The bed nucleus of the stria terminalis (BNST) is a center of integration for limbic information and valence monitoring. The BNST, sometimes referred to as the extended amygdala, is located in the ...basal forebrain and is a sexually dimorphic structure made up of between 12 and 18 sub-nuclei. These sub-nuclei are rich with distinct neuronal subpopulations of receptors, neurotransmitters, transporters and proteins. The BNST is important in a range of behaviors such as: the stress response, extended duration fear states and social behavior, all crucial determinants of dysfunction in human psychiatric diseases. Most research on stress and psychiatric diseases has focused on the amygdala, which regulates immediate responses to fear. However, the BNST, and not the amygdala, is the center of the psychogenic circuit from the hippocampus to the paraventricular nucleus. This circuit is important in the stimulation of the hypothalamic-pituitary-adrenal axis. Thus, the BNST has been largely overlooked with respect to its possible dysregulation in mood and anxiety disorders, social dysfunction and psychological trauma, all of which have clear gender disparities. In this review, we will look in-depth at the anatomy and projections of the BNST, and provide an overview of the current literature on the relevance of BNST dysregulation in psychiatric diseases.
The bed nucleus of the stria terminalis (BNST) is critical in mediating states of anxiety, and its dysfunction has been linked to stress-related mental disease. Although the anxiety-related role of ...distinct subregions of the anterior BNST was recently reported, little is known about the contribution of the posterior BNST (pBNST) to the behavioral and neuroendocrine responses to stress. Previously, we observed abnormal expression of corticotropin-releasing factor receptor type 2 (CRFR2) to be associated with post-traumatic stress disorder (PTSD)-like symptoms. Here, we found that CRFR2-expressing neurons within the pBNST send dense inhibitory projections to other stress-related brain regions (for example, the locus coeruleus, medial amygdala and paraventricular nucleus), implicating a prominent role of these neurons in orchestrating the neuroendocrine, autonomic and behavioral response to stressful situations. Local CRFR2 activation by urocortin 3 depolarized the cells, increased the neuronal input resistance and increased firing of action potentials, indicating an enhanced excitability. Furthermore, we showed that CRFR2-expressing neurons within the pBNST are critically involved in the modulation of the behavioral and neuroendocrine response to stress. Optogenetic activation of CRFR2 neurons in the pBNST decreased anxiety, attenuated the neuroendocrine stress response, ameliorated stress-induced anxiety and impaired the fear memory for the stressful event. Moreover, activation following trauma exposure reduced the susceptibility for PTSD-like symptoms. Optogenetic inhibition of pBNST CRFR2 neurons yielded opposite effects. These data indicate the relevance of pBNST activity for adaptive stress recovery.
Wildland fire can cause significant damage but is also a natural process that is key to the healthy functioning of many ecosystems worldwide. Primary fuels for a wildland fire are the dead foliage ...and small branches which accumulate as litter on the ground. A cone calorimeter was used to measure the various aspects of these fuels. A single sample of preignition gases from the live leaves of seven plant species were vacuum collected on quality filters and within super-chilled solvent mixtures. GC-TOFMS (1D) and GCxGC-TOFMS (2D) were used to characterize the “white” smoke emissions. The vegetation chemicals were separated into 4 categories: hydrocarbons (CH), oxygenated organics (CHO), unknown peaks (UNK), and organic non-metals (ONM). The multivariate paired Hotelling T2 test determined that the composition of the white smoke as described by the relative number of peaks in the four chemical groups differed significantly between 1D and 2D (Prob > F3,4 = 0.00004). In contrast, the relative peak area percentages in the four chemical groups did not differ between 1D and 2D (Prob > F3,4 = 0.1258). The Molecular Chemical Maps (MCMs) were used to identify chemical trends between the known and unknown chemicals in live oak and longleaf pine. Application of the 2D technique may provide more detailed information necessary to improve the numerical modeling of wildland fire behavior and emissions production.
Adaptations of evidence-based interventions (EBIs) often occur. However, little is known about the reasons for adaptation, the adaptation process, and outcomes of adapted EBIs. To address this gap, ...we conducted a systematic review to answer the following questions: (1) What are the reasons for and common types of adaptations being made to EBIs in community settings as reported in the published literature? (2) What steps are described in making adaptations to EBIs? and (3) What outcomes are assessed in evaluations of adapted EBIs?
We conducted a systematic review of English language publications that described adaptations of public health EBIs. We searched Ovid PubMed, PsycINFO, PsycNET, and CINAHL and citations of included studies for adapted public health EBIs. We abstracted characteristics of the original and adapted populations and settings, reasons for adaptation, types of modifications, use of an adaptation framework, adaptation steps, and evaluation outcomes.
Forty-two distinct EBIs were found focusing on HIV/AIDS, mental health, substance abuse, and chronic illnesses. More than half (62%) reported on adaptations in the USA. Frequent reasons for adaptation included the need for cultural appropriateness (64.3%), focusing on a new target population (59.5%), and implementing in a new setting (57.1%). Common adaptations were content (100%), context (95.2%), cultural modifications (73.8%), and delivery (61.9%). Most study authors conducted a community assessment, prepared new materials, implemented the adapted intervention, evaluated or planned to evaluate the intervention, determined needed changes, trained staff members, and consulted experts/stakeholders. Most studies that reported an evaluation (k = 36) included behavioral outcomes (71.4%), acceptability (66.7%), fidelity (52.4%), and feasibility (52.4%). Fewer measured adoption (47.6%) and changes in practice (21.4%).
These findings advance our understanding of the patterns and effects of modifications of EBIs that are reported in published studies and suggest areas of further research to understand and guide the adaptation process. Furthermore, findings can inform better reporting of adapted EBIs and inform capacity building efforts to assist health professionals in adapting EBIs.
Between 1986 and 1997, nine studies on health effects of oral contraceptives were published by the New England Journal of Medicine or JAMA. All of those studies showed no increased risk of breast ...cancer or protective effects against ovarian and endometrial cancer. Except for one study published in 1986, the major newspapers in the United States essentially ignored these findings, and The New York Times reported on other inconclusive studies that emphasized an oral contraceptive-breast cancer link.
Although concurrent chemoradiation (CRT) and durvalumab consolidation has become a standard treatment for stage III non-small cell lung cancer (NSCLC), clinicopathologic and genomic factors ...associated with its efficacy remain poorly characterized. Here, in a multi-institutional retrospective cohort study of 328 patients treated with CRT and durvalumab, we identify that very high PD-L1 tumor proportion score (TPS) expression ( ≥ 90%) and increased tumor mutational burden (TMB) are independently associated with prolonged disease control. Additionally, we identify the impact of pneumonitis and its timing on disease outcomes among patients who discontinue durvalumab: compared to patients who experienced early-onset pneumonitis ( < 3 months) leading to durvalumab discontinuation, patients with late-onset pneumonitis had a significantly longer PFS (12.7 months vs not reached; HR 0.24 95% CI, 0.10 to 0.58; P = 0.001) and overall survival (37.2 months vs not reached; HR 0.26 95% CI, 0.09 to 0.79; P = 0.017). These findings suggest that opportunities exist to improve outcomes in patients with lower PD-L1 and TMB levels, and those at highest risk for pneumonitis.
► Study of specific Internet use in relation happiness, perceived social support, and introversion. ► Novel measures of six domains of internet use were developed. ► Specific on line activities were ...related to social support, happiness and introversion. ► Persons with high levels of gaming and or mischief may be at greater risk.
The Internet is no longer an advanced technology accessible to a select few. It has become a ubiquitous tool for users ranging from professional programmers to casual surfers and young children. The exponential increase in time online has prompted curiosity and speculation about the interaction between this technology and individual person variables. While general survey data exist regarding broad patterns of Internet use, less is known about the relationship between specific usage and individual personality dimensions, mood variables, or social activity. This study sought to clarify several of these relationships. One hundred eighty-five undergraduate student volunteers completed two detailed measures of Internet use across various domains (for example: work/school, tasks/services, entertainment), as well as measures of happiness, perceived social support, and introversion. Specific types of Internet use, including gaming and entertainment usage, were found to predict perceived social support, introversion and happiness. Use of the Internet for mischief-related activities (for example: downloading without payment, fraud, snooping) was associated with lower levels of happiness and social support. These findings support the utility of and need for specific rather than general Internet research. Directions for future research clarifying the role of the Internet in quality of life and interpersonal relations are suggested.
The above-ground performance of decking treated with two alternative copper-based preservative formulations is being evaluated at a test site near Madison, Wisconsin, USA. Southern pine sapwood ...lumber specimens (38 mm by 140 mm by 910 mm) were pressure treated with 0,93 %, 1,40 % or 2,34 % (oxide basis) actives concentrations of a boron-copper formulation (BC) composed of 7,2 % copper hydroxide and 92,8 % sodium tetraborate decahydrate. Similar specimens were pressure-treated with 0,66 % or 1,32 % actives concentrations of a copper-zinc formulation (CZDP) composed of 18 % copper (CuO basis), 12 % zinc (ZnO basis), 14 % dimethylcocoamine and 56 % propanoic acid. In both cases untreated specimens and specimens treated with a 1% concentration of chromated copper arsenate Type C (CCA-C) were included for comparison. The specimens were installed on racks approximately 760 mm above the ground and periodically evaluated for extent of fungal decay and surface microbial growth. After 18 years in test specimens treated with the lowest solution concentration of BC (0,93 %) suffered substantial degradation and all but three replicates have failed. Obvious decay has not yet been detected in specimens treated to the highest BC concentration (2,34 %), but decay is suspected in one of these specimens. Decking specimens treated with CZDP exhibited no evidence of decay until year 17 when a fruiting body was observed on one specimen treated with a 0,66 % solution concentration. There has been no evidence of decay in specimens treated with 1,32 % CZDP or in either set of specimens treated with 1 % CCA-C. Both BC and CZDP-treated specimens were at least as effective as 1 % CCA-C in minimizing noticeable surface microbial growth. These decking studies confirm that relatively low copper concentrations can provide substantial protection for decking exposed in a moderate climate, and that the CZDP formulation is potentially more effective than the BC formulation. However, caution is warranted in extrapolating these findings to more severe climates and to construction designs that are more likely to trap moisture.
Environmental enrichment (EE) is known to have an anxiolytic effect in several animal models; however, the molecular mechanisms underlying these behavioral changes are not understood. In this study, ...we have shown that the anxiolytic effect of EE is associated with alterations in the corticotropin-releasing factor receptor type 1 (CRFR1) expression levels in the limbic system. We found that the decrease in anxiety-like behavior after housing in enriched conditions was associated with very low levels of CRFR1 mRNA expression in the basolateral amygdala of C57BL/6 mice. We further showed using a lentiviral-based system of RNA interference, that knockdown of CRFR1 mRNA expression in the basolateral amygdala induces a significant decrease in anxiety levels, similar to those achieved by EE nurture. Our data strongly suggest that reduced expression of CRFR1 mRNA levels in the basolateral amygdala mediates the effect of EE on anxiety-like behavior.
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