In 2014, antimicrobial drug-resistant Campylobacter jejuni sequence type 6964 emerged contemporaneously in poultry from 3 supply companies in the North Island of New Zealand and as a major cause of ...campylobacteriosis in humans in New Zealand. This lineage, not previously identified in New Zealand, was resistant to tetracycline and fluoroquinolones. Genomic analysis revealed divergence into 2 major clades; both clades were associated with human infection, 1 with poultry companies A and B and the other with company C. Accessory genome evolution was associated with a plasmid, phage insertions, and natural transformation. We hypothesize that the tetO gene and a phage were inserted into the chromosome after conjugation, leaving a remnant plasmid that was lost from isolates from company C. The emergence and rapid spread of a resistant clone of C. jejuni in New Zealand, coupled with evolutionary change in the accessory genome, demonstrate the need for ongoing Campylobacter surveillance among poultry and humans.
Campylobacteriosis is the most commonly notified foodborne disease in New Zealand and poultry meat is the major source for human infection. Carcasses and portions were sampled from key points along ...primary and secondary processing chains of three New Zealand poultry processors to determine the impact of processing steps on Campylobacter concentrations. Primary processing reduced Campylobacter concentrations on carcasses by almost 6-log; the biggest reduction was achieved by the spinchill, followed by the scald step. Significant plant differences in the degree of Campylobacter reduction were also observed at these steps. The spinchill and final acidified sodium chlorite wash resulted in carcasses with low-to-no levels of Campylobacter regardless of concentrations at prior steps. A similar study was conducted at primary processing for one plant in 2013; significant improvements in Campylobacter mitigation since 2013 were noted. Campylobacter concentrations from final product from secondary processing were higher than concentrations at the end of primary processing. Drumsticks had lower Campylobacter concentrations than other portion types. Skin removal from product did not consistently result in product with lower Campylobacter concentrations. Results identify key areas to target for further reduction of Campylobacter on poultry meat, and provide a benchmark to compare the efficacy of future interventions.
•Primary processing reduced Campylobacter concentrations on carcasses by ∼6-log.•Campylobacter was not recovered from 76% of samples at the end of primary processing.•Campylobacter concentrations were higher on product at secondary processing.•Drumsticks had lower Campylobacter concentrations than all other portion types.•Skin removal did not result in lower Campylobacter concentrations on portions.
The movements of backyard poultry and wild bird populations are known to pose a disease risk to the commercial poultry industry. However, it is often difficult to estimate this risk due to the lack ...of accurate data on the numbers, locations, and movement patterns of these populations. The main aim of this study was to evaluate the use of three different data sources when investigating disease transmission risk between poultry populations in New Zealand including (1) cross-sectional survey data looking at the movement of goods and services within the commercial poultry industry, (2) backyard poultry sales data from the online auction site TradeMe®, and (3) citizen science data from the wild bird monitoring project eBird.
The cross-sectional survey data and backyard poultry sales data were transformed into network graphs showing the connectivity of commercial and backyard poultry producers across different geographical regions. The backyard poultry network was also used to parameterise a Susceptible-Infectious (SI) simulation model to explore the behaviour of potential disease outbreaks. The citizen science data was used to create an additional map showing the spatial distribution of wild bird observations across New Zealand. To explore the potential for diseases to spread between each population, maps were combined into bivariate choropleth maps showing the overlap between movements within the commercial poultry industry, backyard poultry trades and, wild bird observations.
Network analysis revealed that the commercial poultry network was highly connected with geographical clustering around the urban centres of Auckland, New Plymouth and Christchurch. The backyard poultry network was also a highly active trade network and displayed similar geographic clustering to the commercial network. In the disease simulation models, the high connectivity resulted in all suburbs becoming infected in 96.4 % of the SI simulations. Analysis of the eBird data included reports of over 80 species; the majority of which were identified as coastal seabirds or wading birds that showed little overlap with either backyard or commercial poultry.
Overall, our study findings highlight how the spatial patterns of trading activity within the commercial poultry industry, alongside the movement of backyard poultry and wild birds, have the potential to contribute significantly to the spread of diseases between these populations. However, it is clear that in order to fully understand this risk landscape, further data integration is needed; including the use of additional datasets that have further information on critical variables such as environmental factors.
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