Indiscriminate and injudicious use of antibiotics in layer farms is a common practice of Bangladesh for the compensation of Poor management practices and ignorance. Despite this scenario, there is no ...published documentation on antibiotic usage pattern and farm management practices in layer farms. This study was undertaken to understand the farmers' perspective in small-scale layer farms regarding antibiotics usage and farm management.
A questionnaire survey was conducted in 120 small-scale layer farms of Mymensingh district during January-February 2017. We only considered farms in production. Data were analyzed on antibiotic usage, purpose, egg management, understanding of antibiotic residue, withdrawal period, and other issues.
Among 120 farmers, about 94.16% of farmers are using antibiotics without respecting the withdrawal period. Only 39.1% of farmers possess knowledge of residues. In our surveyed farms, 91.83% of farmers are not practicing egg washing before supplying to the market and 52.67% of farmers are unaware of cleaning and disinfection of egg tray. Ten different types of antibiotics of seven classes have found in the survey. Most antibiotics are in the Watch (49%) and Reserve (8%) groups according to the WHO AWaRe categorization and 73% antibiotics are critically important for human medicine and are considered as last resort.
This study found that due to the lack of knowledge and poor management, farmers consider using antibiotics as the most effective practices to control disease and enhancement of egg production. These indiscriminate uses of antibiotics are responsible for antibiotic residual and resistance problem. Here, we also provide some suggestion and guidelines to improve management practices to minimize the emerging problems of antimicrobial resistance through small-scale layer farms.
The study is aimed to understand the antibacterial sensitivity of native and Indian varieties of garlic (
) and ginger (
) crude extracts against multidrug-resistant (MDR) poultry pathogen (
and
...sp.).
Thin layer chromatography (TLC) is used to identify the target spices' bioactive antibacterial compounds. MDR
and
sp. were isolated from poultry. The TLC-Bioautography technique was applied to explore the antibacterial potentiality of garlic and ginger.
Inhibitory activities of garlic were Zone of inhibition (ZI) = 14.03 ± 0.15 mm and 19.70 ± 0.36 mm, Minimum inhibitory concentration (MIC): 0.625 and 0.325 mg/ml, and ginger were ZI = 14.63 ± 0.30 mm and 11.56 ± 0.51mm, MIC: 9.0 mg/ml against
sp., respectively. Two bands of garlic (
value = 0.31 and 0.50) and one band of ginger (
value = 0.71) showed inhibitory potential in TLC-Bioautography against both MDR isolates.
Garlic and ginger were effective against MDR
and
sp
These spices could be a suitable alternative during the antibiotic void.
The imprudent use of antibiotics increases the environmental microflora's resistance to various drugs, particularly antibiotics. Prescription data is crucial for understanding antibiotic usage ...frequency and dosage. This health-focused study aims to analyze antibiotic prescription patterns in human and veterinary practices to identify emerging trends in environmental antibiotic resistance.
For this survey, A total of 6550 prescriptions were randomly collected from hospitals and pharmacies in Mymensingh sadar upazila, Bangladesh, between August and October 2022. Of these, 5123 (78 %) were for human cases and 1427 (22 %) for veterinary purposes. Photos of the prescriptions were taken and analyzed to understand prescribing habits. Additionally, 30 water samples from rivers, ponds, sewage, and households in Mymensingh City Corporation were collected to assess environmental antibiotic levels and resistance patterns of microorganisms.
The analysis showed that Cephalosporins were the most prescribed antibiotics, found in 570 (56.27 %) of human prescriptions and 230 (42.99 %) of veterinary prescriptions. Aminoglycosides had the lowest frequency, with 13 (1.2 %) for humans and 46 (8.6 %) for animals. Macrolides (12.24 %), carboxylic acids (1.87 %), and rifamycins (1.28 %) were only found in human prescriptions, while sulfa drugs (10.84 %), tetracyclines (5.42 %), and combinations of antibiotics (14.77 %) were only in animal prescriptions. Quinolones were prescribed 4.06 times more for humans, while aminoglycosides were used 3.54 times more for animals. Environmental samples showed E. coli had the highest resistance (MAR Value: 0.625) against eight antibiotics.
This study illuminates the human-animal prescription patterns that are influenced by environmental factors which drive antibiotic stewardship in Bangladesh. It is imperative for practitioners to exercise caution and adhere to guidelines when prescribing antibiotics, both in human and veterinary practices, given the alarming trend of antibiotic resistance. Additionally, measures must be taken to restrict the influx of antibiotics residue into the environment.
To analyze the presence of selective antibiotic residues (oxytetracycline, amoxicillin, and ciprofloxacin) in milk during the antibiotic treatment course, and to evaluate the thermal effect on ...antibiotics residual status in milk of antibiotic-treated cows.
The raw fresh milk was collected from 18 lactating cows before antibiotics treatment, which were brought to the veterinary hospital and suffered from either mastitis, foot and mouth disease, fever, local wound, or non-specific diarrhea, and so on. Out of the 18 lactating cows, six were treated with oxytetracycline, six were treated with amoxicillin, and six were treated with ciprofloxacin parenterally. Milk samples were also collected at 2nd day during treatment and final collection was done after maintaining the withdrawal period. Since milk is heated before consumption, it was boiled at 100°C for 20 min to evaluate the thermal effect on antibiotics residual status. Thin-layer chromatography was done for screening of antibiotics residue before and after boiling of the milk.
At day 0 (before antibiotic treatment), no antibiotics (oxytetracycline, ciprofloxacin and amoxicillin) residue was detected in raw milk of antibiotic treated cows. In contrast, on day 2 (during antibiotic treatment), 100% raw milk samples showed positive for antibiotics residue. After boiling, all milk samples showed positive for such specific antibiotics residue. On the other hand, no antibiotics residues were detected on day 9, which indicates the completion of the withdrawal period of the respective antibiotic. The intensities of bands for antibiotic on thin-layer chromatography plate of antibiotic residues in milk samples (oxytetracycline, amoxicillin, and ciprofloxacin) expressed that the respective antibiotic residual status was higher in the boiled milk compare to the raw milk.
Proper maintenance of withdrawal period after antibiotic treatment would minimize the risk of antibiotic residues in milk, and boiling does not change these specific antibiotics residual status in milk. Therefore, awareness regarding the proper maintenance of withdrawal period after antibiotic treatment in lactating cows is one of the best strategies that may positively reduce the risk of antimicrobial drugs residue in milk.
Bovine subclinical mastitis (SCM) is highly prevalent among dairy cattle. A cross-sectional study was conducted in Bangladesh to evaluate the performance of electric conductivity (EC) and total ...dissolved solids (TDS) tests for the detection of SCM.
We randomly selected 108 milk samples from cows of different breeds in the primary milk-producing region of Pabna and Sirajgonj districts of Bangladesh. Samples were subjected to the California mastitis test (CMT), white side test (WST), electric conductivity (EC), TDS, and culture. A cow was considered positive for SCM if it tested positive in CMT, WST, and culture, whereas a cow was considered negative for SCM if it tested negative in all three methods. These gold standards have been used to evaluate the performance of the EC and TDS tests. The optimal EC and TDS cutoff values for the detection of SCM were determined using the "optimal cutoff" function in R version 4.3.1.
The optimal EC cutoff value for SCM detection was found to be 6159 μS/cm or 6.16 mS/cm. A positive likelihood ratio (LR
) of 31.2 and an area under the curve (AUC) of 0.905 were obtained for this cutoff value. The optimal cutoff value for TDS was 3100 mg/L of milk, which resulted in a positive LR
of 45.5 and an AUC of 0.924.
To the best of our knowledge, this is the first study to evaluate the performance of EC and TDS tests in detecting SCM in Bangladesh. These results suggest that EC and TDS tests, which are inexpensive, rapid, and easy to conduct, can effectively detect SCM at the farm level.
In recent years, lead (Pb) has arisen as a foremost contaminant due to overpopulation, rapid industrialization, and expansion that could contaminate the human food chain. However, the correlation ...between the environmental contamination of Pb and its spatial transfer to the dairy products is still unmapped. In this paper, we intend to evaluate the concentration of environmental Pb and its spatial distribution in dairy feed and products in Narayangonj, a highly polluted district of Bangladesh.
A total of 125 samples of soil, water, forage, and milk were collected from five upazilas (Narayangonj Sadar, Bondor, Rupgonj, Araihazar, and Sonargoan) of Narayangonj. The samples were digested by acid digestion, and Pb was detected by flame atomic absorption spectrophotometer and graphite furnace atomic absorption spectrometer. The bioconcentration factor of the samples was also calculated.
In this study, 25% of the soil, 20% of the water, 5% of the forage, and 2% of the milk samples contained Pb at a variable level. Among the environmental samples, the highest concentration (26-39 μg/kg) of Pb was detected in the soil, followed by in the water (0.023-0.059 μg/kg) and forage (0.017-0.035 μg/kg). The contamination (0.041-0.068 μg/kg) in the milk, however, was lower than the soil but higher than the water and forage. The concentration of Pb in all the samples was within a safer limit. None of the forage samples was the potential bioaccumulator.
Although no linear correlation was established between the environmental samples, forage, and milk, the study identifies the potentials of the spatial distribution of Pb from the environment to the dairy feed and products. Therefore, feasible procedures should be adapted to cease the residue to the human food chain.
ABSTRACT
This study announces the sequence of a multidrug-resistant
Escherichia coli
MAHK_SCM_BAU_30A strain isolated from bovine subclinical mastitis milk in 2022 in Bangladesh. Our assembled genome ...had a length of 4,884,948 bp, three plasmids, two CRISPR arrays, five prophages, 51 predicted antibiotic resistance, and 72 predicted virulence factor genes.
A retrospective survey was designed to determine the prevalence and factors involved in surgical cases at the Veterinary Teaching Hospital (VTH), Bangladesh Agricultural University from June 2014 to ...June 2017. In total, 1042 surgical cases of food animal (large ruminants: n=564, and small ruminants: n=493) and 26 non-food animal (monogastric animal) were recorded from patient register book and case recording card. Data were analyzed by Epi Info TM software and frequencies were calculated for different variables using Statistical Package for Social Science (SPSS) software. In large ruminant, hernia (16.13%) ranked top (90% umbilical and 10% lateral) followed by fracture (14.89%), abscess (14.54%), umbilical myasis (10.46%), atresia ani (5.85%) and naval ill (4.07%). Among the reported cattle, 87.41% were crossbred and 12.59% were indigenous. Calf, heifer and adult cattle were 55.32, 10.29 and 34.39% respectively. In small ruminants, castration (32.94%; n=138) ranked top followed by myasis (10.55%) naval ill (10.31%), abscess (7.44%), dystocia (6.24%) and urolithiasis (5.49%). Based on surgical classification in large and small ruminants, 37.56 and 42.50% were reported for general surgery, whereas 28.71 and 7.15% for congenital, 11.18 and 13.12% for gynecological and 2.65 and 37.23 % for andrological problems, respectively. Male and female ratio was 1:1.31 and 2:1 respectively in large and small ruminants, respectively. This study emphasizes the factors related to successful surgical cases management at VTH. The results may help in controlling surgical related cases in Mymensingh region of Bangladesh.
Objective: Subclinical mastitis (SCM) in cows is a major challenge in dairying not only in disease management but also in financial issues. The objective of this study is to predict an innovative and ...sustainable approach for the identification of bovine sub-clinical mastitis using machine learning techniques targetting milk biomarkers like electric conductivity and total dissolved
solids.
Materials and Methods: The field data on milk electric conductivity (EC) and total dissolved solids (TDS) will be assimulated and connected to a central network system for cross-matching with the library database to predict the result. The cut-off value of milk EC and TDS as standerdize previously would be the exploratory data in machine learning and the output of which need to be translated into language by artificial intelegnce.
Results: The optimal EC cutoff value for SCM detection in dairy cows was standardize previously as 6159 μS/cm or 6.16 mS/cm and TDS as 3100 mg/l of milk by examining milk from 108 suspected animals. An automated machine learning based message (study in progress) is assuming to notify the onset of SCM and thus deliver the information to the veterinary/ regulatory authority or dairy owners to predict the severity of SCM and taking necessary actions.
Conclusion: This model is assuming to serve the one-step veterinary diagnostic service for bovine subclinical mastitis in Bangladesh.
The black soldier fly (BSF) Hermetia illucens (L.) (Diptera: Stratiomyidae) is a “crown jewel” in waste management because it rapidly bioconverts organic streams into nutrient-rich biomass. ...Bangladesh's inefficient waste management has caused serious environmental issues due to the massive amounts of organic waste, mainly livestock manures and municipal organic waste, generated daily. We need reliable BSF to maximize our organic resources. To establish a long-term, stable BSF colony, we closely examined the seasonal abundance and life cycle features of BSF raised on municipal garbage and poultry manure in a regional natural environment in the lab. This investigation lasted two years (2019–2020). Season and raising substrates affected wild BSF egg clutches and life cycles. Organic waste dumps captured the most egg clutches in rainy seasons and the fewest in cooler months. Municipal rubbish dumps consistently produced wilder BSF egg clutches than poultry manure dumpsites. The BSF larvae grew slower in winter and faster in the rainy season, independent of substrate. Winter was optimal for adult BSF longevity in all organic streams. Winter cultivated wild BSF on two organic streams exhibited fewer egg clutches per female, shorter and lighter prepupae, and lower adult ecolosion rates than wetter months. While BSF given municipal organic wastes had the highest adult ecolosion rates, BSF fed poultry manure had more variation in prepupae length and weight, adult lifespan, and egg clutches. Larval development was faster with poultry manure BSF than with municipal organic waste. The protein and ash content of prepupae rearing on poultry manure exceeded those of prepupae on urban organic waste, although fat content was equivalent. These findings indicate that both types of organic streams may be suitable substrates for BSF larvae for trash treatment.
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