•Vitamin B6 and Mg were simultaneously determined by CE-C4D.•Vitamin B6 and Mg peaks were well separated within < 10 min.•A simple and reliable method was developed for pharmaceutical/nutraceutical ...samples.•Single-run CE-C4D analysis can replace HPLC and ICP-OES conventional methods.•Acceptable agreement between measured/label claim contents was achieved.
Vitamins and minerals are usually incorporated in pharmaceutical and nutraceutical products, but a simple, rapid, and inexpensive analytical method for their simultaneous determination is still lacking. In this study, we developed a quantification method for pyridoxine (vitamin B6) and magnesium (Mg) by using purpose-made capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) instrument. Main analytical conditions include: fused silica capillary (total length 55 cm, effective length 40 cm, inner diameter 50 μm); background electrolyte consisted of 10 mM L-arginine/acetic acid (pH 5) with 20% acetonitrile; separation voltage + 20 kV; hydrodynamic injection (siphoning at 20 cm in 25 s). Detection limits of vitamin B6 and Mg were 1 and 0.1 mg/L, respectively. Good linearity (R2 > 0.999) was observed for vitamin B6 and Mg calibration curves over concentration ranges of 3–100 and 0.3–200 mg/L, respectively. The method was applied to analyze vitamin B6 and Mg in several pharmaceutical and nutraceutical samples. The analytical results obtained by our method were in good agreement with reference methods (i.e., HPLC for vitamin B6 and ICP-OES for Mg). High-efficient and low-cost CE-C4D method can accordingly serve as a promising tool for concurrent analysis of inorganic and organic species in pharmaceutical and nutraceutical analysis.
This study describes a method for determining fructans in functional foods by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The sample was first ...extracted with hot water. After hydrolyzed by a mixture of sucrase/β-amylase/pullulanase/maltase, the extract solution was treated with sodium borohydride to remove completely free fructose and glucose in the sample. The reaction conditions with sodium borohydride were investigated: 5 mg/mL of sodium borohydride was used, and the reaction time was 20 min. Fructans were then hydrolyzed by endo- and exo-inulinase to completely form fructose and glucose. The content of fructans was calculated based on the amount of fructose and glucose formed after fructans were hydrolyzed. The method was developed and validated on four different food matrices: milk powder, milk liquid, hard capsule, and soft capsule. The results showed that this method has good specificity and linearity (R2 > 0.99). This method also showed good accuracy and precision, with recovery values ranged between 95.0 and 103.6%, while RSD (%) values were 0.45–3.59%. The method also had a low limit of quantification and limit of detection that can quantify food samples with a small amount of fructans. The validated method has been successfully applied to determine the content of fructans in functional foods, and this method provides a valuable tool to evaluate the quality of functional food products.
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The growth of the world population leads to an increase in demand for food consumption. Along with the projected reduction in demand for meat products, a search is underway for a new type of food ...("novel food"), one of the promising options for which are insects. In 2023 the European Commission has registered flour made from house cricket (Acheta domesticus) as a "novel food" for human consumption. Currently, the amino acid composition of both new types of food and the diet that includes them is not regulated. Accordingly, the potential amino acid imbalance in the diet when entomoprotein is included need to be further investigated.
of the study was to characterize the amino acid composition of a simulated diet using protein obtained from house cricket.
. To assess the balance of diets in terms of amino acid composition, a comparative analysis was made of the actual diet containing protein from traditional foods (scenario 1) and the diet with the likely replacement of beef, pork and poultry with a product containing A. domesticus protein (entomoprotein) (scenario 2). The volume of food consumption has been calculated based on the results of the assessment of a sample survey of household budgets. The study included foods with an established value of annual consumption, that was calculated as daily consumption. The content of essential amino acids in food sources of protein, as well as in the domestic cricket protein, was evaluated using the data from relevant sources. Dietary balance was assessed by calculating its digestibility using data on amino acid scoring, the utility of essential amino acids, the excess content of individual essential amino acids, and the comparable excess content of essential amino acids.
. We determined the daily consumption volumes of basic foods, formed consumption scenarios, including with the potential use of a protein product based on entomoprotein. Comparative analysis of the amino acid composition of the diet showed significantly higher content (from 1.4 up to 2.9 times) of amino acids in the diet in scenario 2. The results of calculating the amino acid score and utility coefficient showed that a diet using entomoprotein could provide a better usage of amino acids for protein synthesis compared to the «traditional» diet, however, the digestibility of protein from the traditional diet is higher compared to entomoprotein (96.8 vs 89.1%).
. Despite the fact that the utility of essential amino acids in the scenario of replacing meat products with a product containing A. domesticus entomoprotein is higher, while the digestibility of protein is lower, the differences identified are insignificant.
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