat shock, but CDJ3 transcript levels strongly increased after a dark-to-light shift. Accordingly, the Arabidopsis orthologue of CDJ5 was also found to be light-inducible and to be under strong circadian control. CDJ3 and CDJ4 proteins could both be expressed in Escherichia coli and had redox-active Fe-S clusters. In vitro cross-linking studies demonstrated that CDJ3 and CDJ4 interact with chloroplast ATP-bound HSP70B (heat-shock protein 70B), presumably as dimers, and immunoprecipitation studies showed that CDJ3/4 were also in a complex with HSP70B in Chlamydomonas cell extracts. Finally, CDJ3 was found in complexes with apparent molecular masses of approx. 550-2800 kDa, which appeared to contain RNA. We speculate that the CDJ3-5 proteins might represent redox switches that act by recruiting HSP70B for the reorganization of regulatory protein complexes.
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