The ever growth of urban population has caused the increase of energy demand. This situation challenges the global public to find a way to produce energy in a secure, environmentally friendly, ...flexible, renewable and sustainable energy transformation system. One of the most promising technologies that can over-come most of the challenges is the district energy network. This paper focuses on best practice project in Latvia – fully renewable district heating system assisted by solar collector system with thermal storage tank and woodchip boilers. The article demonstrates solar impact to district heating system (DHS) in the framework of the current situation of DHS in Latvia by creating simulation in TRNSYS and validation with real system operation data. Real operational data show that the heat produced by solar collector system with thermal energy storage (TES) per year will cover up to 20% (10933,2 MWh) of the heating demand in under local climate conditions. The TRNSYS simulations concluded that the contribution of this type of solar system integrated into the DHS could be increased up to 50%, if the difference between consumption and heat produced by solar collectors were to be increased during the summer months, this could be achieved, for example, by increasing the solar collector field area.
Electricity generation from photovoltaic (PV) in Latvia is currently below the necessary capacity required to contribute to achieving climate neutrality by 2050. However, photovoltaic offer ...households and companies the opportunity to align their electricity consumption with environmental goals while supporting the European Green Deal objectives. In recent years, there was a growing interest in the installation of photovoltaic microgenerators in Latvia. By June 2023, the total number of households microgenerators connected to the grid has reached 15,000, boasting a collective production capacity exceeding 120 MW. This significant increase is in stark contrast to the situation 5 years ago when Latvia had a mere 3 MW of photovoltaic capacity. Several factors contributed to this growth, including a surge in electricity prices, at times surging more than tenfold, and the implementation of renewable energy (RE) use support measures to facilitate the acquisition of RE systems. Furthermore, 2023 witnessed numerous amendments to Latvia’s energy policy documents and the introduction of the concept of citizen energy communities (CEC), indicating a growing emphasis on harnessing solar energy potential in the country. However, it remains crucial to base these endeavors on accurate, economically viable information regarding solar technologies, their costs and their anticipated long-term outcomes. This manuscript aims to provide an overview of the grid-connected potential of rooftop photovoltaic systems within a Latvian urban setting. Through extensive research, a model has been developed, employing a thoroughly tested simulation program for evaluating the generation capacity of photovoltaic systems. This model considers real electricity consumption data, the existing infrastructure and economic factors. The findings affirm the technical and economic viability of urban rooftop photovoltaic systems within the Latvian context. It has been established that the implementation of such citizen energy communities energy systems holds significant potential. These systems have the potential to be a promising solution for future electricity generation, addressing some of the demands while relieving strain on external power grids. However, the full potential can only be realized with improved infrastructure, and the system’s profitability is heavily contingent on market dynamics and political conditions. This study may also be applicable to other photovoltaic systems facing similar climate conditions.
An academic integrity policy usually specifies the university’s ethical principles and values, the forms of appropriate academic behaviour, the penalties for academic malpractice and the procedures ...for handling policy violations. However, the policy does not itself create academic integrity, but needs to be consistently and effectively communicated, implemented, and applied. This paper presents a case study investigating the extent to which students at Riga Technical University (Latvia) are informed about the university’s academic integrity policy. This quantitative research was performed by surveying two groups of respondents: domestic students in the first year of their bachelor’s studies and the heads of the corresponding study programmes. The results revealed a low level of students’ awareness of the university policy on academic integrity and a lack of systematic institutional approach to promoting academic integrity. The study results also indicated that students with a higher awareness of the university policy on academic integrity were less tolerant towards violations and perceived academic integrity as an essential aspect for both the academic community and student life. These findings establish a foundation for a more extensive discussion of academic integrity issues in Latvia. They can also serve as impetus for similar studies in other Latvian universities, as well as research taking an international comparative perspective.
Academic integrity determines the trust that society has in the quality of education and the results of scientific research. More broadly, it influences honesty, respect for ethical principles, and ...the fair behaviour of society members. Accordingly, higher education institutions should have clear, transparent and well-communicated policies to defend academic integrity among all stakeholders. Taking into account the worldwide dependence on digital technologies, online communication channels should be also used for this purpose. Using qualitative content analysis, this paper aims to investigate how academic integrity is framed by Latvian and Lithuanian state-financed universities in online public spaces in relation to the criteria of an exemplary academic integrity policy. Given the limited content of publicly available university documents, the research findings indicated a poor correspondence to the mentioned criteria.
Pronectins™ are a new class of fibronectin-3-domain 14th-derived (14Fn3) antibody mimics that can be engineered as bispecific T cell engager (BTCE) to redirect immune effector cells against cancer. ...We describe here the in vitro and in vivo activity of a Pronectin™ AXL-targeted first-in-class bispecific T cell engager (pAXLxCD3ε) against Epithelial Ovarian Cancer (EOC).
pAXLxCD3ε T-cell mediated cytotoxicity was evaluated by flow cytometry and bioluminescence. pAXLxCD3ε mediated T-cell infiltration, activation and proliferation were assessed by immunofluorescence microscopy and by flow cytometry. Activity of pAXLxCD3ε was also investigated in combination with poly-ADP ribose polymerase inhibitors (PARPi). In vivo antitumor activity of pAXLxCD3ε was evaluated in immunocompromised (NSG) mice bearing intraperitoneal or subcutaneous EOC xenografts and immunologically reconstituted with human peripheral blood mononuclear cells (PBMC).
pAXLxCD3ε induced dose-dependent cytotoxicity by activation of T lymphocytes against EOC cells, regardless of their histologic origin. The addition of PARPi to cell cultures enhanced pAXLxCD3ε cytotoxicity. Importantly, in vivo, pAXLxCD3ε was highly effective against EOC xenografts in two different NSG mouse models, by inhibiting the growth of tumor cells in ascites and subcutaneous xenografts. This effect translated into a significantly prolonged survival of treated animals.
pAXLxCD3ε is an active therapeutics against EOC cells providing a rational for its development as a novel agent in this still incurable disease. The preclinical validation of a first-in-class agent opens the way to the development of a new 14Fn3-based scaffold platform for the generation of innovative immune therapeutics against cancer.
A highly specific AXL-receptor targeted family of non-immunoglobulin, single domain protein binders (Pronectins™) have been isolated from three (3) synthetic libraries that employ the human scaffold ...of the 14th domain of Fibronectin III (14FN3) and evolutionary CDRs diversity of over 25 billion loop sequences. The three libraries, each containing diversity in two loops, were designed to expand upon a human database of more than 6000 natural scaffold sequences and approximately 3000 human loop sequences. We used a bioinformatic-based approach to maximize "human" amino acid loop diversity and minimize or prevent altogether CDR immunogenicity created by the use of mutagenesis processes to generate diversity. A combination of phage display and yeast display was used to isolate 59 AXL receptor targeted Pronectins with KD ranging between 2 and 100 nM. FACS analysis with tumor cells over-expressing AXL and the use of an AXL knock-out cell line allowed us to identify Pronectin candidates with exquisite specificity for AXL receptor. Based upon several in vitro cell-based tests, we selected the best candidate, AXL54, to further characterize its in vitro cancer cells killing activity. Finally, AXL54 was used to produce the first bi-specific T cell engager protein (AXL54 Pronectin-linker-scFV CD3), a "new in class" protein for further testing of its anti-tumor activity in vitro and in vivo.
Following publication of the original article 1, the author reported that the values for LV1, LV2, LV3 and LV4 in row “Website section” of Table 2 should be corrected as follows:
The article investigates the influence of different ventilation strategies on annual energy consumption and thermal comfort. The method proposed for the study is the analysis of the building model ...realized in the energy simulation software IDA Indoor Climate and Energy (IDA-ICE) 4.7 - a tool for dynamic simulation of thermal comfort, indoor air quality and energy consumption in buildings. The especial attention is paid to application of external shadings which is not popular in cold climate. Achieved results have shown operative temperature is below 26°C.
A new directed evolution method was used to enhance the thermostability of the wild-type GH11 xylanase 2 (known as BD-11) from Hypocrea jecorina (Trichoderma reesei). Both Look-Through Mutagenesis ...(LTM™), which is a method for rapidly screening selected positions in the protein sequence for amino acids that introduce favorable properties, and Combinatorial Beneficial Mutagenesis (CBM™), which is a method for identifying the best ensemble of individual mutations, were employed to enhance the stability of an enzyme that has been thoroughly engineered by various means during the past 20 years. A diverse set of novel mutations was discovered, including N71D, Y73G, T95G and Y96Q. When these mutations were combined into a single construct (Hjx-81), the purified protein was active even after heating at 100°C for 20 min. This time-effective method should be generally applicable for quickly improving the physico-chemical properties of other industrial and therapeutic enzymes in only several months time.
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