Botrytis cinerea is a pathogenic fungus that infests multiple crops, which causes a severe decrease in yield and generates substantial losses in the economy. Palmarosa essential oil (PEO) is a ...primary aromatic compound extracted from palmarosa that is commonly used for scent, medicine, and flavoring foods due to its diverse bioactive properties. In this study, we explored the antifungal activity and the main mechanism of action of PEO against B. cinerea. In addition, the components and control effects of PEO were also studied.
The antifungal assay was tested using the mycelial growth rate method and colony morphology. The constituents of PEO were identified according to gas chromatography/mass spectrometry (GC-MS). The main mechanism of action of PEO was evaluated by measuring representative indicators, which consist of cell contents leakage, excess reactive oxygen species (ROS), and other related indicators. The results indicated that at a concentration of 0.60 ml l-1, PEO exhibits strong antifungal activity against B. cinerea. The PEO mainly included 13 compounds, of which citronellol (44.67%), benzyl benzoate (14.66%), and acetyl cedrene (9.63%) might be the main antifungal ingredients. The study elucidated the main mechanism of action of PEO against B. cinerea, which involved the disruption of cell membrane structure, resulting in altered the cell membrane permeability, leakage of cell contents, and accumulation of excess ROS.
PEO is a satisfactory biological control agent that inhibits B. cinerea in postharvest onions. PEO (0.60 ml l-1) exhibited strong antifungal activity by disrupting the cell membrane structure, altering cell membrane permeability, leading to the cell contents leakage, accumulation of excess ROS and increased level of Malondialdehyde (MDA) compared to the control group.
•Angelica root rot can be suppressed by P. polymyxa YF and B. tequilensis SY89.•P. polymyxa YF and B. tequilensis SY89 can function by inhibiting and degrading ENN B.•The GFP-tagged strains of YF and ...SY89 can colonize in Angelica root.•Antagonistic bacteria have significant disease control effect.
Fusarium avenaceum is the predominant pathogen associated with Angelica sinensis root rot, which results in mycotoxin contamination of Angelica, most prominently Enniatin B (ENN B). This study aimed to isolate bacteria capable of combating various phytopathogenic fungi and degrading ENN B to reduce Angelica root rot. Through co-culture with F. avenaceum, the bacterial strains YF and SY89 were isolated from Angelica rhizosphere soil for their antifungal activities. They were identified as Paenibacillus polymyxa and Bacillus tequilensis based on their morphological characteristics and phylogenetic trees constructed using the 16 S rDNA genes sequence. The strains YF and SY89 could produce antimicrobial substance such as surfactin, fengycin, iturin, polyketide synthases and non ribosomal polypeptide synthase detected by Polymerase Chain Reaction (PCR). In addition, strain P. polymyxa YF and B. tequilensis SY89 showed a prominent ability to inhibit synthesis and degrade ENN B in F. avenaceum suspensions and standard samples. The inhibition rate reached 61.93% and 77.64%, respectively, and the degradation rate reached 60.32% and 76.03%, respectively. Angelica pot experiments were conducted to further evaluate the strains YF and SY89 culture ability to promote plant growth and control Angelica root rot to assess its potential agricultural use. The results indicated that strains YF and SY89 could produce IAA and siderophores, which significantly promoted Angelica roots growth. In addition, strains YF and SY89 have the potential to increase the activity of resistant enzymes, thereby inhibiting F. avenaceum infection. The disease index of Angelica roots treated by strain YF and SY89 decreased by 65.38% and 61.54%, respectively. To further elucidate the antagonistic mechanism of YF and SY89, we examined their colonization pattern in the Angelica root using a green fluorescent protein (GFP) marker. The results indicated that YF and SY89 mainly colonized the root surface before migrating into the roots interior part. The present study demonstrated that P. polymyxa YF and B. tequilensis SY89 showed promising prospects for use as a biological control agent against Angelica root rot and ENN B inhibition under field conditions.
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•The pathogen F. oxysporum of C. pilosula wilt disease can be suppressed by three strains.•Antagonistic bacteria have significant disease control effect.•Antagonistic bacteria can ...colonize the roots of C. pilosula.•Antagonistic bacteria can promote the growth of C. pilosula.
Codonopsis pilosula wilt disease caused by Fusarium oxysporum poses a serious threat to the commercial quality and yield of C. pilosilua crops in China. In this study, three selected isolates of antagonistic bacteria, Paenibacillus polymyxa YF, Bacillus amyliquefaciens HT, and Bacillus velezensis BQ, were evaluated for their potential ability to control C. pilosula wilt disease. All three strains could colonize the roots of C. pilosula. However, strain BQ had the strongest colonization ability, in that its colonization abundance was stable at 5.2 × 103 CFU/g. Pot experiments showed that each of these three strains may contribute to enhancing C. pilosula’s growth in terms of its plant height, root length, root fresh weight, and root dry weight. All three antagonistic strains were able to effectively suppress the mycelial growth of F. oxysporum by 45 % to 65 %, with strain YF outperforming the other two strains. Similarly, 1 % sterile supernatant filtrate of each strain was capable of reducing by 40 %∼50 % the germination of F. oxysporum conidia. Pot experiments also demonstrated that strain YF was best able to ameliorate symptoms of C. pilosula wilt disease, whereas strain BQ was best able to prevent them. Similar results were obtained from ex vivo experiments, in that the strain YF was superior to the other two strains to ameliorate symptoms, while strain BQ was superior to the other two strains to prevent them. Analysis of resistance enzyme activity revealed that both peroxidase (POD) and phenylalanine ammonia-lyase (PAL) in C. pilosula roots increased significantly, by 4.5–4.7 and 1.7–1.9 times, in response to the three bacterial strain treatments. And resistance enzyme activity of all three bacterial strain treatments were significantly higher than F. oxysporum treatment and the co-treatment group (F. oxysporum + Antagonistic bacteria treatment groups).
White clover (Trifolium repens L.) belongs to the Fabaceae family legume and is cultivated in China for its medicinal properties and ornamental value. White clover is grown around the world for ...forage, turf , green manure and soil conservation purposes (Zhang el al. 2016). In October 2021, an investigation of a 1,000 m2 plant nursery in Lanzhou, China (36°06'N, 103°83'E) found that 80% of White clover plants were infected, and powdery mildew covered 95% of the leaf area. The disease had seriously destroyed the forage quality and reduced the ornamental value. Initially, thin, radial, irregular white colonies appeared on leaves and gradually spread to stems. The white colonies then expanded and thickened to cover upper surface of the leaf, and microscopic hyphae appeared on the bottom of the leaf. In severe cases, the infection resulted in dieback of the leaf. A small area of sporulating fungus was stripped off from the leaf surface with tape and mounted in sterile water for microscopic examination (Mukhtar et al. 2017). Conidiophores were cylindrical, consisting of a foot cell followed by three to four short cells, measuring 75 to 160 × 7 to 10 μm. Conidiophores had straight, cylindric foot cells ranging from 25 to 40 µm long. Singly produced conidia were hyaline and ranged in shape from oblong to cylindrical. Conidia lacked distinct fibrotic bodies and measured 30 to 45 × 15 to 25 μm in length. Long, unbranched germ tubes formed from the ends of the conidia and nipple-shaped appressoria developed on epiphytic mycelia. Based on these morphological characteristics, the pathogen was initially identified morphologically as Erysiphe polygoni (Braun and Cook 2012). To validate the identity, the internal transcribed spacer (ITS) region of the pathogen (SY77) rDNA was amplified by PCR and sequenced using the ITS1/ITS4 primers (White et al. 1990). The resulting sequences were registered to GenBank (GenBank Accession No.OM280998). The ITS sequence of the SY77 was 100% (640/640) identical to E. polygoni (LC009892) on Polygonum aviculare in the United Kingdom and 99% (638/640) identical to E. polygoni (MK685172) on Antigonon leptopus in Taiwan. MEGA 7.0 was used to conduct the neighbor-joining phylogenetic analysis using the ITS sequences from GenBank. The data indicated that the strain SY77 and E. polygoni clustered together on the same branch. Pathogenicity tests were conducted by gently pressing the infected leaves onto five healthy potted White clover plants, while five non-inoculated plants were used as controls (Michael et al. 2021). The plants were maintained in a growth chamber (25 ℃, 14 h light, and 10 h dark period, RH > 80%). After 10 days, the inoculated plants developed powdery mildew symptoms, whereas the control plants remained symptom-free. The fungus on the inoculated plants was re-isolated, re-identified, and confirmed as E. polygoni based on morphological observations and molecular identification. There is no previous report on E. polygoni causing powdery mildew on White clover in China. The powdery mildew caused by E. polygoni on Red clover has been reported in China and Bulgaria, respectively (Yuan el al.1991; Galina el al. 2017). To our knowledge, this is the first report of powdery mildew caused by E. polygoni on White clover in China. References: 1. Zheng, L., et al. 2018. Plant Dis. 102:628. 2. Mukhtar, G., et al. 2017. Plant Dis.101:1, 246. 3. Braun, U., and Cook, R. T. A. 2012. Taxonomic Manual of the Erysiphales (Powdery Mildews), CBS Biodiversity Series No.11. CBS, Utrecht. 4. Michael, R. F., et al. 2021.Plant Dis. First look.( doi.org/ 10.1094/PDIS-09-21-2060-PDN). 5. Yuan, Q. H., el al.1991. Pratacult Sci.05:59 (in Chinese). 6. Galina, N., et al, 2017. BIOTECHNOL Anim Husb.33.127.
