To decrease the power deficit of a wind farm caused by wake effects, the layout optimization is a feasible way for the wind farm design stage. A suitable optimization algorithm can significantly ...improve the quality and efficiency of the optimization process. For exploring the high-performance algorithms under different layout densities, a comparison is conducted by optimizing the layout of a real offshore wind farm with five algorithms, namely two population-based algorithms and three single-point algorithms. Wake effects are considered by a full-field wake model. A penalty function is proposed for the population-based algorithms to handle the constraint violations. Different iterations and constraints of the layout density are applied in the optimization. The random search has the best optimization results in all the cases and the control of the feasibility check is necessary for this algorithm. More iterations can advance the optimization results. The density constraint greatly affects the computational cost of the random search, which is significantly increased under the strict constraint. Except under the strict constraint, the random search has the best performance of optimization efficiency. A combination of the pattern search and random search is recommended when the strict constraint is applied in the layout optimization.
By optimizing the positions of wind turbines in a wind farm, the power loss caused by wake effects can be reduced maximally. A new methodology of layout optimization is proposed utilizing a ...full-field wake model that integrates the near-field and far-field wake models after modifications, and a random search (RS) algorithm improved with a scale factor for acceleration in high-density layouts. The methodology is applied to a floating wind farm composed of modular platforms, which have a novel configuration and the ability to face toward the wind direction. The applicability and efficiency of the methodology and the improved RS algorithm are validated. The power production of optimized layouts shows a flat crest with an increased number of wind turbines. There is a layout with maximal output power in the wind farm. The real optimal layout should be determined in consideration of both output power and cost. Two sizes of platforms with different number of modules are compared in the application. The wind farm with smaller platforms produces more power. For comparison, a pattern search (PS) algorithm is also implemented in the application. The improved RS algorithm shows outperformance compared with the original RS and the PS algorithm.
Modeling the cyclic shakedown and ratcheting of sand remains a great challenge. An isotropic-kinematic hardening model is presented within the bounding-surface framework. By incorporating new ...features, a combined isotropic-kinematic hardening rule is extended to the deviatoric stress-ratio space. The hardening rule treats cyclic loading as a sequence of monotonic loading events. The kinematic mechanism of this hardening rule assumes that the bounding surface instantaneously translates from the image point to the stress reversal point when the loading path reverses. Then each stress reversal is regarded as an initiation of a new loading event. In each loading event, the isotropic model, which consists of an open wedge-type surface with a circular deviatoric section, may expand or shrink around a generalized homological center to track the variation in the soil fabric. This isotropic mechanism enables the model to capture the gradual evolution of soil stiffness under numerous loading cycles. By introducing a maximum prestress surface, an additional mechanism is deployed to avoid the overshooting problem and to promote the simulation of the post-cyclic behavior. A unified hardening function is defined in the model for all loading events. After calibrating the basic parameters by monotonic tests, only three extra cyclic parameters are required to describe the cyclic behavior of sand. The model behaviors are validated against serval cyclic triaxial tests. The comparison between experimental and predicted results demonstrates the model capabilities in describing the cyclic shakedown and ratcheting responses of sand.
•A combined isotropic-kinematic hardening rule extended to the deviatoric stress-ratio space with new features.•An additional mechanism for avoiding overshooting problem and promoting simulation of the post-cyclic behavior.•Memorizing fabric changes in surface size to capture gradual evolution of soil stiffness.•Only three extra parameters required to predict cyclic response with a unified hardening function.•A novel constitutive model that describes cyclic shakedown and ratcheting of sand.
Astrocyte-mediated inflammation and oxidative stress elicit cerebral ischemia-reperfusion (IR) injury after stroke. Nuclear factor (NF)-κB activates astrocytes and generates pro-inflammatory factors. ...The purpose of the present study is to elucidate the effect of pterostilbene (PTE, a natural stilbene) on astrocytic inflammation and neuronal oxidative injury following cerebral ischemia-reperfusion injury. A middle cerebral artery occlusion-reperfusion (MCAO/R) mouse model and HT22/U251 co-culture model subjected to oxygen-glucose deprivation and re-introduction (OGD/R) were employed, with or without PTE treatment. The data showed that PTE delivery immediately after reperfusion, at 1 h after occlusion, decreased infarct volume, brain edema, and neuronal apoptosis and improved long-term neurological function. PTE decreased oxidation (i.e., production of reactive oxygen species, malondialdehyde) and inflammatory mediators (tumor necrosis factor-α, interleukin-1β, and interleukin-6) and increased anti-oxidative enzyme activities (i.e., of superoxide dismutase, glutathione peroxidase), by inhibiting phosphorylation and nuclear translocation of NF-κB. In conclusion, PTE attenuated astrocyte-mediated inflammation and oxidative injury following IR via NF-κB inhibition. Overall, PTE is a promising neuroprotective agent.
Oxidative stress-related apoptosis and autophagy play crucial roles in the development of osteoarthritis (OA), a progressive cartilage degenerative disease with multifactorial etiologies. Here, we ...determined autophagic flux changes and apoptosis in human OA and tert-Butyl hydroperoxide (TBHP)-treated chondrocytes. In addition, we explored the potential protective effects of trehalose, a novel Mammalian Target of Rapamycin (mTOR)-independent autophagic inducer, in TBHP-treated mouse chondrocytes and a destabilized medial meniscus (DMM) mouse OA model. We found aberrant p62 accumulation and increased apoptosis in human OA cartilage and chondrocytes. Consistently, p62 and cleaved caspase-3 levels increased in mouse chondrocytes under oxidative stress. Furthermore, trehalose restored oxidative stress-induced autophagic flux disruption and targeted autophagy selectively by activating BCL2 interacting protein 3 (BNIP3) and Phosphoglycerate mutase family member 5 (PGAM5). Trehalose could ameliorate oxidative stress-mediated mitochondrial membrane potential collapse, ATP level decrease, dynamin-related protein 1 (drp-1) translocation into the mitochondria, and the upregulation of proteins involved in mitochondria and endoplasmic reticulum (ER) stress-related apoptosis pathway. In addition, trehalose suppressed the cleavage of caspase 3 and poly(ADP-ribose) polymerase (PARP) and prevented DNA damage under oxidative stress. However, the anti-apoptotic effects of trehalose in TBHP-treated chondrocytes were partially abolished by autophagic flux inhibitor chloroquine and BNIP3- siRNA. The protective effect of trehalose was also found in mouse OA model. Taken together, these results indicate that trehalose has anti-apoptotic effects through the suppression of oxidative stress-induced mitochondrial injury and ER stress which is dependent on the promotion of autophagic flux and the induction of selective autophagy. Thus, trehalose is a promising therapeutic agent for OA.
Sepsis is a systemic inflammatory response to infection that causes severe neurological complications. Previous studies have suggested that melatonin is protective during sepsis. Additionally, silent ...information regulator 1 (SIRT1) was reported to be beneficial in sepsis. However, the role of SIRT1 signaling in the protective effect of melatonin against septic encephalopathy remains unclear. This study aimed to investigate the role of SIRT1 in the protective effect of melatonin. EX527, a SIRT1 inhibitor, was used to reveal the role of SIRT1 in melatonin's action. Cecal ligation and puncture or sham operation was performed in male C57BL/6J mice. Melatonin was administrated intraperitoneally (30 mg/kg). The survival rate of mice was recorded for the 7‐day period following the sham or CLP operation. The blood–brain barrier (BBB) integrity, brain water content, levels of inflammatory cytokines (TNF‐α, IL‐1β, and HMGB1), and the level of oxidative stress (superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA)) and apoptosis were assessed. The expression of SIRT1, Ac‐FoxO1, Ac‐p53, Ac‐NF‐κB, Bcl‐2, and Bax was detected by Western blot. The results suggested that melatonin improved survival rate, attenuated brain edema and neuronal apoptosis, and preserved BBB integrity. Melatonin decreased the production of TNF‐α, IL‐1β, and HMGB1. Melatonin increased the activity of SOD and CAT and decreased the MDA production. Additionally, melatonin upregulated the expression of SIRT1 and Bcl‐2 and downregulated the expression of Ac‐FoxO1, Ac‐p53, Ac‐NF‐κB, and Bax. However, the protective effects of melatonin were abolished by EX527. In conclusion, our results demonstrate that melatonin attenuates sepsis‐induced brain injury via SIRT1 signaling activation.
Ischemic stroke is among the leading causes of morbidity and mortality worldwide. Improving the tolerance of neurons to ischemia and reperfusion injury could be a feasible strategy against ischemia. ...Adiponectin (APN) is a major adipokine that regulates glucose and lipid metabolism and plays an important role in the protection of the cerebral nervous system. We aimed to investigate the effects of APN on oxygen and glucose deprivation (OGD)-induced neuronal injury in hippocampal neuronal HT22 cells. APN displayed neuroprotective effects against OGD, evidenced by increased cell viability and decreased lactate dehydrogenase release and apoptotic rate. Additionally, APN also maintained mitochondrial ultrastructure and transmembrane potential, attenuated reactive oxygen species and malondialdehyde, and increased superoxide dismutase and glutathione peroxidase activity. Moreover, APN promoted Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) phosphorylation, enhanced STAT3 nuclear translocation, increased the Bcl-2/Bax ratio, and decreased cleaved caspase-3. The aforementioned APN-induced effects were almost reversed by a JAK2 inhibitor, AG490. APN may attenuate OGD-induced hippocampal HT22 neuronal impairment by protecting cells against mitochondrial oxidative stress and apoptosis, mediated by JAK2/STAT3 signaling.
Purpose
The optimal technique for arthroscopic rotator cuff repair is still controversial. The aim of this study was to compare modified arthroscopic double-pulley suture-bridge (DPSB) technique with ...medial knot tying to those without tying, considering clinical and radiological outcomes.
Methods
This study included 292 patients with large full-thickness rotator cuff tears treated with modified DPSB technique. The patients were divided into 158 cases with medial knot tying (knot-tying group) and 134 without tying (knotless group). At follow-up, clinical outcome was assessed by the Constant score, American Shoulder and Elbow Surgeons (ASES) score, and Shoulder Rating Scale of the University of California at Los Angeles (UCLA) score. The assessment of tendon healing was performed with magnetic resonance imaging (MRI) at a minimum of 12 months postoperatively.
Results
The Constant score, ASES score and UCLA score in the knot-tying and knotless groups all improved significantly from before surgery to 12 months postoperatively (
P
< 0.05, respectively). No significant differences were observed between groups for each phase evaluated (n.s.). Tendon healing was categorised according to Sugaya’s classification. The retearing rate was 27/158 (17.0%) in the knot-tying group and 20/134 (14.9%) in the knotless group, with no statistically significant difference between groups (n.s.). Additionally, the retear was classified using the Cho’s classification. When comparing the retear rates of different types independently, no statistically significant differences were found between groups (n.s.).
Conclusions
The knotless modified DPSB technique showed comparable short-term functional outcomes to those of the knot tying method in large full-thickness rotator cuff tears. Additionally, no significant differences in repair integrity were observed between the two methods. Both techniques can be considered effective treatments for patients with large-sized full-thickness rotator cuff tears.
Level of evidence
III.
Due to abundant wind resources and land saving, offshore wind farms have been vigorously developed worldwide. The wake of wind turbines is an important topic of offshore wind farms, in which the wake ...expansion is a key issue for the wake model and the layout optimization of a wind farm. The large eddy simulation (LES) is utilized to investigate various offshore wind farms under different operating conditions. The numerical results indicate that it is more accurate to calculate the wake growth rate using the streamwise turbulence intensity or the total turbulence intensity in the environment. By fitting the results of the LES, two formulae are proposed to calculate the wake growth rate of the upstream wind turbine. The wake expansion of the downstream wind turbine is analyzed, and the method of calculating the wake growth rate is introduced. The simulation indicates that the wake expansion of the further downstream wind turbines is significantly reduced. The smaller lateral distance of wind turbines in the offshore wind farm has the less wake expansion of the wind turbines. The wake expansion under different inflow wind speeds is also analyzed, while the wake expansion of wind turbines under more complex conditions needs to be further studied.
Abstract Nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) regulates multiple anti-oxidative enzymes and has neuroprotective effects. Pterostilbene (PTE) is a natural anti-oxidant found in ...blueberries. Its non-metabolized form exhibits high distribution in the brain after dietary administration. In this study, we aimed to explore the potential of PTE in protecting murine hippocampal neuronal HT22 cells against glutamate-induced oxidative stress injury and possible underlying mechanisms. PTE was nontoxic and induced the nuclear translocation of Nrf2 when HT22 cell cultures were incubated with different concentrations of PTE. Further, PTE displayed a dose-dependent neuroprotective effect, as indicated by increased cell viability and a reduction in lactate dehydrogenase (LDH) release after glutamate treatment. Nrf2 siRNA treatment inhibited PTE-induced neuroprotective effects. Moreover, the levels of nuclear Nrf2 and downstream heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO1) were elevated after PTE treatment. The PTE-induced elevation of nuclear Nrf2, as well as the increases in HO-1 and NQO1 levels, was abolished by Nrf2 siRNA. PTE treatment reduced the production of reactive oxygen species (ROS) and significantly enhanced the activities of the cellular anti-oxidants glutathione (GSH) and superoxide dismutase (SOD), indicating an attenuation of glutamate-induced oxidative stress. These changes in ROS and GSH and SOD activity were reversed by Nrf2 siRNA. Our results indicate that PTE treatment attenuates glutamate-induced oxidative stress injury in neuronal cells via the Nrf2 signaling pathway.
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