Ankylosing spondylitis (AS) is a chronic and progressive autoimmune disease affecting the invasion of the spine, sacroiliac joints and peripheral joints. T cells play a vital role in the underlying ...pathogenesis of AS, which mediated autoimmune and inflammatory responses via specific recognition of autoantigen peptides presented by susceptibility HLA. Antigen-specific T cells triggered by HLA/antigen complexes will undergo a massive expansion that forming an uneven T cell repertoire. To enhance our understanding of T-cell-mediated autoimmune in AS, we applied TCR β chains high-throughput sequencing to AS patients for in-depth TCR repertoire analysis. A significantly lower TCR repertoire diversity was observed in peripheral blood of AS patients relative to controls. And severe patients in our AS cohort have a more restricted TCR repertoire than mild patients, suggesting that the TCR repertoire diversity might be associated with the clinical severity of disease. No V, J and VJ pairs with significant biased usage were identified, which indicated that the usage frequency deviation of certain V/J/V-J genes in AS patients is little. This is a pilot study with potentially interesting observation on reduced diversity of T cells repertoire in peripheral blood of AS patients and further studies are needed.
The DEP domain is a globular domain containing approximately 90 amino acids, which was first discovered in 3 proteins: Drosophila disheveled, Caenorhabditis elegans EGL-10, and mammalian Pleckstrin; ...hence the term, DEP. DEPDC1B is categorized as a potential Rho GTPase-activating protein. The function of the DEP domain in signal transduction pathways is not fully understood. The DEPDC1B protein exhibits the characteristic features of a signaling protein, and contains 2 conserved domains (DEP and RhoGAP) that are involved in Rho GTPase signaling. Small GTPases, such as Rac, CDC42, and Rho, regulate a multitude of cell events, including cell motility, growth, differentiation, cytoskeletal reorganization and cell cycle progression.
In this study, we found that it was a guanine nucleotide exchange factor and induced both cell migration in a cultured embryonic fibroblast cell line and cell invasion in cancer cell lines; moreover, it was observed to promote anchorage-independent growth in oral cancer cells. We also demonstrated that DEPDC1B plays a role in regulating Rac1 translocated onto cell membranes, suggesting that DEPDC1B exerts a biological function by regulating Rac1. We examined oral cancer tissue; 6 out of 7 oral cancer tissue test samples overexpressed DEPDC1B proteins, compared with normal adjacent tissue.
DEPDC1B was a guanine nucleotide exchange factor and induced both cell migration in a cultured embryonic fibroblast cell line and cell invasion in cancer cell lines; moreover, it was observed to promote anchorage-independent growth in oral cancer cells. We also demonstrated that DEPDC1B exerts a biological function by regulating Rac1. We found that oral cancer samples overexpressed DEPDC1B proteins, compared with normal adjacent tissue. Suggest that DEPDC1B plays a role in the development of oral cancer. We revealed that proliferation was linked to a novel DEPDC1B-Rac1-ERK1/2 signaling axis in oral cancer cell lines.
Increased emissions from road traffic resulting from the increase in car ownership have put enormous pressure on China’s environmental problems. To solve this problem, the Chinese government has ...attached great importance to the development of a new energy vehicle industry. This paper summarizes the incentive policies of China’s new energy vehicle industry. By sorting through the incentive policy system of the new energy vehicle industry, we find that the Chinese government’s promotion policy for the new energy vehicle industry is a process of gradual transformation from being government-led to being market-led. In this process, with the decrease of the subsidy amount, it is bound to cause a huge impact on the new energy vehicle industry.
(Abstracted from Gynecol Oncol 2023;178:8–13) High-risk human papillomavirus (hrHPV) infection is an essential factor in the development of cervical cancer, making timely treatment of this infection ...important. The number of individuals with persistent hrHPV in the gynecological outpatient setting remains high, and no effective hrHPV drug has been brought to market.
Vessels encapsulating tumor clusters (VETC) represent a recently discovered vascular pattern associated with novel metastasis mechanisms in hepatocellular carcinoma (HCC). However, it seems that no ...one have focused on predicting VETC status in small HCC (sHCC). This study aimed to develop a new nomogram for predicting VETC positivity using preoperative clinical data and image features in sHCC (≤ 3 cm) patients.
To construct a nomogram that combines preoperative clinical parameters and image features to predict patterns of VETC and evaluate the prognosis of sHCC patients.
A total of 309 patients with sHCC, who underwent segmental resection and had their VETC status confirmed, were included in the study. These patients were recruited from three different hospitals: Hospital 1 contributed 177 patients for the training set, Hospital 2 provided 78 patients for the test set, and Hospital 3 provided 54 patients for the validation set. Independent predictors of VETC were identified through univariate and multivariate logistic analyses. These independent predictors were then used to construct a VETC prediction model for sHCC. The model's performance was evaluated using the area under the curve (AUC), calibration curve, and clinical decision curve. Additionally, Kaplan-Meier survival analysis was performed to confirm whether the predicted VETC status by the model is associated with early recurrence, just as it is with the actual VETC status and early recurrence.
Alpha-fetoprotein_lg10, carbohydrate antigen 199, irregular shape, non-smooth margin, and arterial peritumoral enhancement were identified as independent predictors of VETC. The model incorporating these predictors demonstrated strong predictive performance. The AUC was 0.811 for the training set, 0.800 for the test set, and 0.791 for the validation set. The calibration curve indicated that the predicted probability was consistent with the actual VETC status in all three sets. Furthermore, the decision curve analysis demonstrated the clinical benefits of our model for patients with sHCC. Finally, early recurrence was more likely to occur in the VETC-positive group compared to the VETC-negative group, regardless of whether considering the actual or predicted VETC status.
Our novel prediction model demonstrates strong performance in predicting VETC positivity in sHCC (≤ 3 cm) patients, and it holds potential for predicting early recurrence. This model equips clinicians with valuable information to make informed clinical treatment decisions.
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•The elastic constants of Al3Sc exhibit a gentle drop with temperature.•Al3Sc tends to be slightly anisotropic with temperature increasing.•The brittle nature is insensitive to ...temperature.•Strong Al–Sc covalent bonding is only slightly weakened with temperature.
The elastic properties of L12-type Al3Sc at high temperature have been studied using the first-principles calculations combined with the quasi-harmonic approximation. The obtained elastic constants and polycrystalline elastic moduli exhibit a very gentle descent trend with increasing temperature. Elastic anisotropy of Al3Sc is slightly enhanced with increasing temperature, while the brittle nature is insensitive to temperature. The relevant important thermodynamic properties including thermal expansion coefficient and heat capacity are further derived and discussed. The moderate variation of elastic properties and the thermal properties may be attributed to the slightly weakened Al–Sc covalent bonding with temperature increasing. Our results would be valuable for further experimental study of high temperature mechanical properties of the relevant Al-based alloys.
The complexes Cu(bmbp)(HCOO)(H
2
O)(ClO
4
)·DMF (
1
), Co(bmbp)
2
2
(ClO
4
)
4
·DMF·H
2
O (
2
) and Zn(bmbp)
2
2
(ClO
4
)
4
·DMF·H
2
O (
3
), where bmbp is ...4-butyloxy-2,6-bis(1-methyl-2-benzimidazolyl)pyridine, have been synthesized and characterized by physico-chemical methods and single-crystal X-ray diffraction analysis. Complex
1
has a five-coordinate distorted square-pyramidal geometry, while complexes
2
and
3
are six-coordinate with distorted octahedral configurations. The redox properties of complexes
2
and
3
were investigated by cyclic voltammetry. In addition, both the complexes and the free ligand were screened in vitro for their antitumour activities against a human esophageal cancer cell line (Eca109). All three complexes caused inhibition of the growth of Eca109 cells, and complex
1
showed the most effective antitumour activity (IC
50
= 26.09 μM).
Graphical Abstract
Three complexes of copper(II), cobalt(II) and zinc(II) with 4-butyloxy-2,6-bis(1-methyl-2-benzimidazolyl)pyridine were synthesized. The Cu(II) complex has a distorted square-based pyramidal geometry, while the Co(II) and Zn(II) complexes adopt distorted octahedral configurations. The Cu(II) and Co(II) complexes show the irreversible redox processes. All three complexes exhibit inhibition on the growth of Eca109 cells. The IC
50
value of Cu(II) complex is 26.09 μM, which is lower than that of cisplatin (43.99 μM), the Zn(II) complex (46.13 μM) and Co(II) complex (75.46 μM). The Cu(II) complex has potential application in treatment of esophageal cancer.
T cell receptors (TCRs) are a class of T cell surface molecules that recognize the antigen-derived peptides presented by the major histocompatibility complex (MHC) and are able to trigger a series of ...immune responses. TCRs are important members of the adaptive immune system that arose in the jawed fish 500 million years ago. T cell receptor beta variable (TRBV) genes have been widely used to characterize TCR repertoires. Studying the evolution of TRBV may help us to better understand the adaptive immune system. To investigate TRBV evolution and its impacts on the usages of TRBV genes in human populations, we compared the TRBV genes and their homologous sequences among humans, mouse, rhesus and chimpanzee, analyzed the single-nucleotide polymorphisms (SNPs) located at TRBV loci, and sequenced TCR repertoires in the peripheral blood of 97 healthy donors. We found that functional TRBVs are more evolutionarily conserved but possess more SNPs in human populations than do nonfunctional (pseudo) TRBVs. Based on the conservation levels in the four species, we classified the functional TRBVs into 2 groups: old (conserved between mouse and humans) and new (conserved only in primates). The new TRBVs evolve faster and possess more SNPs than the old TRBVs. The variations in TRBV genes frequencies in the peripheral blood of healthy donors are negatively correlated with SNP density. These observations suggest that TRBV usages may be influenced by TCR-MHC co-evolution.
Surface soil (0-20 cm) and peanut kernel samples were collected in four main peanut producing areas of Shandong Province, and the contents of six PAEs chemicals that classified by the U. S. ...Environmental Protection Agency as priority pollutants were determined by gas chromatography (GC). The results indicated that the total concentration of six PAEs (sigma PAEs) ranged from 0.34 to 2.81 mg x kg(-1), and the mean was 1.22 mg x kg(-1). In four different areas, the order of sigmaPAEs concentration in soil was hilly area of middle southern Shandong > western plain of Shandong > Jiaodong Peninsula > northern plain of Shandong. The concentration of DBP in four main peanut producing areas of Shandong Province seriously exceeded the control limit in USA. The content of PAEs ranged from 0.17 to 0.66 mg x kg(-1) in peanut kernels, with the average value 0.34 mg x kg(-1) which was less than the suggested targets in USA and Europe and of low health risk. DEHP and DBP were the main components of PAEs both in soils and pea
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