The exceptional Pliocene marine faunal assemblages of west central Portugal have been known since the late 19th century. They include highly diverse molluscan faunas whose study is far to be ...completed. Discovered nearly 40 years ago, Vale do Freixo (Carnide, Pombal) is perhaps the most outstanding fossil site. Neverthless, the bivalves remain relatively unknown. This study focuses on the taxonomy of this relevant group of marine Mollusca. The research, based on a detailed sampling of three fossiliferous beds from the Carnide Formation, yielded a list of 85 species belonging to 75 genera and 32 families. Forty-three species are new for the Carnide area and twenty-three are reported for the first time in the Portuguese Pliocene, increasing to 115 the number of known species in the Mondego Basin in the Beira Litoral.
Some natural scenes of films shooting contain landscapes and other elements that are part of the geological heritage, representing an added value for the growing cultural tourism that visits them. In ...the SE of Burgos province (Spain), near some filming locations of the classic western movie directed by Sergio Leone (1966), “The Good, the Bad and the Ugly”, we have assessed the Geological Heritage of 6 selected sites: (1) Dinosaur tracks; (2) Paleo-river structures preserved; (3) Fossil trees; (4) Sad Hill; (5) Marine Cretaceous fossils; and (6) La Yecla Gorge. Their tourist-cultural, scientific and educational values range from 775 points in La Yecla Gorge site to 450 in Marine Cretaceous fossils site. A virtual tour is presented implementing Augmented Reality on Google Earth with detailed but easily understanding descriptive information of each site, suggested activities for tourism and a free geoapp. The proposed technologies can enhance geotourism, favouring sustainable development and fostering attitudes and skills related to the tourist’s respect for nature.
The municipality of Monsagro, a mountain location in Las Batuecas-Sierra de Francia Natural Park (Salamanca, Spain), has recently developed two paleontological outreach projects: a
Fossil Footprints ...Geo-route
within the urban area and a modern
Centre for the Interpretation of Ancient Seas
. They are based on the abundance and popular interest of
Cruziana
and other ichnofossils, which have been added as architectural elements in many buildings of the village constituting a curious ethnopaleontological phenomenon. The Monsagro ichnofossils are ex situ paleontological heritage submitted to non-standard conditions of conservation. Its scientific value is therefore limited, but its social value is high, and the support of both population and public administration gives the strength for the success of the referred outreach/geotourist initiatives. An on-site survey conducted with village inhabitants and visitors reveals their high popular estimate, and how these have contributed to increasing scientific culture of citizens and socio-economic development of this rural area.
A 3D virtual geological route on Digital Earth of the geological-geomorphological and paleontological heritage in the Algarve (Portugal) is presented, assessing the geological heritage of nine ...representative geosites. Eighteen quantitative parameters are used, weighing the scientific, didactic and cultural tourist interest of each site. A virtual route has been created in Google Earth, with overlaid georeferenced cartographies, as a field guide for students to participate and improve their learning. This free application allows loading thematic georeferenced information that has previously been evaluated by means of a series of parameters for identifying the importance and interest of a geosite (scientific, educational and/or tourist). The virtual route allows travelling from one geosite to another, interacting in real time from portable devices (e.g., smartphone and tablets), and thus making possible the ability to observe the relief and spatial geological distribution with representative images, as well as to access files with the description and analysis of each geosite. By using a field guide, each geosite is complemented with activities for carrying out and evaluating what has been learned; these resources allow a teaching–learning process where the student is an active part of the development and creation of content using new technologies that provide more entertaining and educational learning, teamwork and interaction with social networks. This itinerary allows the creation of attitudes and skills that involve geoconservation as an element for sustainable development.
Following an agronomic approach for the Ca enrichment of Rocha pears, this study aimed to assess the interactions between mineral nutrients in fruit tissues at harvest and after storage for 5 months ...and to characterize the implications on the profile of sugars and fatty acids (FA). A total of seven foliar sprays (with concentrations of 0.1–0.6 kg·ha−1 Ca(NO3)2 and 0.8–8 kg·ha−1 CaCl2) were applied to pear trees. After harvest, the fruits were stored for 5 months, in environmentally controlled chambers, and the mineral contents in five regions (on the equatorial section) of the fruits were assessed, while the sugar and FA content were quantified. For both dates, all foliar sprayed treatments, at different extends, increased Ca content in the center and near the epidermis of Rocha pear fruits and the levels of K, Mn, Fe, Zn and Cu also varied. At harvest, the Ca treatments did not affect the levels of sucrose, glucose, fructose and sorbitol and, after storage, their concentrations remained higher in Ca-treated fruits. Additionally, the tendency of the relative proportions of FA was C18:2 > C18:1 > C16:0 > C18:3 > C18:0 > chains inferior to 16 C (<16:0), but after storage it was C18:2 > C16:0 > C18:3 > C18:0 > C18:1 > chains inferior to 16 C (<16:0). It is concluded that the heterogeneous distribution of Ca in the tissues of Rocha pear fruits results from its absorption in the peel after Ca(NO3)2 and CaCl2 sprays and from the xylemic flux in the core prior to maturity. Additionally, the hydrolysis of complex polysaccharides affects the contents of simpler sugars during maturation, ripening and senescence, while storage decreases the amount of total fatty acids (TFA), but the double bond index (DBI) indicate that cell membrane fluidity remains unaffected.
Calcium is essential for plants, yet as its mobility is limited, the understanding of the rate of Ca2+ accumulation and deposition in tissues of tubers, as well as the interactions with other ...critical nutrients prompted this study. To assess the interactions and differential accumulation of micro and macronutrients in the tissues of tubers, Solanum tuberosum L. varieties Agria and Rossi were cultivated and, after the beginning of tuberization, four foliar sprayings (at 8–10 day intervals) with CaCl2 (3 and 6 kg ha−1) or Ca(NO3)2 (2 and 4 kg ha−1) solutions were performed. It was found that both fertilizers increased Ca accumulation in tubers (mostly in the parenchyma tissues located in the center of the equatorial region). The functioning of the photosynthetic apparatus was not affected until the 3rd application but was somewhat affected when approaching the end of the crop cycle (after the 4th application), although the lower dose of CaCl2 seemed to improve the photochemical use of energy, particularly when compared with the greater dose of Ca(NO3)2. Still, none of these impacts modified tuber height and diameter. Following the increased accumulation of Ca, in the tubers of both varieties, the mean contents of P, K, Na, Fe, and Zn revealed different accumulation patterns. Moreover, accumulation of K, Fe, Mn, and Zn prevailed in the epidermis, displaying a contrasting pattern relative to Ca. Therefore, Ca accumulation revealed a heterogeneous trend in the different regions analyzed, and Ca enrichment of tubers altered the accumulation of other nutrients.
Nowadays, there is a growing concern about micronutrient deficits in food products, with agronomic biofortification being considered a mitigation strategy. In this context, as Zn is essential for ...growth and maintenance of human health, a workflow for the biofortification of grapes from the Vitis vinifera variety Fernão Pires, which contains this nutrient, was carried out considering the soil properties of the vineyard. Additionally, Zn accumulation in the tissues of the grapes and the implications for some quality parameters and on winemaking were assessed. Vines were sprayed three times with ZnO and ZnSO4 at concentrations of 150, 450, and 900 g ha−1 during the production cycle. Physiological data were obtained through chlorophyll a fluorescence data, to access the potential symptoms of toxicity. At harvest, treated grapes revealed significant increases of Zn concentration relative to the control, being more pronounced for ZnO and ZnSO4 in the skin and seeds, respectively. After winemaking, an increase was also found regarding the control (i.e., 1.59-fold with ZnSO4-450 g ha−1). The contents of the sugars and fatty acids, as well as the colorimetric analyses, were also assessed, but significant variations were not found among treatments. In general, Zn biofortification increased with ZnO and ZnSO4, without significantly affecting the physicochemical characteristics of grapes.
One-third of the world’s population is suffering from “hidden hunger” due to micronutrient deficiency. Zinc is acquired through diet, leading its deficiency to the development of disorders such as ...retarded growth, anorexia, infections, and hypogeusia. Accordingly, this study aimed to develop an agronomic workflow for Zn biofortification on two red winegrapes varieties (cv. Castelão and Syrah) and determine the physicochemical implications for winemaking. Both varieties produced in Setúbal (Portugal) were submitted to four foliar applications of ZnSO4 or ZnO (900 and 1350 g ha−1, respectively), during the production cycle. At harvest, Zn biofortification reached a 4.3- and 2.3-fold increase with ZnO 1350 g ha−1 in Castelão and Syrah, respectively (although, with ZnSO4 1350 g ha−1 both varieties revealed an increase in Zn concentration). On a physiological basis, lower values of NDVI were found in the biofortified grapes, although not reflected in photosynthetic parameters with cv. Syrah shows even a potential benefit with the use of Zn fertilizers. Regarding physical and chemical parameters (density, total soluble solids, dry weight, and color), relative to the control no significant changes in both varieties were observed, being suitable for winemaking. It was concluded that ZnSO4 and ZnO foliar fertilization efficiently increased Zn concentration on both varieties without a negative impact on quality, but cv. Castelão showed a better index of Zn biofortification and pointed to a potentially higher quality for winemaking.
Grapes and wine are widely consumed in the world, yet their mineral content can be influenced by many factors such as the mineral composition of soils, viticulture practices and environmental ...conditions. In this context, considering the importance of Zn in the human physiology, the enrichment of Moscatel and Castelão grapes (white and red variety, respectively) with this nutrient prompted this study; further assessment of tissue deposition and some implications for wine production. Using two foliar fertilizers (ZnO or ZnSO4, at 150, 450 and 900 g ha−1), decreases in net photosynthesis and stomatal conductance occurred in both varieties, suggesting that the physiological threshold of Zn toxicity was reached without visible symptoms. Following foliar spraying with both fertilizers, the content of Zn in leaves of the Castelão and Moscatel varieties showed higher values in all treatments relative to the control. Moreover, in grapes this tendency occurred only in Castelão. Concerning Cu, Fe, Ca, K, S and P, some significant differences also happened in leaves and grapes among treatments. At harvest, the indexes of Zn enrichment in grapes increased between 2.14- and 8.38-fold and between 1.02- and 1.44-fold in Castelão and Moscatel varieties, respectively. Zinc in the dried skin of Castelão only increased with ZnO and ZnSO4 sprayed at 900 g ha−1 (ca. 2.71- and 1.5-fold relative to the control, respectively), but in Moscatel a clear accumulation trend could not be found. The dry weight of grapes ranged (in %) between 16 and 23 (but did not vary significantly among treatments of each variety or in each treatment between varieties), and total soluble solids (e.g., mainly soluble sugars and proteins) and color parameters showed some significant variations. Through winemaking, the contents of Zn increased in both varieties (1.34- and 3.57-fold, in Castelão and Moscatel, respectively) and in all treatments, although non-significantly in Castelão. It is concluded that, to increase the contents of Zn in grapes without reaching the threshold of toxicity, ZnO or ZnSO4 can be used for foliar spraying of Castelão and Moscatel varieties until 900 g ha−1 and that winemaking augments the level of this nutrient.
As the productivity and quality of tomato fruits are responsive to Mg applications, without surpassing the threshold of toxicity, the assessment of potential levels of Mg accumulation in tissues, as ...well as the interactions with Ca and physicochemical properties, prompt this study. An agronomic workflow for Mg enrichment, consisting of six foliar applications of MgSO4 with four concentrations (0%, 0.25%, 1% and 4%), equivalent to 0, 43.9, 175.5 and 702 g ha−1, was applied on two tomato (Lycopersicum esculentum L.) genotypes (Heinz1534 and Heinz9205). During fruit development, leaf gas exchange was screened, with only minor physiological deviations being found. At harvest, Mg contents among tissues and the interactions with Ca were analyzed, and it was found that in both varieties a higher Mg/Ca ratio prevailed in the most external part of the fruit sprayed with 4% MgSO4. However, Mg distribution prevailed relatively near the epidermis in H1534, while in H9205 the higher contents of this nutrient occurred in the core of the fruit, which indicated a decrease of the relative proportion of Ca. The morphologic (height and diameter), physical (dry weight and density) and colorimetric parameters, and the total soluble solids of fruits, did not reveal significant changes in both tomato varieties. It was further concluded that foliar application until 4% MgSO4 does not have physiological impacts in the fruit’s quality of both varieties, but in spite of the different patterns of Mg accumulation in tissues, if the mean value in the whole fruit is considered, this nutrient prevails in H1534. This study thus suggests that variety H1534 can be used to attain tomato fruits with added value, providing an option of further processing to achieve food products with functional properties, ultimately proving a beneficial option to producers, the food processing industry and consumers. Moreover, the study reinforces the importance of variety choice when designing enrichment workflows.
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