Smart nanoparticles for cancer therapy Sun, Leming; Liu, Hongmei; Ye, Yanqi ...
Signal transduction and targeted therapy,
11/2023, Letnik:
8, Številka:
1
Journal Article
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Smart nanoparticles, which can respond to biological cues or be guided by them, are emerging as a promising drug delivery platform for precise cancer treatment. The field of oncology, nanotechnology, ...and biomedicine has witnessed rapid progress, leading to innovative developments in smart nanoparticles for safer and more effective cancer therapy. In this review, we will highlight recent advancements in smart nanoparticles, including polymeric nanoparticles, dendrimers, micelles, liposomes, protein nanoparticles, cell membrane nanoparticles, mesoporous silica nanoparticles, gold nanoparticles, iron oxide nanoparticles, quantum dots, carbon nanotubes, black phosphorus, MOF nanoparticles, and others. We will focus on their classification, structures, synthesis, and intelligent features. These smart nanoparticles possess the ability to respond to various external and internal stimuli, such as enzymes, pH, temperature, optics, and magnetism, making them intelligent systems. Additionally, this review will explore the latest studies on tumor targeting by functionalizing the surfaces of smart nanoparticles with tumor-specific ligands like antibodies, peptides, transferrin, and folic acid. We will also summarize different types of drug delivery options, including small molecules, peptides, proteins, nucleic acids, and even living cells, for their potential use in cancer therapy. While the potential of smart nanoparticles is promising, we will also acknowledge the challenges and clinical prospects associated with their use. Finally, we will propose a blueprint that involves the use of artificial intelligence-powered nanoparticles in cancer treatment applications. By harnessing the potential of smart nanoparticles, this review aims to usher in a new era of precise and personalized cancer therapy, providing patients with individualized treatment options.
Antifungal resistance to Candida pathogens increases morbidity and mortality of immunosuppressive patients, an emerging crisis worldwide. Understanding the Candida prevalence and antifungal ...susceptibility pattern is necessary to control and treat candidiasis. We aimed to systematically analyse the susceptibility profiles of Candida species published in the last ten years (December 2011 to December 2021) from mainland China. The studies were collected from PubMed, Google Scholar, and Science Direct search engines. Out of 89 included studies, a total of 44,716 Candida isolates were collected, mainly comprising C. albicans (49.36%), C. tropicalis (21.89%), C. parapsilosis (13.92%), and C. glabrata (11.37%). The lowest susceptibility was detected for azole group; fluconazole susceptibilities against C. parapsilosis, C. albicans, C. glabrata, C. tropicalis, C. guilliermondii, C. pelliculosa, and C. auris were 93.25%, 91.6%, 79.4%, 77.95%, 76%, 50%, and 0% respectively. Amphotericin B and anidulafungin were the most susceptible drugs for all Candida species. Resistance to azole was mainly linked with mutations in ERG11, ERG3, ERG4, MRR1-2, MSH-2, and PDR-1 genes. Mutation in FKS-1 and FKS-2 in C. auris and C. glabrata causing resistance to echinocandins was stated in two studies. Gaps in the studies' characteristics were detected, such as 79.77%, 47.19 %, 26.97%, 7.86%, and 4.49% studies did not mention the mortality rates, age, gender, breakpoint reference guidelines, and fungal identification method, respectively. The current study demonstrates the overall antifungal susceptibility pattern of Candida species, gaps in surveillance studies and risk-reduction strategies that could be supportive in candidiasis therapy and for the researchers in their future studies.
Recent advances in nanotechnology have opened up new avenues for the controlled synthesis of nanoparticles for biomedical and pharmaceutical applications. Chinese herbal medicine is a natural gift to ...humanity, and it has long been used as an antibacterial and anticancer agent. This study will highlight recent developments in the phytonanotechnological synthesis of Chinese herbal medicines to utilize their bioactive components in biomedical and therapeutic applications. Biologically synthesized silver nanoparticles (AgNPs) have emerged as a promising alternative to chemical and physical approaches for various biomedical applications. The comprehensive rationale of combinational or synergistic effects of Chinese herb-based AgNPs synthesis was investigated with superior physicochemical and biological properties, and their biomedical applications, including antimicrobial and anticancer activity and wound healing properties. AgNPs can damage the cell ultrastructure by triggering apoptosis, which includes the formation of reactive oxygen species (ROS), DNA disintegration, protein inactivation, and the regulation of various signaling pathways. However, the anticancer mechanism of Chinese herbal medicine-based AgNPs is more complicated due to the potential toxicity of AgNPs. Further in-depth studies are required to address Chinese herbs' various bioactive components and AgNPs as a synergistic approach to combat antimicrobial resistance, therapeutic efficiency of drug delivery, and control and prevention of newly emerged diseases.
Microbial degradation of dyes is vital to understanding the fate of dyes in the environment. In this study, a fungal strain A-3 and a bacterial strain L-6, which were identified as Aspergillus ...fumigatus and Pseudomonas fluorescens, respectively, had been proven to efficiently degrade crystal violet (CV) dye. The decolorization of CV dye by fungal and bacterial cocultivation was investigated. The results showed that the decolorization rate of cocultures was better than monoculture (P. fluorescens in L-6 (PF), and that of A. fumigatus A-3 (AF)). Furthermore, enzymatic analysis further revealed that Lac, MnP, Lip, and NADH–DCIP reductases were involved in the biodegradation of CV dyes. UV–visible spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and gas chromatography–mass spectrometry (GC–MS) were used to examine the degradation products. GC–MS analysis showed the presence of 4-(dimethylamino) benzophenone, 3-dimethylaminophenol, benzyl alcohol, and benzaldehyde, indicating that CV was degraded into simpler compounds. The phytotoxicity tests revealed that CV degradation products were less toxic than the parent compounds, indicating that the cocultures detoxified CV dyes. As a result, the cocultures are likely to have a wide range of applications in the bioremediation of CV dyes.
Turcz, a perennial herb of the Asteraceae family, is one of the horticultural and medicinal plants used for curing various diseases and is widely distributed in China and other Asian countries. It ...possesses antibacterial, antimetastatic, antiangiogenic, and antioxidant properties along with anticancer potential. However, the intrageneric classification and phylogenetic relationships within
have long been controversial due to the lack of high-resolution molecular markers, and the complete chloroplast (cp) genome sequencing has not been reported with new evolutionary insights. In the present study,
was used as an experimental material, and its genome was sequenced using high-throughput sequencing technology. We assembled the complete cp genome, and a systematic analysis was conducted for
, acquiring the correspondence of its NCBI accession number (OK545755). The results showed that the cp genome of
is a typical tetrad structure with a total length of 152,401 bp, and the genome encodes 133 genes. Analysis of the complete cp genomes of 20 Eupatorieae shows that the number of simple sequence repeats (SSRs) ranged from 19 to 36 while the number of long sequence repeats was 50 in all cases. Eleven highly divergent regions were identified and are potentially useful for the DNA barcoding of Eupatorieae. Phylogenetic analysis among 22 species based on protein-coding genes strongly supported that
is more closely related to
and belongs to the same branch. The genome assembly and analysis of the cp genome of
will facilitate the identification, taxonomy, and utilization of
as well as provide more accurate evidence for the taxonomic identification and localization of Asteraceae plants.
Potato black scurf, caused by Rhizoctonia solani, is a severe soil-borne disease that affects the quality and production of potatoes worldwide. In our study, we used Paenibacillus polymyxa YF and ...Bacillus amyloliquefaciens HT to determine the antagonistic ability of R. solani, with a particular focus on the antagonistic action of P. polymyxa YF to R. solani and its biocontrol effect on potato black scurf. In fermentation, filtrate assay, 50% filtrate of P. polymyxa YF and B. amyloliquefaciens HT inhibited the growth of R. solani by 85.55% and 82.86%, respectively. Microscopic observations showed notable morphological changes with mycelial collapse, atrophy, and deformation following treatment with the antagonistic filtrates. Moreover, cell membrane permeability results showed increased conductivity in bacteria-treated samples compared to the control. P. polymyxa YF exhibited stable colonization on potato plants and secreted various extracellular enzymes (protease, amylase, and cellulase), along with the synthetic substances with growth-promoting effects, such as siderophores and Indole-3-acetic acid (IAA). Whether it is in the excised tissue inoculation or potted experiment, the negative control showed the highest rank of disease symptoms. In the pot experiment, after YF treatment, physiological parameters showed remarkable changes in plant height, root length, stem thickness, and dry and fresh weight. Compared to blank control, the activities of the four resistant enzymes increased significantly in the P. polymyxa YF treatment group. The upregulation in the P. polymyxa YF group was 4.04, 0.54, 0.46, and 3.10 times, respectively. PCR analysis identified genes in both bacterial strains coding for antimicrobial lipopeptides, including fenB, ituC, and srfAA, which are associated with fengycin, iturin, and surfactin synthesis. These findings demonstrated that P. polymyxa YF has a prominent antagonistic effect on R. solani, suggesting its potential as an effective biological control agent for controlling potato black scurf.
Bacterial skin infections represent a major healthcare concern that can delay healing and threaten human health. Silver nanoparticles (AgNPs) have been widely used for antimicrobial purposes; ...however, their high toxicity limits their applications. Therefore, there is an urgent need to develop simple and efficient therapeutic approaches for treating bacterial infections and promoting wound healing. Here, novel tripeptide (Ghk and GhkCu)-modified AgNPs were developed and subsequently evaluated their antibacterial efficacy against four pathogenic bacterial isolates, cytotoxic properties, and therapeutic effects as a topical treatment for infected wounds. Spherical GhkAgNPs and GhkCuAgNPs with average sizes of 45.92 nm and 56.82 nm exhibited potential antibacterial activity, with a MIC concentration of 8 μg/ml against S. aureus and E. coli. Both AgNPs showed superior bactericidal effects against S. aureus, with complete inhibition after 7 days of treatment. Cytotoxicity assays revealed IC50 (half maximal inhibitory concentrations) values ranging from 6.75 to 6.99 µg/ml in L929 cells. GhkAgNPs displayed accelerated cell migration and facilitated healing up to 92% after 12 h. Furthermore, topical applications of GhkAgNPs and GhkCuAgNPs to S. aureus-infected wounds demonstrated enhanced in vivo wound healing efficacy compared to control groups, as evidenced by increased regenerated epidermal thickness, improved collagen deposition, and downregulation of TNF-α expression. Hence concluded that these novel tripeptides Ghk and GhkCu-modified AgNPs exhibited potent antibacterial effects and significantly promoted wound healing properties.
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•Successful synthesis of silver nanoparticles using tripeptides Ghk and GhkCu.•GhkAgNPs and GhkCuAgNPs demonstrated significant antibacterial activity against wound pathogens.•Ghk and GhkCu-modified AgNPs notably promoted cell migration and proliferation in dermal fibroblasts.•GhkAgNPs and GhkCuAgNPs emerged effective agents for wound healing, particularly in addressing pathogenic infections.
Botrytis cinerea is a pathogenic fungus that infests multiple crops, which causes a severe decrease in yield and generates substantial losses in the economy. Palmarosa essential oil (PEO) is a ...primary aromatic compound extracted from palmarosa that is commonly used for scent, medicine, and flavoring foods due to its diverse bioactive properties. In this study, we explored the antifungal activity and the main mechanism of action of PEO against B. cinerea. In addition, the components and control effects of PEO were also studied.
The antifungal assay was tested using the mycelial growth rate method and colony morphology. The constituents of PEO were identified according to gas chromatography/mass spectrometry (GC-MS). The main mechanism of action of PEO was evaluated by measuring representative indicators, which consist of cell contents leakage, excess reactive oxygen species (ROS), and other related indicators. The results indicated that at a concentration of 0.60 ml l-1, PEO exhibits strong antifungal activity against B. cinerea. The PEO mainly included 13 compounds, of which citronellol (44.67%), benzyl benzoate (14.66%), and acetyl cedrene (9.63%) might be the main antifungal ingredients. The study elucidated the main mechanism of action of PEO against B. cinerea, which involved the disruption of cell membrane structure, resulting in altered the cell membrane permeability, leakage of cell contents, and accumulation of excess ROS.
PEO is a satisfactory biological control agent that inhibits B. cinerea in postharvest onions. PEO (0.60 ml l-1) exhibited strong antifungal activity by disrupting the cell membrane structure, altering cell membrane permeability, leading to the cell contents leakage, accumulation of excess ROS and increased level of Malondialdehyde (MDA) compared to the control group.
Soft rot caused by Pectobacterium carotovorum subsp. carotovorum (Pcc) is the most common bacterial disease of postharvest onion. This study aimed to determine the antibacterial activity of oregano ...and clove oils against Pcc and the control of stored soft rot decay in onion. Oregano oil had a minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) of 200 μL L−1, whereas clove oil had a MIC and MBC of 400 and 500 μL L−1, respectively. Treatment with oregano and clove oil could significantly reduce Pcc cell viability and coincided with a gradient change with time. The inhibitory degree on biofilm synthesis was correlated with oregano and clove oil concentrations. Consequently, 1 × and 2 ×MIC treatments could distinctly reduce biofilm adhesion. With high concentrations of oregano and clove oil, abnormal cell morphology along with cell membrane rupture and cellular leakage were observed by scanning electron microscope (SEM), resulting in a substantial increase in extracellular conductivity. Meanwhile, secretion of extracellular cell wall degrading enzymes (CWDEs) of Pcc was declined in varying degrees, with pectinase (Pel) being significantly inhibited (29.82–100 %). In comparison to the control group, RT-qPCR data indicated that oregano and clove oil downregulated the expression of pathogenic genes, resulting in a decrease in bacterial pathogenicity. Under in vivo culturing, the soft rot decay of onion was reduced by 34.07–100 % in a concentration-dependent manner. In short, essential oils (EOs) may mitigate the severity of onion soft rot by inhibiting the Pcc virulence-associated factors such as activity, motility, toxicity, biofilm formation, and enzyme secretion to various degrees. Thus, oregano and clove oil may be used as alternative bio-fungicides to control and treat the severe disease of soft rot caused by Pcc in onions after harvest.
•The antibacterial activity of oregano and clove oil against Pcc was studied.•Changes in morphological of Pcc were observed by SEM.•EOs significantly inhibited the initial cell attachment of biofilms.•The EOs improved the quality of onions under semi-actual storage conditions.
•Angelica root rot can be suppressed by P. polymyxa YF and B. tequilensis SY89.•P. polymyxa YF and B. tequilensis SY89 can function by inhibiting and degrading ENN B.•The GFP-tagged strains of YF and ...SY89 can colonize in Angelica root.•Antagonistic bacteria have significant disease control effect.
Fusarium avenaceum is the predominant pathogen associated with Angelica sinensis root rot, which results in mycotoxin contamination of Angelica, most prominently Enniatin B (ENN B). This study aimed to isolate bacteria capable of combating various phytopathogenic fungi and degrading ENN B to reduce Angelica root rot. Through co-culture with F. avenaceum, the bacterial strains YF and SY89 were isolated from Angelica rhizosphere soil for their antifungal activities. They were identified as Paenibacillus polymyxa and Bacillus tequilensis based on their morphological characteristics and phylogenetic trees constructed using the 16 S rDNA genes sequence. The strains YF and SY89 could produce antimicrobial substance such as surfactin, fengycin, iturin, polyketide synthases and non ribosomal polypeptide synthase detected by Polymerase Chain Reaction (PCR). In addition, strain P. polymyxa YF and B. tequilensis SY89 showed a prominent ability to inhibit synthesis and degrade ENN B in F. avenaceum suspensions and standard samples. The inhibition rate reached 61.93% and 77.64%, respectively, and the degradation rate reached 60.32% and 76.03%, respectively. Angelica pot experiments were conducted to further evaluate the strains YF and SY89 culture ability to promote plant growth and control Angelica root rot to assess its potential agricultural use. The results indicated that strains YF and SY89 could produce IAA and siderophores, which significantly promoted Angelica roots growth. In addition, strains YF and SY89 have the potential to increase the activity of resistant enzymes, thereby inhibiting F. avenaceum infection. The disease index of Angelica roots treated by strain YF and SY89 decreased by 65.38% and 61.54%, respectively. To further elucidate the antagonistic mechanism of YF and SY89, we examined their colonization pattern in the Angelica root using a green fluorescent protein (GFP) marker. The results indicated that YF and SY89 mainly colonized the root surface before migrating into the roots interior part. The present study demonstrated that P. polymyxa YF and B. tequilensis SY89 showed promising prospects for use as a biological control agent against Angelica root rot and ENN B inhibition under field conditions.
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