The European Union (EU) has committed to an ambitious biodiversity recovery plan in its Biodiversity Strategy for 2030 and the Green Deal. These policies aim to halt biodiversity loss and move ...towards sustainable development, focusing on restoring degraded habitats, extending the network of protected areas (PAs), and improving the effectiveness of management, governance, and funding. The achievement of conservation goals must be founded on understanding past successes and failures. Here, we summarise the strengths and weaknesses of past EU biodiversity conservation policies and practices and explore future opportunities and challenges. We focus on four main aspects: i) coordination among and within the EU Member States, ii) integration of biodiversity conservation into socio-economic sectors, iii) adequacy and sufficiency of funds, and iv) governance and stakeholder participation.Whilst past conservation efforts have benefitted from common rules across the EU and funding mechanisms, they have failed at operationalizing coordination within and across the Member States, integrating biodiversity conservation into other sectoral policies, adequately funding and effectively enforcing management, and facilitating stakeholder participation in decision-making. Future biodiversity conservation would benefit from an extended and better-managed network of PAs, additional novel funding opportunities, including the private sector, and enhanced co-governance. However, it will be critical to find sustainable solutions to potential conflicts between conservation goals and other socio-economic objectives and to resolve inconsistencies across sectoral policies.
•The European Union needs more effective conservation as biodiversity declines.•Past success/ failure should guide the implementation of the Biodiversity Strategy.•Better integration of conservation policy into socioeconomic sectors is needed.•More funds, management plans and stakeholder participation are also key.•Adequate planning should guide future efforts to minimise conflicts between sectors.
In the past years, efforts have been made to include connectivity metrics in conservation planning in order to promote and enhance well-connected systems of protected areas. Connectivity is ...particularly important for species that rely on more than one realm during their daily or life cycle (multi-realm species). However, conservation plans for the protection of multi-realm species usually involve a single realm, excluding other realms from the prioritization process. Here, we demonstrate an example of cross-realm conservation planning application for the island of Cyprus by taking into account the terrestrial and marine realms and their interface (i.e. coast). Operating within a data-poor context, we use functional connectivity metrics to identify priority areas for the conservation of six multi-realm species, by setting conservation targets simultaneously for the terrestrial and marine realms. MARXAN decision-support tool was used for the identification of the priority areas.
Four scenarios were developed to evaluate the impacts of including connectivity in the prioritization process and the effectiveness of the existing coastal/marine protected areas in the achievement of the conservation targets set for the species. All scenarios considered land and sea anthropogenic uses as surrogate costs to influence the prioritization process.
Our findings show an increase in the area of the reserve network and, therefore, the cost, when connectivity is included, whilst reducing the total boundary length. Furthermore, the current reserve network fails to achieve conservation targets, particularly for the marine part, which has a substantially smaller protection coverage than the terrestrial part.
We conclude that focus should be given in the expansion of the current coastal/marine reserve network following a cross-realm conservation approach. This approach is not only relevant for the conservation of multi-realm species, but also for islandscapes, in particular, where the interdependence between the hinterland and the coast is larger and therefore the magnitude of the impacts generated in one realm and affects the other.
This paper derives a stochastic discount strategy that encourages the joint consumption of two partial-substitute goods. The seller of the less valuable product offers a limited-availability discount ...to consumers with reference-dependent preferences à la Kőszegi and Rabin (2006). The stochastic discount introduces uncertainty in the consumers’ outcomes and increases their willingness to pay. Due to market competition, such stochastic pricing is effective only when it induces consumers to purchase both products. The other seller, who can only use deterministic pricing, tacitly consents as he has no benefit from engaging in a price war. Thus, a collusive outcome – which harms consumer welfare – is achieved without any explicit coordination between the two sellers.
Epigenetic alterations, including DNA methylation, play an important role in the regulation of gene expression. Several methods exist for evaluating DNA methylation, but bisulfite sequencing remains ...the gold standard by which base-pair resolution of CpG methylation is achieved. The challenge of the method is that the desired outcome (conversion of unmethylated cytosines) positively correlates with the undesired side effects (DNA degradation and inappropriate conversion), thus several commercial kits try to adjust a balance between the two. The aim of this study was to compare the performance of four bisulfite conversion kits Premium Bisulfite kit (Diagenode), EpiTect Bisulfite kit (Qiagen), MethylEdge Bisulfite Conversion System (Promega) and BisulFlash DNA Modification kit (Epigentek) regarding conversion efficiency, DNA degradation and conversion specificity.
Performance was tested by combining fully methylated and fully unmethylated λ-DNA controls in a series of spikes by means of Sanger sequencing (0%, 25%, 50% and 100% methylated spikes) and Next-Generation Sequencing (0%, 3%, 5%, 7%, 10%, 25%, 50% and 100% methylated spikes). We also studied the methylation status of two of our previously published differentially methylated regions (DMRs) at base resolution by using spikes of chorionic villus sample in whole blood.
The kits studied showed different but comparable results regarding DNA degradation, conversion efficiency and conversion specificity. However, the best performance was observed with the MethylEdge Bisulfite Conversion System (Promega) followed by the Premium Bisulfite kit (Diagenode). The DMRs, EP6 and EP10, were confirmed to be hypermethylated in the CVS and hypomethylated in whole blood.
Our findings indicate that the MethylEdge Bisulfite Conversion System (Promega) was shown to have the best performance among the kits. In addition, the methylation level of two of our DMRs, EP6 and EP10, was confirmed. Finally, we showed that bisulfite amplicon sequencing is a suitable approach for methylation analysis of targeted regions.
Under standard assumptions, costly voting models predict that the supporters of the underdog –i.e., of the candidate that is expected to lose– are less likely to abstain than the supporters of the ...expected winner (Palfrey and Rosenthal, 1985; Herrera et al., 2014). While some empirical/experimental studies identify this underdog effect (Levine and Palfrey, 2007), in others bandwagons emerge: the supporters of the expected winner are found to abstain less often than the supporters of the underdog (Agranov et al., 2018). We focus on large elections and follow Kőszegi and Rabin(2006, 2007) by considering that voters experience losses, with respect to their expected equilibrium payoffs, more intensely than gains. When the election is sufficiently close (i.e., when the shares of the supporters of the two alternatives are not too asymmetric), we find that bandwagons emerge in every equilibrium. To our knowledge, this is the first formal study that explains bandwagons in large elections, by incorporating a commonly accepted behavioural model in an otherwise standard context of costly voting.
Context:
Somatotroph adenomas harboring aryl hydrocarbon receptor interacting protein (AIP) mutations respond less well to somatostatin analogs, suggesting that the effects of somatostatin analogs ...may be mediated by AIP.
Objective:
The objective of the investigation was to study the involvement of AIP in the mechanism of effect of somatostatin analogs.
Design:
In the human study, a 16-wk somatostatin analog pretreatment compared with no pretreatment. In the in vitro cell line study, the effect of somatostatin analog treatment or small interfering RNA (siRNA)/plasmid transfection were studied.
Setting:
The study was conducted at a university hospital.
Patients:
Thirty-nine sporadic and 10 familial acromegaly patients participated in the study.
Intervention:
Interventions included preoperative lanreotide treatment and pituitary surgery.
Outcome:
For the human study, GH and IGF-I levels, AIP, and somatostatin receptor staining were measured. For the cell line, AIP and ZAC1 (zinc finger regulator of apoptosis and cell cycle arrest) expression, metabolic activity, and clone formation were measured.
Results:
Lanreotide pretreatment reduced GH and IGF-I levels and tumor volume (all P < 0.0001). AIP immunostaining was stronger in the lanreotide-pretreated group vs. the surgery-only group (P < 0.001). After lanreotide pretreatment, the AIP score correlated to IGF-I changes in females (R = 0.68, P < 0.05). Somatostatin receptor staining was not reduced in samples with AIP mutations. In GH3 cells, 1 nm octreotide increased AIP mRNA and protein (both P < 0.01) and ZAC1 mRNA expression (P < 0.05). Overexpression of wild-type (but not mutant) AIP increased ZAC1 mRNA expression, whereas AIP siRNA knockdown reduced ZAC1 mRNA (both P < 0.05). The siRNA-mediated knockdown of AIP led to an increased metabolic activity and clonogenic ability of GH3 cells compared with cells transfected with a nontargeting control (both P < 0.001).
Conclusion:
These results suggest that AIP may play a role in the mechanism of action of somatostatin analogs via ZAC1 in sporadic somatotroph tumors and may explain their lack of effectiveness in patients with AIP mutations.
We present the first determination of the energy dependence of the B-Dover ¯ and B^{*}-Dover ¯ isospin-0, S-wave scattering amplitudes both below and above the thresholds using lattice QCD, which ...allows us to investigate rigorously whether mixed bottom-charm bover ¯cover ¯ud tetraquarks exist as bound states or resonances. The scattering phase shifts are obtained using Lüscher's method from the energy spectra in two different volumes. To ensure that no relevant energy level is missed, we use large, symmetric 7×7 and 8×8 correlation matrices that include, at both source and sink, B^{(*)}-Dover ¯ scattering operators with the lowest three or four possible back-to-back momenta in addition to local bover ¯cover ¯ud operators. We fit the energy dependence of the extracted scattering phase shifts using effective-range expansions. We observe sharp peaks in the B^{(*)}-Dover ¯ scattering rates close to the thresholds, which are associated with shallow bound states, either genuine or virtual, a few MeV or less below the B^{(*)}-Dover ¯ thresholds. In addition, we find hints for resonances with masses of order 100 MeV above the thresholds and decay widths of order 200 MeV.
In the current study the effect of dilution of chitosan acetate solution and of the use of a reflux-solution method on the morphology, the mechanical and water barrier properties of chitosan based ...nanocomposites is being investigated. Two series of nanocomposite films from two chitosan acetate solutions with 2 w/v% and 1 w/v% in chitosan were prepared, with 3, 5 and 10 wt% Na-montmorillonite (NaMMT) and/or 30 wt% glycerol. Intercalation of NaMMT was more effective in films based on 2 w/v% solutions which presented decreased hydrated crystallinity. Upon NaMMT addition an enhancement was found in stiffness and strength (up to 100%) and a remarkable decrease in the elongation at break (up to 75%) and water vapor permeability (WVP) (up to 65%). This enhancement was less pronounced in 1 w/v% systems. Addition of glycerol had a negative effect on the stiffness, strength and WVP, and a positive effect on the elongation at break and the absorbed water. Compared with the conventional solution cast method, the reflux treatment led to a significant improvement of the tested properties of nanocomposite films.
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