3D printing and nanotechnology have been two important tools in the development of therapeutic approaches for personalized medicine. More recently, their alliance has been improved in an effort to ...build innovative, versatile, multifunctional, and/or smart medical and pharmaceutical products. Therefore, an extensive review about scientific studies that ally 3D printing and nanomaterials in the development of new approaches for pharmaceutical and medical applications for the treatment and prevention of diseases is presented here. The articles are classified into five categories according to their main application: Cell growth and tissue engineering, antimicrobial, drug delivery, stimulus‐response, and theranostics. Semisolid extrusion, inorganic nanoparticles, and cell growth and tissue engineering are the most reported 3D printing technique, type of nanomaterial, and application, respectively. The increase in papers dedicated to these areas is also notable, especially in the 2019 and 2020, when semisolid extrusion became the most used technique, overcoming fused deposition modelling. In fact, this review highlights that the possibility of an alliance between 3D printing and nanotechnology for the production of multiscale materials is undoubtedly a great opportunity for knowledge and innovation in the pharmaceutical and medical area.
In this review, the alliance of nanotechnology and 3D printing as an approach for the development of innovative materials for pharmaceutical and biomedical applications is reported. This confluence brings about the opportunity to tailor the product format and opens the door to multifunctional products.
A Dataset for Breast Cancer Histopathological Image Classification Spanhol, Fabio A.; Oliveira, Luiz S.; Petitjean, Caroline ...
IEEE transactions on biomedical engineering,
2016-July, 2016-07-00, 2016-7-00, 20160701, 2016-07, Letnik:
63, Številka:
7
Journal Article
Recenzirano
Today, medical image analysis papers require solid experiments to prove the usefulness of proposed methods. However, experiments are often performed on data selected by the researchers, which may ...come from different institutions, scanners, and populations. Different evaluation measures may be used, making it difficult to compare the methods. In this paper, we introduce a dataset of 7909 breast cancer histopathology images acquired on 82 patients, which is now publicly available from http://web.inf.ufpr.br/vri/breast-cancer-database. The dataset includes both benign and malignant images. The task associated with this dataset is the automated classification of these images in two classes, which would be a valuable computer-aided diagnosis tool for the clinician. In order to assess the difficulty of this task, we show some preliminary results obtained with state-of-the-art image classification systems. The accuracy ranges from 80% to 85%, showing room for improvement is left. By providing this dataset and a standardized evaluation protocol to the scientific community, we hope to gather researchers in both the medical and the machine learning field to advance toward this clinical application.
•H. annuus was inoculated with PBB and AMF to remediate Ni contaminated saline soil.•Inoculation of PBB or PBB+AMF improved plant performance under SS, MS or SS+MS.•Microbes (singly and dually ...inoculated) decreased Ni and Na+ uptake under SS+MS.•PBB and AMF colonized the rhizosphere or roots of H. annuus under SS, MS or SS+MS.•Inoculation improves Ni phytostabilization, Na+ exclusion, Ni and Na+ detoxification.
Phytoremediation has been considered as a promising technique to decontaminate polluted soils. However, climatic stress particularly salinity, is a potential threat to soil properties and plant growth, thus restricting the employment of this technology. The aim of this study was to access the impact of microbial inoculation on phytoremediation of nickel (Ni) contaminated saline soils using Helianthus annuus. Salt resistant plant beneficial bacterium (PBB) Pseudomonas libanensis TR1 and arbuscular mycorrhizal fungus (AMF) Claroideoglomus claroideum BEG210 were used. Inoculation of P. libanensis alone or in combination with C. claroideum significantly enhanced plant growth, changed physiological status (e.g. electrolyte leakage, chlorophyll, proline and malondialdehyde contents) as well as Ni and sodium (Na+) accumulation potential (e.g. uptake and translocation factor of Ni and Na+) of H. annuus under Ni and salinity stress either alone or in combination. These results revealed that bioaugmentation of microbial strains may serve as a preferred strategy for improving phytoremediation of metal-polluted saline soils.
Dosage of local anesthetics (LAs) used for regional anesthesia in children is not well determined. In order to evaluate and come to a consensus regarding some of these controversial topics, The ...European Society of Regional Anaesthesia and Pain Therapy (ESRA) and the American Society of Regional Anesthesia and Pain Medicine (ASRA) developed a Joint Committee Practice Advisory on Local Anesthetics and Adjuvants Dosage in Pediatric Regional Anesthesia.
Representatives from both ASRA and ESRA composed the joint committee practice advisory. Evidence-based recommendations were based on a systematic search of the literature. In cases where no literature was available, expert opinion was elicited.
Spinal anesthesia with bupivacaine can be performed with a dose of 1 mg/kg for newborn and/or infant and a dose of 0.5 mg/kg in older children (>1 year of age). Tetracaine 0.5% is recommended for spinal anesthesia (dose, 0.07-0.13 mL/kg). Ultrasound-guided upper-extremity peripheral nerve blocks (eg, axillary, infraclavicular, interscalene, supraclavicular) in children can be performed successfully and safely using a recommended LA dose of bupivacaine or ropivacaine of 0.5 to 1.5 mg/kg. Dexmedetomidine can be used as an adjunct to prolong the duration of peripheral nerve blocks in children.
High-level evidence is not yet available to guide dosage of LA used in regional blocks in children. The ASRA/ESRA recommendations intend to provide guidance in order to reduce the large variability of LA dosage currently observed in clinical practice.
Aims
Brazil ranks high in the number of coronavirus disease 19 (COVID‐19) cases and the COVID‐19 mortality rate. In this context, autopsies are important to confirm the disease, determine associated ...conditions, and study the pathophysiology of this novel disease. The aim of this study was to assess the systemic involvement of COVID‐19. In order to follow biosafety recommendations, we used ultrasound‐guided minimally invasive autopsy (MIA‐US), and we present the results of 10 initial autopsies.
Methods and results
We used MIA‐US for tissue sampling of the lungs, liver, heart, kidneys, spleen, brain, skin, skeletal muscle and testis for histology, and reverse transcription polymerase chain reaction to detect severe acute respiratory syndrome coronavirus 2 RNA. All patients showed exudative/proliferative diffuse alveolar damage. There were intense pleomorphic cytopathic effects on the respiratory epithelium, including airway and alveolar cells. Fibrinous thrombi in alveolar arterioles were present in eight patients, and all patients showed a high density of alveolar megakaryocytes. Small thrombi were less frequently observed in the glomeruli, spleen, heart, dermis, testis, and liver sinusoids. The main systemic findings were associated with comorbidities, age, and sepsis, in addition to possible tissue damage due to the viral infection, such as myositis, dermatitis, myocarditis, and orchitis.
Conclusions
MIA‐US is safe and effective for the study of severe COVID‐19. Our findings show that COVID‐19 is a systemic disease causing major events in the lungs and with involvement of various organs and tissues. Pulmonary changes result from severe epithelial injury and microthrombotic vascular phenomena. These findings indicate that both epithelial and vascular injury should be addressed in therapeutic approaches.
The impacts of climate change on soil erosion may bring serious economic, social and environmental problems. However, few studies have investigated these impacts on continental scales. Here we ...assessed the influence of climate change on rainfall erosivity across Brazil. We used observed rainfall data and downscaled climate model output based on Hadley Center Global Environment Model version 2 (HadGEM2-ES) and Model for Interdisciplinary Research On Climate version 5 (MIROC5), forced by Representative Concentration Pathway 4.5 and 8.5, to estimate and map rainfall erosivity and its projected changes across Brazil. We estimated mean values of 10,437 mm ha
h
year
for observed data (1980-2013) and 10,089 MJ mm ha
h
year
and 10,585 MJ mm ha
h
year
for HadGEM2-ES and MIROC5, respectively (1961-2005). Our analysis suggests that the most affected regions, with projected rainfall erosivity increases ranging up to 109% in the period 2007-2040, are northeastern and southern Brazil. Future decreases of as much as -71% in the 2071-2099 period were estimated for the southeastern, central and northwestern parts of the country. Our results provide an overview of rainfall erosivity in Brazil that may be useful for planning soil and water conservation, and for promoting water and food security.
Purpose
This paper aims to investigate customers' motives to engage with electronic word of mouth (eWOM), as well as the effect of eWOM on customer behavior, both at the purchase and post-purchase ...stages.
Design/methodology/approach
A qualitative approach was adopted, consisting of 30 semi-structured interviews with Thai consumers. Thematic analysis was used to analyze the data.
Findings
Information validation, product evaluation, purchase and post-purchase validation are key motives for consumers to search for eWOM. Furthermore, eWOM quantity, eWOM credibility and attitudes toward eWOM play a critical role in evaluation of information usefulness and adoption. Moreover, five different types of shoppers were identified according to their purchase behavior after adapting eWOM: prompt shoppers, in-store shoppers, promotion shoppers, conservative shoppers and remedy shoppers.
Originality/value
While the implications of the adoption of eWOM have been explored in previous research, there is limited understanding in terms of the impacts of eWOM on the customer journey. This study addresses this research gap by investigating not only customers' motives to engage with eWOM but also its effect on their behavior at the purchase and post-purchase stages.
Transformers for Energy Forecast Oliveira, Hugo S; Oliveira, Helder P
Sensors (Basel, Switzerland),
08/2023, Letnik:
23, Številka:
15
Journal Article
Recenzirano
Odprti dostop
Forecasting energy consumption models allow for improvements in building performance and reduce energy consumption. Energy efficiency has become a pressing concern in recent years due to the ...increasing energy demand and concerns over climate change. This paper addresses the energy consumption forecast as a crucial ingredient in the technology to optimize building system operations and identifies energy efficiency upgrades. The work proposes a modified multi-head transformer model focused on multi-variable time series through a learnable weighting feature attention matrix to combine all input variables and forecast building energy consumption properly. The proposed multivariate transformer-based model is compared with two other recurrent neural network models, showing a robust performance while exhibiting a lower mean absolute percentage error. Overall, this paper highlights the superior performance of the modified transformer-based model for the energy consumption forecast in a multivariate step, allowing it to be incorporated in future forecasting tasks, allowing for the tracing of future energy consumption scenarios according to the current building usage, playing a significant role in creating a more sustainable and energy-efficient building usage.
Plant beneficial microbes (PBMs), such as plant growth-promoting bacteria, rhizobia, arbuscular mycorrhizal fungi, and
Trichoderma
, can reduce the use of agrochemicals and increase plant yield, ...nutrition, and tolerance to biotic–abiotic stresses. Yet, large-scale applications of PBM have been hampered by the high amounts of inoculum per plant or per cultivation area needed for successful colonization and consequently the economic feasibility. Seed coating, a process that consists in covering seeds with low amounts of exogenous materials, is gaining attention as an efficient delivery system for PBM. Microbial seed coating comprises the use of a binder, in some cases a filler, mixed with inocula, and can be done using simple mixing equipment (e.g., cement mixer) or more specialized/sophisticated apparatus (e.g., fluidized bed). Binders/fillers can be used to extend microbial survival. The most reported types of seed coating are seed dressing, film coating, and pelleting. Tested in more than 50 plant species with seeds of different dimensions, forms, textures, and germination types (e.g., cereals, vegetables, fruits, pulses, and other legumes), seed coating has been studied using various species of plant growth-promoting bacteria, rhizobia,
Trichoderma
, and to a lesser extent mycorrhizal fungi. Most of the studies regarding PBM applied
via
seed coating are aimed at promoting crop growth, yield, and crop protection against pathogens. Studies have shown that coating seeds with PBM can assist crops in improving seedling establishment and germination or achieving high yields and food quality, under reduced chemical fertilization. The right combination of biological control agents applied
via
seed coating can be a powerful tool against a wide number of diseases and pathogens. Less frequently, studies report seed coating being used for adaptation and protection of crops under abiotic stresses. Notwithstanding the promising results, there are still challenges mainly related with the scaling up from the laboratory to the field and proper formulation, including efficient microbial combinations and coating materials that can result in extended shelf-life of both seeds and coated PBM. These limitations need to be addressed and overcome in order to allow a wider use of seed coating as a cost-effective delivery method for PBM in sustainable agricultural systems.
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