Infection of Taenia pisiformis cysticercus is very frequently found in lagomorphs and causes serious economic losses to rabbit breeding industry. T. pisiformis cysticercus has evolved numerous ...strategies to manipulate their hosts. The release of exosomes is of importance in the interaction between host and parasite. However, the mechanism by which T. pisiformis cysticercus evades the host immune system for long-term survival within the host remains unclear. Using small RNA sequencing and TMT labelling proteomic, we profiled the expression patterns of miRNAs and proteins in rabbit peritoneal macrophages treated with T. pisiformis cysticercus exosomes. Seven differentially expressed (DE)-miRNAs and six DE-proteins were randomly selected to validate the accuracy of the sequencing data by qRT-PCR or western blot. Functions of DE-miRNAs and proteins were analyzed using public data bases. And DE-miRNAs–DE-proteins correlation network were established. CCK-8 assay was used to evaluate the effect of exosomes on macrophages proliferation. Cell cycle of macrophages, isolated from T. pisiformis-infected rabbits, was determined using flow cytometry. A total of 21 miRNAs were significantly differentially expressed, including three worm-derived miRNAs. The expressions of miRNAs and proteins were consistent with the sequencing results. DE-miRNAs targets were related to cell proliferation and apoptosis. Exosomes treatment resulted in a decrease of macrophages proliferation. In vivo, T. pisiformis cysticercus significantly induced S phase cell arrest. Moreover, DE-proteins were related to production of interferon-gamma and interleukin-12, and immunoregulation. Correlation network analysis revealed a negative correlation relationship between DE-miRNAs and DE-proteins. Among them, novel334 and tpi-let-7-5p have potential regulatory effects on IL1β and NFκB2 respectively, which imply that novel334–IL1β/tpi-let-7-5p–NFκB2 axis may be an important way that T. pisiformis cysticercus modulates host immune response through exosomes. Further understanding of these potential regulatory mechanisms will contribute to clarify the mechanism of escape mediated by T. pisiformis exosomes.
•Three worm-derived miRNAs were significantly upregulated in exosomes treated macrophages.•Exosomes treatment resulted in a decrease of macrophages proliferation in a dose-dependent manner.•In macrophages from T. pisiformis cysticercus-infected rabbits, cell cycle was arrested in S phase.•Novel334 and tpi-let-7-5p have potential regulatory effects on IL1β and NFκB2.
To compare the clinical efficacy of single posterior debridement, bone grafting and instrumentation with that of single-stage anterior debridement, bone grafting and posterior instrumentation for ...treatment of adult patients with thoracic and thoracolumbar spinal tuberculosis (TB).
We performed a retrospective analysis of 64 adult patients with thoracic and thoracolumbar spinal TB who underwent surgery between January 2011 and December 2014. Of the 64 patients, 34 patients were treated using a single posterior-only approach (posterior debridement, bone grafting and instrumentation; Group A). Thirty patients were treated with a combined anterior and posterior approach (single-stage anterior debridement, bone grafting and posterior instrumentation; Group B). Clinical manifestations, laboratory and imaging results were subjected to statistical analysis.
The mean (±standard deviation) duration of follow-up was 16.8 ± 1.4 months (range, 10-34). Bony fusion was achieved in all the bone grafts with no loosening or breakage of internal fixation. In both of the groups, the visual analog scale (VAS) pain score, ESR and CRP at 6 weeks after operation and at the most recent follow-up were significantly lower than the preoperative level (p < 0.05). The operation time, intraoperative blood loss and length of hospital stay in group A were significantly less than those in group B (P < 0.05). As of most recent follow-up, no significant between-group difference was observed with respect to the American Spinal Injury Association classification status (p > 0.05). Furthermore, no significant between-group difference was observed with respect to preoperative kyphosis angle, and postoperative angle correction and angle correction rate (P > 0.05). One patient in group A relapsed 20 months after operation, and was successfully treated with debridement using the combined anterior and posterior approach.
Single posterior debridement, bone grafting and instrumentation for treatment of thoracic and thoracolumbar spinal TB can achieve similar curative effect as that with single-stage anterior debridement, bone grafting and posterior instrumentation, and is associated with additional advantages of shorter operation time, less bleeding and shorter length of hospital stay.
Due to the exchangeability of dynamic covalent bonds in the covalent adaptable networks (CANs) at elevated temperature, they possess recyclability while still maintaining many of the superior ...properties of thermosets. The exploration of dynamic covalent chemistry is of great significance to the expansion of CANs library and hence the sustainable development of thermosets. In this work, we discovered that, in absence of catalyst, the direct metathesis of the cyclic acetals proceeds while the acyclic acetals cannot. The metathesis kinetics of the cyclic acetals were fully revealed with model compounds. For the CANs demonstration, a series of cross-linked spirocyclic acetal polymers with excellent reprocessability, high thermal stability, and high refractivity were prepared via thiol–ene click polymerization. We envisage that the uncovering of the catalyst-free metathesis of cyclic acetals will enrich the dynamic chemistry of acetals and greatly promote the development of acetal-based CANs and their potential applications in optical devices.
Polymer nanocomposites (PNCs) are now widely used in various fields. It is modified by adding fillers to improve its various properties during processing and application. In this work, we ...investigated the viscoelasticity of PNCs respectively filled with nanoparticles, nanorods and nanosheets and compared the effects of filler shape and filler size. It is found that the nanorod has the most outstanding ability to increase the modulus of PNCs among the three, followed by the nanosheet. The increase of filler size can increase the modulus of the system and has little effect on Payne effect. The source of modulus enhancement of PNCs respectively filled with nanorods and nanosheets changes with increasing volume fraction from Rouse dynamics of the polymer chains to the binding force of the filler on the polymer chains and eventually to the filler network. Increasing the filler size makes the Rouse dynamics region and chain confinement region narrower and the permeation threshold lower. However, the source of modulus enhancement of PNCs filled with nanoparticles is mainly the Rouse dynamics of polymer chains, which only conforms to the above mechanism at a high interface degree of nanoparticles.
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•The modules enhancement of the nanorods or nanosheets filled PNCs with the volume fraction can be divided into three regions.•The increase of filler size can enhance the modules of PNCs, but has little effect on Payne effect.•The modules enhancement of the nanoparticles filled PNCs mainly comes from Rouse dynamics of polymer chains.
Metformin, an anti-diabetic drug commonly used for type 2 diabetes therapy, is associated with anti-angiogenic effects in conditions beyond diabetes. miR-21 has been reported to be involved in the ...process of angiogenesis. However, the precise regulatory mechanisms by which the metformin-induced endothelial suppression and its effects on miR-21-dependent pathways are still unclear. Bioinformatic analysis and identification of miR-21 and its targets and their effects on metformin-induced antiangiogenic activity were assessed using luciferase assays, quantitative real-time PCR, western blots, scratch assays, CCK-8 assays and tubule formation assays. In this study, miR-21 was strikingly downregulated by metformin in a time- and dose-dependent manner. miR-21 directly targeted the 3'-UTR of PTEN and SMAD7, and negatively regulated their expression. Overexpression of miR-21 abrogated the metformin-mediated inhibition of endothelial cells proliferation, migration, tubule formation and the TGF-β-induced AKT, SMAD- and ERK-dependent phosphorylations, and conversely, down-regulation of miR-21 aggravated metformin's action and revealed significant promotion effects. Our study broadens our understanding of the regulatory mechanism of miR-21 mediating metformin-induced anti-angiogenic effects, providing important implications regarding the design of novel miRNA-based therapeutic strategies against angiogenesis.
To study the nucleation and growth mechanism of Ni/SiC composite coatings, electrodeposition was conducted in the Ni bath containing SiC particles with different sizes. Compared to the Ni coating ...electrodeposited from the bare Ni bath, the Ni/SiC composite coatings exhibited similar grain size but rougher surface morphology due to the incorporation of SiC particles. By means of chronoamperometry (CA) and linear sweep voltammetry (LSV) techniques, it was found that the introduction of SiC particles did not change the nucleation mode of Ni: the Scharifker-Hills three-dimensional instantaneous nucleation mode. However, the nucleation sites on the cathode were reduced due to the inert surface and low conductivity of SiC particles. At a step potential of −0.94 V, Ni presented the maximum nucleation density (N0) of 3.09 × 107 cm−2, which was reduced by one order of magnitude for the Ni/SiC. Furthermore, SiC particles in the bath would extend the diffusion path of Ni2+ ions, resulting in the decrease in diffusion coefficient from 1.04 × 10−7 cm2 s−1 (Ni) to 9.91 × 10−9 cm2 s−1 (Ni/50 nm SiC), 1.83 × 10−8 cm2 s−1 (Ni/500 nm SiC) and 2.29 × 10−8 cm2 s−1 (Ni/5 μm SiC), suggesting the “Blocking effect”. COMSOL simulation further confirmed the “Blocking effect”. The “Blocking effect” would lead to excessive interface gaps around 5 μm SiC particles due to the uneven electrodeposition of Ni. To address the issue, we proposed that adding non-ionic surfactants into the bath to lower the electrochemical reaction rate of Ni2+ ions and accordingly gained composite coatings with fine continuity.
•The effects of ultrasound power and frequency on adhesion strength are investigated quantitatively.•The influence of ultrasound power and frequency on micro electroforming process is ...investigated.•The ultrasonic agitation method is used to fabricate micro pillar arrays.•The adhesion strength of the micro pillar arrays is improved about 17% by the ultrasonic agitation method.
Micro electroforming is widely used for fabricating micro metal devices in Micro Electro Mechanism System (MEMS). However, there is the problem of poor adhesion strength between micro electroforming layer and substrate. This dramatically influences the dimensional accuracy of the device. To solve this problem, ultrasonic agitation method is applied during the micro electroforming process. To explore the effect of the ultrasonic agitation on the adhesion strength, micro electroforming experiments were carried out under different ultrasonic power (0W, 100W, 150W, 200W, 250W) and different ultrasonic frequencies (0kHz, 40kHz, 80kHz, 120kHz, 200kHz). The effects of the ultrasonic power and the ultrasonic frequency on the micro electroforming process were investigated by polarization method and alternating current (a.c.) impedance method. The adhesion strength between the electroforming layer and the substrate was measured by scratch test. The compressive stress of the electroforming layer was measured by X-ray Diffraction (XRD) method. The crystallite size of the electroforming layer was measured by Transmission Electron Microscopy (TEM) method. The internal contact surface area of the electroforming layer was measured by cyclic voltammetry (CV) method. The experimental results indicate that the ultrasonic agitation can decrease the polarization overpotential and increase the charge transfer process. Generally, the internal contact surface area is increased and the compressive stress is reduced. And then the adhesion strength is enhanced. Due to the different depolarization effects of the ultrasonic power and the ultrasonic frequency, the effects on strengthening the adhesion strength are different. When the ultrasonic agitation is 200W and 40kHz, the effect on strengthening the adhesion strength is the best. In order to prove the effect which the ultrasonic agitation can improve the adhesion strength of the micro devices, micro pillar arrays were fabricated under ultrasonic agitation (200W, 40kHz). The experimental results show that the residual rate of the micro pillar arrays is increased about 17% by ultrasonic agitation method. This work contributes to fabricating the electroforming layer with large adhesion strength.
•No-till with straw cover (NTS) improved soil quality and soil water storage before sowing compared to conventional tillage.•NTS and no-till with plastic mulch increased grain yield and precipitation ...use efficiency through optimizing water use.•NTS is the best tillage practice for sustainable intensification of spring wheat production in semi-arid areas.
Drought is a major limiting factor for rainfed spring wheat production on the semiarid Loess Plateau of China. Suitable tillage practices are important for improving precipitation use efficiency (PUE), which is the ratio of grain yield to annual precipitation. To obtain a better understanding of the effects of conservation tillage practices on PUE on the semiarid Loess Plateau, PUE was divided into five steps: precipitation storage efficiency, farmland water consumption rate, ratio of transpiration to evapotranspiration, crop transpiration efficiency, and harvest index. Six tillage practices were assessed in this paper, including conventional tillage with no straw (T), no-till with straw cover (NTS), no-till with no straw (NT), conventional tillage with straw incorporated (TS), conventional tillage with plastic mulch (TP), and no-till with plastic mulch (NTP), based on a long-term experiment initiated in 2001. The impact of tillage practices on soil quality, soil water storage, soil evaporation, biomass yield, and grain yield of spring wheat were monitored in 2015 and 2016. The results show that NTS improved soil quality and soil water storage before sowing. No-till with plastic mulch and NTS increased evapotranspiration but decreased evaporation, thus optimizing precipitation storage efficiency, the farmland water consumption rate, the ratio of transpiration to evapotranspiration, and crop transpiration efficiency, which gave rise to greater aboveground dry matter accumulation and more dry matter accumulation in grain. As a result, grain yield under NTS and NTP was significantly increased by 45 and 41 % compare to T, respectively, with corresponding improvements in PUE of 43 and 39 %. Therefore, both NTS and NTP have potential to substantially increase grain yield of spring wheat and PUE. However, for sustainable intensification in the long-run, NTS is the best combination of tillage and soil surface management for spring wheat production on the semi-arid Loess Plateau of China.
Cysticercosis pisiformis, a highly prevalent parasitic disease worldwide, causes significant economic losses in the rabbit breeding industry. Previous investigations have identified a novel microRNA, ...designated as novel-miR1, within the serum of rabbit infected with
. In the present study, we found that
-derived novel-miR1 was released into the rabbit serum via exosomes. Through computational analysis using TargetScan, miRanda, and PITA, a total of 634 target genes of novel-miR1 were predicted. To elucidate the functional role of novel-miR1, a dual-luciferase reporter assay was utilized and demonstrated that novel-miR1 targets rabbit Toll-like receptor 2 (TLR2). Rabbit peripheral blood lymphocytes (PBLCs) were transfected with novel-miR1 mimic and mimic NC, and the
experiments confirmed that novel-miR1 suppressed the expression of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 through the nuclear factor kappa B (NF-κB) pathway.
experiments demonstrated that novel-miR1 was significantly upregulated during the 1-3 months following infection with
rabbits. Notably, this upregulation coincided with a downregulation of TLR2, P65, pP65, TNF-α, IL-1β, and IL-6 in PBLCs. Collectively, these results indicate that the novel-miR1 derived from
inhibited the rabbits' immune response by suppressing the NF-κB-mediated immune response. This immune modulation facilitates parasite invasion, survival, and establishment of a persistent infection.
Chemotherapy combined with radiotherapy could reduce the risk of recurrence in early-stage extranodal NK/T lymphoma (ENKTL). However, the optimal combined chemotherapy regimen is still unknown. Our ...previous study reported that LVDP (
l
-asparaginase, etoposide, dexamethasone, and cisplatin) combined with radiotherapy was a potentially effective and safe treatment regimen for early-stage ENKTL. This study further validated the efficacy and safety of LVDP chemotherapy combined with radiation for early-stage ENKTL with more patients and longer follow-up. We retrospectively studied 112 patients with early-stage ENKTL from September 2010 to September 2019. All patients received the LVDP regimen, and 101 of them received radiotherapy. The patients’ characteristics, treatment responses, survival outcomes, prognostic factors, and toxicities were analyzed. The median follow-up was 60 months (range, 4 to 117). All patients received median 4 cycles of the LVDP chemotherapy. At the end of therapy, the objective response rate and complete response rate were 88.3% and 77.6%, respectively. The 3- and 5-year OS were 79.6% and 73.2%, and the 3- and 5-year PFS were 75.4% and 71.6%, respectively. Among them, the LVDP regimen combined with radiotherapy yielded more favorable treatment outcomes (the 3-year OS and PFS were 83.1% and 80.8%). The most common severe hematologic toxicity was leukopenia (25% grade 3/4), and the most common severe non-hematologic toxicity was increased transaminase (4.5% grade 3/4). No pancreatitis or treatment-related death occurred. The LVDP regimen combined with radiotherapy had a good therapeutic response and long-term survival with tolerable toxicity for patients with early-stage ENKTL.
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