Enzymes in Plant Metabolism of PCBs and PAHs Chroma, L.; Mackova, M.; Kucerova, P. ...
Acta biotechnologica,
01/2002, Letnik:
22, Številka:
1-2
Journal Article, Conference Proceeding
Recenzirano
Odprti dostop
Recently it has been shown that plants are able to transform polychlorinated biphenyls (PCBs) as well as polycyclic aromatic hydrocarbons (PAHs), but the knowledge of enzymes involved in these ...metabolic processes is limited. Plant peroxidases generally play an important role in plant metabolism. On the other hand, cytochrome P450 is involved in the detoxification of various xenobiotics in the cells of higher organisms. In this work, several in vitro cultures of different plant species were screened for their ability to transform PCBs or PAHs, and compared regarding their total extra‐ and intracellular peroxidase activity. Cultures with good transformation ability exhibited in the presence of xenobiotics the same or higher levels of peroxidases as the controls incubated without contaminants. Cultures with markedly lower peroxidase activity exhibited also lower PCB/PAH conversion in the presence of PCBs/PAHs. It was attempted to identify lignin peroxidase and Mn‐peroxidase in plants, originally described in white rot fungi to be responsible for the degradation of PCBs and other environmental pollutants. In addition to different types of peroxidases, RBBR oxidase was also detected in plants. The decolourisation of RBBR during the growth on agar plates was used as a rough screening method for plant cells able to metabolise PCBs/PAHs efficiently. The exact type of transformation reaction (peroxidative or oxidative) was studied using various inhibitors and inducers of peroxidases and cytochrome P450. It was shown that both enzymatic systems are partially involved in the detoxification mechanism of chosen xenobiotics in plants.
Based on \((2712.4\pm14.1)\times10^{6}~\psi(2S)\) events, 7.9 fb\(^{-1}\) \(\psi(3773)\) data, and 0.8 fb\(^{-1}\) off-resonance data samples collected with the BESIII detector, we measure the ...branching fraction of \(\psi(2S)\rightarrow\gamma\pi^{0}\) and \(e^{+}e^{-}\rightarrow\gamma\pi^{0}\) form factor at momentum transfers \(Q^{2}\sim13\) GeV\(^{2}\). The \(e^{+}e^{-}\rightarrow\gamma\pi^{0}\) cross section is fitted with considering the interference between the \(\psi(2S)\) and continuum amplitudes and two solutions are found, \({\cal B}=3.74\times10^{-7}\) with \(\phi=3.93\) rad and \({\cal B}=7.87\times10^{-7}\) with \(\phi=2.08\) rad. Here, \({\cal B}\) is the branching fraction of \(\psi(2S)\rightarrow\gamma\pi^{0}\) and \(\phi\) is the relative phase angle between the \(\psi(2S)\) and continuum amplitudes. Due to insufficient off-resonance data, the branching fraction \({\cal B}(\psi(2S)\rightarrow\gamma\pi^{0})\) is determined to be in the range \(2.7, 9.7\times10^{-7}\) within one standard deviation of the contour region.
We present a measurement of the integrated luminosity of \(e^+e^-\) collision data collected with the BESIII detector at the BEPCII collider at a center-of-mass energy of \(E_{\rm cm} = 3.773\)~GeV. ...The integrated luminosities of the data sets taken from December 2021 to June 2022, from November 2022 to June 2023, and from October 2023 to February 2024 are determined to be \(4.995 \pm 0.019\)~fb\(^{-1}\), \(8.157 \pm 0.031\)~fb\(^{-1}\), and \(4.191 \pm 0.016\)~fb\(^{-1}\), respectively, by analyzing large angle Bhabha scattering events. The uncertainties are dominated by systematic effects and the statistical uncertainties are negligible. Our results provide essential input for future analyses and precision measurements.
The branching fraction of \(D^+\to K_{S}^{0} \pi^{0}e^+\nu_e\) is measured for the first time using \(7.93~\mathrm{fb}^{-1}\) of \(e^+e^-\) annihilation data collected at the center-of-mass energy ...\(\sqrt{s}=3.773\)~GeV with the BESIII detector operating at the BEPCII collider, and is determined to be \({\mathcal B}\)(\(D^+\to K_S^0\pi^0e^+\nu_e\)) = \((0.881~\pm~0.017_{\rm stat.}~\pm~0.016_{\rm syst.})\)\%. Based on an analysis of the \(D^+\to K_S^0\pi^0e^+\nu_e\) decay dynamics, we observe the \(S\text{-}{\rm wave}\) and \(P\)-wave components with fractions of \(f_{S\text{-}{\rm wave}}\) = \((6.13~\pm~0.27_{\rm stat.}~\pm ~0.30_{\rm syst.})\%\) and \(f_{\bar K^{*}(892)^0}\) = \((93.88~\pm~0.27_{\rm stat.}~\pm~0.29_{\rm syst.})\)\%, respectively. From these results, we obtain the branching fractions \({\mathcal B}\)(\(D^+\to (K_S^0\pi^0)_{S\text{-}{\rm wave}}~e^+\nu_e\)) = \((5.41~\pm~0.35_{\rm stat.}~\pm~0.37_{\rm syst.})\times10^{-4}\) and \({\mathcal B}\)(\(D^+\to \bar K^{*}(892)^0e^+\nu_e\)) = \((4.97~\pm~0.11_{\rm stat.}~\pm~0.12_{\rm syst.})\)\%. In addition, the hadronic form-factor ratios of \(D^{+} \to \bar {K}^{*}(892)^0e^+\nu_e\) at \(q^2=0\), assuming a single-pole dominance parameterization, are determined to be \(r_V=\frac{V(0)}{A_1(0)}= 1.43~\pm~0.07_{\rm stat.}~\pm~0.03_{\rm syst.}\) and \(r_2=\frac{A_2(0)}{A_1(0)}=0.72~\pm~0.06_{\rm stat.}~\pm~0.02_{\rm syst.}\).
By analyzing \((27.12 \pm 0.14)\times10^{8}\) \(\psi(3686)\) events accumulated with the BESIII detector, the decay \(\eta_{c}(2S) \to K^{+} K^{-} \eta\) is observed for the first time with a ...significance of \(6.2\sigma\) after considering systematic uncertainties. The product of the branching fractions of \(\psi(3686) \to \gamma\eta_{c}(2S)\) and \(\eta_{c}(2S) \to K^{+} K^{-} \eta\) is measured to be \(\mathcal{B}(\psi(3686) \to\gamma\eta_{c}(2S))\times \mathcal{B}(\eta_{c}(2S)\to K^{+} K^{-}\eta)=(2.39 \pm 0.32 \pm 0.34) \times 10^{-6}\), where the first uncertainty is statistical, and the second one is systematic. The branching fraction of \(\eta_{c}(2S)\to K^{+} K^{-}\eta\) is determined to be \(\mathcal{B}(\eta_{c}(2S)\to K^{+} K^{-}\eta) = (3.42 \pm 0.46 \pm 0.48 \pm 2.44) \times 10^{-3}\), where the third uncertainty is due to the branching fraction of \(\psi(3686) \to \gamma\eta_{c}(2S)\). Using a recent BESIII measurement of \(\mathcal{B} (\eta_{c}(2S) \to K^{+} K^{-}\pi^{0})\), we also determine the ratio between the branching fractions of \(\eta_{c}(2S) \to K^{+} K^{-}\eta\) and \(\eta_{c}(2S) \to K^{+} K^{-}\pi^{0}\) to be \(1.49 \pm 0.22 \pm 0.25\), which is consistent with the previous result of BaBar at a comparable precision level.
Using 10.1 fb\(^{-1}\) of \(e^+e^-\) collision data collected by the BESIII detector with center-of-mass energies between 4.15 GeV and 4.30 GeV, we search for the decays ...\(X(3872)\to\pi^0\pi^0\chi_{c1,2}\), where the \(X(3872)\) is produced in \(e^+e^-\to\gamma X(3872)\). No evidence above \(3\sigma\) is found for either decay. Upper limits at the \(90\%\) C.L. on the branching fractions of \(X(3872)\to\pi^0\pi^0\chi_{c1,2}\) normalized to the branching fraction of \(X(3872)\to\pi^+\pi^-J/\psi\) are set to be \(\mathcal{B}(X(3872)\to\pi^0\pi^0\chi_{c1})/\mathcal{B}(X(3872)\to\pi^+\pi^-J/\psi) < 1.1\) and \(\mathcal{B}(X(3872)\to\pi^0\pi^0\chi_{c2})/\mathcal{B}(X(3872)\to\pi^+\pi^-J/\psi) < 0.5\), taking into account both statistical and systematic uncertainties.
Based on a sample of \((2712.4\pm14.3)\times10^6\;\psi(3686)\) events collected with the BESIII detector, a partial wave analysis of the decay \(\psi(3686)\to\Lambda\bar\Sigma^0\pi^0+c.c.\) is ...performed to investigate \(\Lambda^*\) and \(\Sigma^*\) resonances in the \(\pi^0\bar{\Sigma}^0\) and \(\pi^0\Lambda\) invariant mass distributions. Significant contributions are found from the \(\Lambda(1405)\), \(\Lambda(1520)\), \(\Lambda(1600)\), \(\Lambda(1670)\), \(\Lambda(1690)\), \(\Lambda(1800)\), \(\Lambda(1890)\), \(\Lambda(2325)\), \(\Sigma(1385)\), \(\Sigma(1660)\), \(\Sigma(1670)\), \(\Sigma(1750)\), and \(\Sigma(1910)\). The masses, widths, and production branching fractions for each component are determined. In addition, the branching fraction of \(\psi(3686)\to\Lambda\bar\Sigma^0\pi^0+c.c.\) is measured to be \((1.544\pm0.013\pm0.069)\times10^{-4}\) for the first time, where the first uncertainty is statistical and the second systematic.
By analyzing a data sample of \(e^+e^-\) collisions with center-of-mass energy \(\sqrt{s}=3.773\) GeV, corresponding to an integrated luminosity of \(7.9~\rm {fb}^{-1}\) collected with the BESIII ...detector operating at the BEPCII collider, we study semileptonic decays of the \(D^{0(+)}\) mesons into the axial-vector meson \(b_1(1235)\) via the decay \(b_1(1235)\to \omega\pi\). The decay \(D^0\to b_1(1235)^-e^{+}\nu_{e}\) is observed with a significance of 5.2\(\sigma\) after considering systematic uncertainty, while evidence for the decay \(D^+\to b_1(1235)^0 e^+\nu_e\) is obtained with a 3.1\(\sigma\) significance. The product branching fractions are determined to be \({\mathcal B}(D^0\to b_{1}(1235)^-e^{+}\nu_{e})\times {\mathcal B} (b_1(1235)^-\to \omega \pi^-) = (0.72\pm0.18^{+0.06}_{-0.08})\times10^{-4}\) and \({\mathcal B}(D^+\to b_{1}(1235)^0e^{+}\nu_{e})\times {\mathcal B} (b_1(1235)^0~\to \omega \pi^0) = (1.16\pm0.44\pm0.16)\times10^{-4}\), where the first uncertainties are statistical and the second systematic. The ratio of their partial decay widths is determined to be \(\frac{\Gamma(D^0\to b_{1}(1235)^-e^{+}\nu_{e})}{2\Gamma(D^+\to b_{1}(1235)^0e^{+}\nu_{e})}=0.78\pm0.19^{+0.04}_{-0.05}\), which is consistent with unity, predicted by isospin invariance, within uncertainties.
Using \(e^+e^-\) collision data collected with the BESIII detector at the BEPCII collider at center-of-mass energies between 3.510 and 4.914GeV, corresponding to an integrated luminosity of 25 ...fb\(^{-1}\), we measure the Born cross sections for the process \(e^+e^-\to K^-\bar{\Xi}^+\Lambda/\Sigma^{0}\) at thirty-five energy points with a partial-reconstruction strategy. By fitting the dressed cross sections of \(e^+e^-\to K^-\bar{\Xi}^+\Lambda/\Sigma^{0}\), evidence for \(\psi(4160) \to K^{-}\bar\Xi^{+}\Lambda\) is found for the first time with a significance of 4.4\(\sigma\), including systematic uncertainties. No evidence for other possible resonances is found. In addition, the products of electronic partial width and branching fraction for all assumed resonances decaying into \(K^{-}\bar\Xi^{+}\Lambda/\Sigma^{0}\) are determined.
We perform the first investigation of the process \(e^{+}e^{-}\to K^+K^-\psi(2S)\) and report its Born cross sections over a range of center-of-mass energies from 4.699 to 4.951~GeV. The measurements ...are carried out using several partial reconstruction techniques using data samples collected by the BESIII detector with a total integrated luminosity of 2.5~fb\(^{-1}\). We search for new tetraquark candidates \(Z_{cs}^\pm\) in the decays \(Z_{cs}^\pm\to K^\pm\psi(2S)\). No significant \(Z_{cs}^\pm\) signals are observed.
