We performed a systematic review and meta-analysis of randomized controlled trials (RCTs) assessing the effect of resistance training in older adults on insulin sensitivity.
Cochrane, Embase, PubMed, ...Web of Science and EBSCO were searched from inception to April 2021. We integrated randomized controlled trials published in English, and participants were non-athletic and aged ≥60 years. The outcome of interest was the change in insulin sensitivity, derived from the homeostatic model of insulin resistance (HOMA-IR) and hemoglobin A1c (HbA1c).
12 RCTs were included in this meta-analysis comparing resistance training (n = 232) with control (n = 209). Resistance exercise significantly reduced HOMA-IR level (d = −0.25, 95% CI, −0.43 to −0.06; P < 0.05) and HbA1c levels of (d = −0.51, 95% CI, −0.84 to −0.18; P < 0.05). Subgroup analysis of HOMA-IR revealed that the variables “population”, “training intensity” and “period” had significant effects on HOMA-IR, with the largest effect sizes for high-intensity (d = −0.43, 95%CI, −0.85 to −0.22, P < 0.05) and long-term (more than 12 weeks) (d = −0.43, 95%CI, −0.85 to −0.22, P < 0.05) training programs in older adults without type 2 diabetes (T2D) (d = −0.23, 95%CI, −0.42 to −0.04, P < 0.05). Subgroup analysis of HbA1c suggested that resistance training programs with moderate intensity (d = −0.51, 95%CI, −0.90 to −0.12, P < 0.05) and short term (less than or equal to 12 weeks) (d = −0.49, 95%CI, −0.84 to −0.14, P < 0.05) have greater effects on HbA1c.
The findings of this meta-analysis suggest that resistance training is effective for inducing improvement in insulin sensitivity for elderly. Subgroup analysis showed that high intensity and long period of resistance exercise improve HOMA-IR in healthy old adults, and that resistance training with moderate intensity and short period improve HbA1c in T2D old people. More studies with high methodological qualities and large sample sizes need to be done to further confirm our conclusion.
This letter presents a 5–6‐GHz dual‐mode power amplifier (PA) realized for further enhanced licensed assisted access application of downlink transmission. The output matching network with multiple ...harmonic traps and the wideband AM‐PM compensation technique are used in the design. To achieve high linear output power with less variation in the wide frequency range, different bias voltages of the first stage PA are set for dual‐mode operation. In the range 5–6 GHz, the PA delivers > 30 dBm saturated output power and reaches 24.4–24.8 dBm linear power at −35 dBc adjacent channel leakage ratio.
Dysregulation of the sterol regulatory element-binding transcription factors sterol regulatory element-binding protein (SREBP) and SREBF activates de novo lipogenesis to high levels in cancer cells, ...a critical event in driving malignant growth. In this study, we identified an important posttranslational mechanism by which SREBP1a is regulated during metabolic reprogramming in cancer cells. Mass spectrometry revealed protein arginine methyltransferase 5 (PRMT5) as a binding partner of SREBP1a that symmetrically dimethylated it on R321, thereby promoting transcriptional activity. Furthermore, PRMT5-induced methylation prevented phosphorylation of SREBP1a on S430 by GSK3β, leading to its disassociation from Fbw7 (FBXW7) and its evasion from degradation through the ubiquitin-proteasome pathway. Consequently, methylation-stabilized SREBP1a increased de novo lipogenesis and accelerated the growth of cancer cells in vivo and in vitro. Clinically, R321 symmetric dimethylation status was associated with malignant progression of human hepatocellular carcinoma, where it served as an independent risk factor of poor prognosis. By showing how PRMT5-induced methylation of SREBP1a triggers hyperactivation of lipid biosynthesis, a key event in tumorigenesis, our findings suggest a new generalized strategy to selectively attack tumor metabolism.
The rapid decrease in sequencing cost has enabled genetic studies to discover rare variants associated with complex diseases and traits. Once this association is identified, the next step is to ...understand the genetic mechanism of rare variants on how the variants influence diseases. Similar to the hypothesis of common variants, rare variants may affect diseases by regulating gene expression, and recently, several studies have identified the effects of rare variants on gene expression using heritability and expression outlier analyses. However, identifying individual genes whose expression is regulated by rare variants has been challenging due to the relatively small sample size of expression quantitative trait loci studies and statistical approaches not optimized to detect the effects of rare variants. In this study, we analyze whole-genome sequencing and RNA-seq data of 681 European individuals collected for the Genotype-Tissue Expression (GTEx) project (v8) to identify individual genes in 49 human tissues whose expression is regulated by rare variants. To improve statistical power, we develop an approach based on a likelihood ratio test that combines effects of multiple rare variants in a nonlinear manner and has higher power than previous approaches. Using GTEx data, we identify many genes regulated by rare variants, and some of them are only regulated by rare variants and not by common variants. We also find that genes regulated by rare variants are enriched for expression outliers and disease-causing genes. These results suggest the regulatory effects of rare variants, which would be important in interpreting associations of rare variants with complex traits.
Late-onset Alzheimer's disease (LOAD) is the most common type of dementia causing irreversible brain damage to the elderly and presents a major public health challenge. Clinical research and ...genome-wide association studies have suggested a potential contribution of the endocytic pathway to AD, with an emphasis on common loci. However, the contribution of rare variants in this pathway to AD has not been thoroughly investigated. In this study, we focused on the effect of rare variants on AD by first applying a rare-variant gene-set burden analysis using genes in the endocytic pathway on over 3,000 individuals with European ancestry from three large whole-genome sequencing (WGS) studies. We identified significant associations of rare-variant burden within the endocytic pathway with AD, which were successfully replicated in independent datasets. We further demonstrated that this endocytic rare-variant enrichment is associated with neurofibrillary tangles (NFTs) and age-related phenotypes, increasing the risk of obtaining severer brain damage, earlier age-at-onset, and earlier age-of-death. Next, by aggregating rare variants within each gene, we sought to identify single endocytic genes associated with AD and NFTs. Careful examination using NFTs revealed one significantly associated gene, ANKRD13D. To identify functional associations, we integrated bulk RNA-Seq data from over 600 brain tissues and found two endocytic expression genes (eGenes), HLA-A and SLC26A7, that displayed significant influences on their gene expressions. Differential expressions between AD patients and controls of these three identified genes were further examined by incorporating scRNA-Seq data from 48 post-mortem brain samples and demonstrated distinct expression patterns across cell types. Taken together, our results demonstrated strong rare-variant effect in the endocytic pathway on AD risk and progression and functional effect of gene expression alteration in both bulk and single-cell resolution, which may bring more insight and serve as valuable resources for future AD genetic studies, clinical research, and therapeutic targeting.
GDF15 is a stress response cytokine and a distant member of the transforming growth factor beta (TGFβ) superfamily, its levels increase in response to cell stress and certain diseases in the serum. ...To exert its effects, GDF15 binds to glial-derived neurotrophic factor (GDNF) receptor alpha-like (GFRAL), which was firstly identified in 2017 and highly expressed in the brain stem. Many studies have demonstrated that elevated serum GDF15 is associated with anorexia and weight loss. Herein, we focus on the biology of GDF15, specifically how this circulating protein regulates appetite and metabolism in influencing energy homeostasis through its actions on hindbrain neurons to shed light on its impact on diseases such as obesity and anorexia/cachexia syndromes. It works as an endocrine factor and transmits metabolic signals leading to weight reduction effects by directly reducing appetite and indirectly affecting food intake through complex mechanisms, which could be a promising target for the treatment of energy-intake disorders.
The vertical migration trend of cyanobacterial cells with gas vesicles in water ecosystems can reflect the changes in the natural environment, such as temperature, nutrients, light conditions, etc. ...The static pressure treatment is one of the most important approaches to study the properties of the cyanobacterial cell and its gas vesicles. In this paper, a polarized light scattering method is used to probe the collapse and regeneration of the cyanobacterial gas vesicles exposed to different static pressures. During the course, both the axenic and wild type strain of cyanobacterial
were first treated with different static pressures and then recovered on the normal light conditions. Combining the observation of transmission electron microscopy and floating-sinking photos, the results showed that the collapse and regeneration of the cyanobacterial gas vesicles exposed to different static pressures can be characterized by the polarization parameters. The turbidity as a traditional indicator of gas vesicles but subjected to the concentration of the sample was also measured and found to be correlated with the polarization parameters. More analysis indicated that the polarization parameters are more sensitive and characteristic. The polarized light scattering method can be used to probe the cyanobacterial gas vesicles exposed to different static pressures, which has the potential to provide an in situ rapid and damage-free monitoring tool for observing the vertical migration of cyanobacterial cells and forecasting cyanobacterial blooms.
This letter presents the architecture and characterization of a Doherty power amplifier (DPA) with a simplified out‐of‐phase power splitter (SOPPS) for fifth‐generation (5G) small‐cell applications. ...The proposed SOPPS consists of two passive components, which is conducive to chip miniaturization. Additionally, a stable DPA architecture is employed to avoid oscillation caused by out‐of‐phase ports imbalance. A DPA based on gallium arsenide (GaAs) heterojunction bipolar transistor (HBT) technology is fabricated, achieving high gain of 32 dB over 2.5 to 2.7 GHz. Using a continuous‐wave signal, the DPA exhibits a saturation output power of 36 dBm, with a peak power‐added efficiency (PAE) of 42.3% and 37% at saturation and 6‐dB power‐back‐off, respectively, across the operating band.
Suspended particles affect the state and vitality of the marine ecosystem. In situ probing and accurately classifying the suspended particles in seawater have an important impact on ecological ...research and environmental monitoring. Individual measurement of the optical polarization parameters scattered by the suspended particles has been proven to be a powerful tool to classify the particulate compositions in seawater. In previous works, the temporal polarized light pulses are sampled and averaged to evaluate the polarization parameters. In this paper, a method based on dense sampling of polarized light pulses is proposed and the experimental setup is built. The experimental results show that the dense sampling method optimizes the classification and increases the average accuracy by at least 16% than the average method. We demonstrate the feasibility of dense sampling method by classifying the multiple types of particles in mixed suspensions and show its excellent generalization ability by multi-classification of the particles. Additional analysis indicates that the dense sampling method basically takes advantage of the high-quality polarization parameters to optimize the classification performance. The above results suggest that the proposed dense sampling method has the potential to probe the suspended particles in seawater in red-tide early warning, as well as sediment and microplastics monitoring.
An easy direct bonding method to join polymers and metals without using complex equipment was developed in this paper. The results showed that microcraters and nanopores were obtained after chemical ...treatment, the interface in cross-section fit well and formed interlocks at the microscale, and the polymer resin had friction interaction at the nanoscale with the metal during de-bonding. The bonding strength and failure work values reached 6.45 MPa and 316 mJ/cm2, respectively. The images of the de-bonded polymer surfaces indicated that the interlocks were established by the polymer which diffused into the pores after the vaporing of the bonding solvent and underwent different degrees of deformation when the metal surface was filled with different scales of complex nanopores network structure, which is a determinant of obtaining bonding strength, while on the other hand, negative factors to the bonding performance were also discussed. The processing procedures were investigated to obtain the effective parameters which could process the Al5052 surface full of nanopores network.
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•An easy direct bonding method to joint dissimilar materials as polymer and metal was practiced•Microscale interlocks were formed between polymer and metal•Nanopores network on treated Al5052 improved the bonding strength and failure work to 6.45 MPa and 316 mJ/cm3•De-bonding mechanisms were analyzed by the pre-bonded/de-bonded surfaces images and macro images, respectively.
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