To establish the experts consensus on the right heart function management in critically ill patients. The panel of consensus was composed of 30 experts in critical care medicine who are all members ...of Critical Hemodynamic Therapy Collaboration Group (CHTC Group). Each statement was assessed based on the GRADE (Grading of Recommendations Assessment, Development, and Evaluation) principle. Then the Delphi method was adopted by 52 experts to reassess all the statements. (1) Right heart function is prone to be affected in critically illness, which will result in a auto-exaggerated vicious cycle. (2) Right heart function management is a key step of the hemodynamic therapy in critically ill patients. (3) Fluid resuscitation means the process of fluid therapy through rapid adjustment of intravascular volume aiming to improve tissue perfusion. Reversed fluid resuscitation means reducing volume. (4) The right ventricle afterload should be taken into consideration when using stroke volume variation (SVV) or pulse press
Background. In recent years, the identification of individual style regulation of decision-making (DM) has become relevant, since DM is an integral characteristic of human life in a complex and ...uncertain world. As a new aspect, the cross-cultural commonality and specificity of the emotional and personal components of DM are highlighted. Objective. The aim is to carry out a cross-cultural analysis of the decision-making style of Russian and Chinese samples according to productive and unproductive approaches to coping with uncertainty distinguished in the Melbourne Decision Making Questionnaire (MDMQ). Sample. The sample consisted of 531 participants: 259 from Russia (Мage= 27,67; 32,4 % men) and 272 from China (Мage= 27,76; 38,6 % men). Methods. The four-factor structure of MDMQ for the Chinese sample was verified. In addition, the participants were presented with the Positive and Negative Affect Schedule — PANAS. All participants were tested through informed collaboration; in Russian sample it was conducted in person, in Chinese sample online testing through the Wen Zhuan Xing (问卷星) platform was involved. Results. Cross-cultural comparison of sample means revealed greater Vigilance in Russians and greater Back-Passing and Hypervigilance in Chinese participants. Correlation analysis has shown (1) similar interrelations of unproductive coping among Russians and Chinese men and differences in Chinese women; (2) positive relations of Vigilance with positive affect and positive relations of unproductive coping with negative affect. Conclusions. Considering differences in the variables of personal regulation of decision making, we should speak about the generality of their correlation in the latent classes of the two cultural samples.
Preclinical testing is a crucial step in evaluating cancer therapeutics. We aimed to establish a significant resource of patient-derived xenografts (PDXs) of prostate cancer for rapid and systematic ...evaluation of candidate therapies. The PDX collection comprises 59 tumors collected from 30 patients between 2012-2020, coinciding with availability of abiraterone and enzalutamide. The PDXs represent the clinico-pathological and genomic spectrum of prostate cancer, from treatment-naïve primary tumors to castration-resistant metastases. Inter- and intra-tumor heterogeneity in adenocarcinoma and neuroendocrine phenotypes is evident from bulk and single-cell RNA sequencing data. Organoids can be cultured from PDXs, providing further capabilities for preclinical studies. Using a 1 x 1 x 1 design, we rapidly identify tumors with exceptional responses to combination treatments. To govern the distribution of PDXs, we formed the Melbourne Urological Research Alliance (MURAL). This PDX collection is a substantial resource, expanding the capacity to test and prioritize effective treatments for prospective clinical trials in prostate cancer.
1 Research & Development Department, SYM-BIO Life-Science Co. Ltd, Tai-Cang City, Jiang-Su Province 215400, PR China
2 Clinical Laboratory, Nanjing Second Hospital, Nanjing City 210003, PR China
...Correspondence Feng-Bo Wu gaoweiwuli{at}21cn.com
Received 20 December 2007
Accepted 24 March 2008
Current anti-hepatitis C virus (HCV) antibody screening immunoassays are routinely based on an indirect format. Although their use for anti-HCV antibody detection has achieved a very high specificity and sensitivity, false-positive results are still a problem especially among populations with a low prevalence of HCV infection. One strategy to obviate this problem is to adapt the assay from an indirect format to a double-antigen sandwich one to further improve its specificity. In this study, a double-antigen sandwich time-resolved immunofluorometric assay (DAS-TRIFMA) has been developed to detect total anti-HCV antibodies based on biotin–streptavidin interaction. For comparison, 1025 samples were analysed by the DAS-TRIFMA and three indirect anti-HCV antibody detection methods. For samples with discordant results, PCR-ELISA and Inno-LIA were employed as supplementary assays to analyse the presence of HCV antibodies. With regard to the 1025 clinical samples, the overall concordance between the DAS-TRIFMA and the three indirect methods was 99.41, 98.93 and 98.93 % for Ortho ELISA 3.0, WAT ELISA and I-TRIFMA, respectively. The specificity/sensitivity of the DAS-TRIFMA, Ortho HCV ELISA 3.0, WAT HCV ELISA and I-TRIFMA were 100/99.09, 99.34/98.18, 99.23/97.27 and 99.01 %/98.18 %, respectively. The DAS-TRIFMA was able to detect HCV antibodies at a concentration about 1/10 of that detectable by indirect methods. From the obtained results and their comparison, it is concluded that the DAS-TRIFMA is a more specific and reliable method for screening anti-HCV antibodies, and weakly positive S/Co values by the DAS-TRIFMA were more predictive of HCV infection than those by indirect methods.
Abbreviations: DAS-TRIFMA, double-antigen sandwich time-resolved immunofluorometric assay; I-TRIFMA, indirect TRIFMA.
These authors contributed equally to this work.
Rice is the world's most important food crop and a model for cereal research. At 430 megabases in size, its genome is the most compact of the cereals. We report the sequence of chromosome 10, the ...smallest of the 12 rice chromosomes (22.4 megabases), which contains 3471 genes. Chromosome 10 contains considerable heterochromatin with an enrichment of repetitive elements on 10S and an enrichment of expressed genes on 10L. Multiple insertions from organellar genomes were detected. Collinearity was apparent between rice chromosome 10 and sorghum and maize. Comparison between the draft and finished sequence demonstrates the importance of finished sequence.
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