Perovskite solar cells employing CH3NH3PbI3–x Cl x active layers show power conversion efficiency (PCE) as high as 20% in single cells and 13% in large area modules. However, their operational ...stability has often been limited due to degradation of the CH3NH3PbI3–x Cl x active layer. Here, we report a perovskite solar module (PSM, best and av. PCE 10.5 and 8.1%), employing solution-grown TiO2 nanorods (NRs) as the electron transport layer, which showed an increase in performance (∼5%) even after shelf-life investigation for 2500 h. A crucial issue on the module fabrication was the patterning of the TiO2 NRs, which was solved by interfacial engineering during the growth process and using an optimized laser pulse for patterning. A shelf-life comparison with PSMs built on TiO2 nanoparticles (NPs, best and av. PCE 7.9 and 5.5%) of similar thickness and on a compact TiO2 layer (CL, best and av. PCE 5.8 and 4.9%) shows, in contrast to that observed for NR PSMs, that PCE in NPs and CL PSMs dropped by ∼50 and ∼90%, respectively. This is due to the fact that the CH3NH3PbI3–x Cl x active layer shows superior phase stability when incorporated in devices with TiO2 NR scaffolds.
Macrophages derived from monocyte precursors undergo specific polarization processes which are influenced by the local tissue environment: classically activated (M1) macrophages, with a ...pro-inflammatory activity and a role of effector cells in Th1 cellular immune responses, and alternatively activated (M2) macrophages, with anti-inflammatory functions and involved in immunosuppression and tissue repair. At least three different subsets of M2 macrophages, namely, M2a, M2b, and M2c, are characterized in the literature based on their eliciting signals. The activation and polarization of macrophages is achieved through many, often intertwined, signaling pathways. To describe the logical relationships among the genes involved in macrophage polarization, we used a computational modeling methodology, namely, logical (Boolean) modeling of gene regulation. We integrated experimental data and knowledge available in the literature to construct a logical network model for the gene regulation driving macrophage polarization to the M1, M2a, M2b, and M2c phenotypes. Using the software GINsim and BoolNet, we analyzed the network dynamics under different conditions and perturbations to understand how they affect cell polarization. Dynamic simulations of the network model, enacting the most relevant biological conditions, showed coherence with the observed behavior of
macrophages. The model could correctly reproduce the polarization toward the four main phenotypes as well as to several hybrid phenotypes, which are known to be experimentally associated to physiological and pathological conditions. We surmise that shifts among different phenotypes in the model mimic the hypothetical continuum of macrophage polarization, with M1 and M2 being the extremes of an uninterrupted sequence of states. Furthermore, model simulations suggest that anti-inflammatory macrophages are resilient to shift back to the pro-inflammatory phenotype.
Efficient flexible perovskite solar cells and modules were developed using a combination of SnO
2
and mesoporous-TiO
2
as a fully solution-processed electron transport layer (ETL). Cells using such ...ETLs delivered a maximum power conversion efficiency (PCE) of 14.8%, which was 30% higher than the PCE of cells with only SnO
2
as the ETL. The presence of a mesoporous TiO
2
scaffold layer over SnO
2
led to higher rectification ratios, lower series resistances, and higher shunt resistances. The cells were also evaluated under 200 and 400 lx artificial indoor illumination and found to deliver maximum power densities of 9.77 μW/cm
2
(estimated PCE of 12.8%) and 19.2 μW/cm
2
(estimated PCE of 13.3%), respectively, representing the highest values among flexible photovoltaic technologies reported so far. Furthermore, for the first time, a fully laser-patterned flexible perovskite module was fabricated using a complete three-step laser scribing procedure (P1, P2, P3) with a PCE of 8.8% over an active area of 12 cm
2
under an illumination of 1 sun.
As the hole transport layer (HTL) for perovskite solar cells (PSCs), poly(3‐hexylthiophene) (P3HT) has been attracting great interest due to its low‐cost, thermal stability, oxygen impermeability, ...and strong hydrophobicity. In this work, a new doping strategy is developed for P3HT as the HTL in triple‐cation/double‐halide ((FA1−x−yMAxCsy)Pb(I1−xBrx)3) mesoscopic PSCs. Photovoltaic performance and stability of solar cells show remarkable enhancement using a composition of three dopants Li‐TFSI, TBP, and Co(III)‐TFSI reaching power conversion efficiencies of 19.25% on 0.1 cm2 active area, 16.29% on 1 cm2 active area, and 13.3% on a 43 cm2 active area module without using any additional absorber layer or any interlayer at the PSK/P3HT interface. The results illustrate the positive effect of a cobalt dopant on the band structure of perovskite/P3HT interfaces leading to improved hole extraction and a decrease of trap‐assisted recombination. Non‐encapsulated large area devices show promising air stability through keeping more than 80% of initial efficiency after 1500 h in atmospheric conditions (relative humidity ≈ 60%, r.t.), whereas encapsulated devices show more than >500 h at 85 °C thermal stability (>80%) and 100 h stability against continuous light soaking (>90%). The boosted efficiency and the improved stability make P3HT a good candidate for low‐cost large‐scale PSCs.
A new doping strategy is developed for poly(3‐hexylthiophene) (P3HT) as the hole transport layer (HTL) in triple‐cation/double‐halide mesoscopic perovskite solar cells (PSCs), achieving efficiencies of 19.25% and 13.3% on lab‐scale and large‐area module, respectively. Promising stability results after 1500 h air exposure (relative humidity ≈ 60%, r.t.), more than 500 h at 85 °C, and 100 h of continuous light soaking.
Fibro/Adipogenic Progenitors (FAPs) are muscle-interstitial progenitors mediating pro-myogenic signals that are critical for muscle homeostasis and regeneration. In myopathies, the ...autocrine/paracrine constraints controlling FAP adipogenesis are released causing fat infiltrates. Here, by combining pharmacological screening, high-dimensional mass cytometry and in silico network modeling with the integration of single-cell/bulk RNA sequencing data, we highlighted the canonical WNT/GSK/β-catenin signaling as a crucial pathway modulating FAP adipogenesis triggered by insulin signaling. Consistently, pharmacological blockade of GSK3, by the LY2090314 inhibitor, stabilizes β-catenin and represses PPARγ expression abrogating FAP adipogenesis ex vivo while limiting fatty degeneration in vivo. Furthermore, GSK3 inhibition improves the FAP pro-myogenic role by efficiently stimulating, via follistatin secretion, muscle satellite cell (MuSC) differentiation into mature myotubes. Combining, publicly available single-cell RNAseq datasets, we characterize FAPs as the main source of WNT ligands inferring their potential in mediating autocrine/paracrine responses in the muscle niche. Lastly, we identify WNT5a, whose expression is impaired in dystrophic FAPs, as a crucial WNT ligand able to restrain the detrimental adipogenic differentiation drift of these cells through the positive modulation of the β-catenin signaling.
The term micro-heterogeneity refers to non-genetic cell to cell variability observed in a bell-shaped distribution of the expression of a trait within a population. The contribution of ...micro-heterogeneity to physiology and pathology remains largely uncharacterised. To address such an issue, we investigated the impact of heterogeneity in skeletal muscle fibro/adipogenic progenitors (FAPs) isolated from an animal model of Duchenne muscular dystrophy (DMD), the mdx mouse. FAPs play an essential role in muscle homoeostasis. However, in pathological conditions or ageing, they are the source of intramuscular infiltrations of fibrotic or adipose tissue. By applying a multiplex flow cytometry assay, we characterised and purified from mdx muscles two FAP cell states expressing different levels of SCA-1. The two cell states are morphologically identical and repopulate each other after several growth cycles. However, they differ in their in vitro behaviour. Cells expressing higher levels of SCA-1 (SCA1-High-FAPs) differentiate more readily into adipocytes while, when exposed to a fibrogenic stimulation, increase the expression of Col1a1 and Timp1 mRNA. A transcriptomic analysis confirmed the adipogenic propensity of SCA1-High-FAPs. In addition, SCA1-High-FAPs proliferate more extensively ex vivo and display more proliferating cells in dystrophic muscles in comparison to SCA1-Low-FAPs. Adipogenesis of both FAP cell states is inhibited in vitro by leucocytes from young dystrophic mice, while leucocytes isolated from aged dystrophic mice are less effective in limiting the adipogenesis of SCA1-High-FAPs suggesting a differential regulatory effect of the microenvironment on micro-heterogeneity. Our data suggest that FAP micro-heterogeneity is modulated in pathological conditions and that this heterogeneity in turn may impact on the behaviour of interstitial mesenchymal cells in genetic diseases.
In this paper we analyse the electricity consumption of a set of four traditional ‘white goods’ in a panel of ten EU countries observed over the period 1995–2013 with the aim of disentangling the ...amount of technical efficiency from overall energy saving using a stochastic frontier approach. The efficiency trend is modelled as a function of energy efficiency policies and innovation dynamics that combines invention and adoption processes using specific patents weighted by granular production data and worldwide bilateral import flows. Our model also accounts for potential endogeneity arising when innovation processes and economic growth are considered. With this replicable approach, the stochastic frontier framework allows for explicit modelling of innovation processes. Our results show that the efficiency component is related to changes in the energy efficient technological content of appliances. The ‘international’ component represents a predominant share of technological advancement and exerts a significant influence on the transient efficiency. Our evidence calls for an active role to be played by policy makers in focusing on innovation and trade policies in order to achieve more ambitious energy efficiency targets.
•Electricity saving of efficient home appliances is analysed by means of SFA.•Potential endogeneity in the demand frontier function is accounted for.•Technical efficiency is correlated with policy and technology-push mechanisms.•Invention and adoption explain the technical efficiency trend of appliances.•Results stress the importance of supply-push and trade policies to improve EE.
Dye‐sensitized and perovskite solar cells have seen tremendous efforts in their development in recent years. Amongst these developments are the design and implementation of fabrication techniques ...that can guarantee high performance as well as scalability over large areas. Laser processing has become a versatile and important tool in many industries and has also been applied successfully to both types of solar cell technologies, culminating in the demonstration of dye solar devices where all temperature treatments have been replaced with laser techniques, and of high‐performance solid‐state perovskite modules. Herein, we introduce concepts and review the available literature, pertaining to the effective utilization of laser beams for the development of both dye‐sensitized and perovskite photovoltaic technologies.
Frickin’ lasers! Dye‐sensitized and perovskite solar cells have seen tremendous efforts in the design and implementation of fabrication techniques, which can guarantee high performance as well as scalability, precision, and versatility over large areas; prominent amongst these is laser processing. Concepts are introduced and the available literature is reviewed, pertaining to the effective utilization of laser systems applied to these photovoltaic technologies.
Small area hybrid organometal halide perovskite based solar cells reached performances comparable to the multicrystalline silicon wafer cells. However, industrial applications require the scaling-up ...of devices to module-size. Here, we report the first fully laser-processed large area (14.5 cm 2 ) perovskite solar module with an aperture ratio of 95% and a power conversion efficiency of 9.3%. To obtain this result, we carried out thorough analyses and optimization of three laser processing steps required to realize the serial interconnection of various cells. By analyzing the statistics of the fabricated modules, we show that the error committed over the projected interconnection dimensions is sufficiently low to permit even higher aperture ratios without additional efforts.
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