•Active packaging with Rosmarinus officinalis L. essential oil (REO) is proposed.•The use of REO with meat proved to be efficacious in every storage condition.•High-O2 atmosphere in combination with ...REO were the best conditions for meat.•The shelf life of meat can be extended until day 15.
Rosemary essential oil (REO) contains bioactives having antioxidant and antimicrobial properties. This work investigated the effect of REO combined with modified atmosphere packaging conditions (MAP), in our case, aerobic, vacuum or high O2, to extend the shelf life of beef. Beef slices were wrapped in special three-layer sheets of packaging material, some with a coating of REO (active packaging, AP), and some without REO (non active packaging, NAP), and stored at 4°C for 20days. The use of REO proved efficacious in every storage condition, as seen in the lower counts of psychrotrophics, Brochothrix thermosphacta, Pseudomonas spp., and Enterobacteriaceae in AP meat compared to NAP meat. Sensory and colourimetric analyses showed that the best packaging conditions were high-O2 atmosphere in combination with REO. Based on microbiological data, shelf life of beef was 5–6days for AP samples packaged under aerobic conditions and 14–15days for AP samples in high-O2 conditions.
Application of bioplastics for food packaging Peelman, Nanou; Ragaert, Peter; De Meulenaer, Bruno ...
Trends in food science & technology,
08/2013, Letnik:
32, Številka:
2
Journal Article
Recenzirano
Odprti dostop
This review provides state of the art information on the performance of bioplastics materials, focusing on food packaging. It gives an overview of the main materials used for producing biobased ...films, their limitations, solutions thereof, possible applications and a state of the art on bioplastics already used as a food packaging material.
Furthermore an inventory on bioplastics was made in the context of a research project. Important characteristics regarding packaging material are summarized in an extended table, which shows a big variety (e.g. permeability, tensile properties), suggesting a wide range of food products can be packed in biobased polymer films.
•Overview of biobased polymers used as a component in (food) packaging materials.•Different strategies for improving barrier properties of biobased packaging.•Permeability values and mechanical properties of multilayered biobased plastics.•Wide application area of biobased packaging for food products.
The research objective was to evaluate the performance of biobased plastics for modified atmosphere packaging (MAP) both on laboratory and industrial scales. Therefore the shelf-life (4°C) of ...rumpsteak, ham sausage, filet de saxe, grated cheese and pre-fried fries that were MAP-packed in poly(lactic acid) (PLA) and cellulose-based multilayer packages was evaluated and compared with their shelf-life when packed in conventional materials. Furthermore, tests were performed on industrial packaging lines.
The biobased packages showed sufficient gas-barrier to guarantee the shelf-life of MAP-packed food products, even when materials with lower barrier properties were used, but for rumpsteak and ham sausage, different light permeabilities of the packaging materials led to more discoloration. Furthermore, the biobased materials performed well on the industrial packaging machines, but seemed too brittle to hold larger contents. This study shows promising results toward the application of biobased packaging materials for different food products.
Environmental concerns regarding food packaging have led to the development of more sustainable alternatives. One of these alternatives are biobased materials. This research shows that several biobased plastics can contain and protect the food as well as maintaining its sensory quality. The biobased packages showed sufficient gas barrier to guarantee the shelf-life of the MAP packed food products, even when materials with lower barrier properties were used. Furthermore, case studies at different companies (on industrial packaging lines) show that a successful entrance in the market will not be hindered by technical problems.
•Characteristics of several multilayered biobased plastics•Performance of biobased plastics for MAP packaging of food products (barrier)•Industrial performance (case studies)
The correct labeling of a genetic variant as pathogenic is important as breeding decisions based on incorrect DNA tests can lead to the unwarranted exclusion of animals, potentially compromising the ...long-term health of a population. In human medicine, the American college of Medical Genetics (ACMG) guidelines provide a framework for variant classification. This study aims to apply these guidelines to six genetic variants associated with hypertrophic cardiomyopathy (HCM) in certain cat breeds and to propose a modified criterion for variant classification.
Genetic samples were sourced from five cat breeds: Maine Coon, Sphynx, Ragdoll, Devon Rex, and British Short- and Longhair. Allele frequencies were determined, and in the subset with phenotypes available, odds ratios to determine the association with HCM were calculated.
evaluation followed with joint evidence and data from other publications assisting in the classification of each variant.
Two variants, MYBPC3:c.91G > C A31P and MYBPC3:c.2453C > T R818W, were designated as pathogenic. One variant, MYH7:c.5647G > A E1883K, was found likely pathogenic, while the remaining three were labeled as variants of unknown significance.
Routine genetic testing is advised solely for the MYBPC3:c.91G > C A31P in the Maine Coon and MYBPC3:c.2453C > T R818W in the Ragdoll breed. The human ACMG guidelines serve as a suitable foundational tool to ascertain which variants to include; however, refining them for application in veterinary medicine might be beneficial.
Real-time quantitative PCR can be a very powerful and accurate technique to examine gene transcription patterns in different biological conditions. One of the critical steps in comparing ...transcription profiles is accurate normalisation. In most of the studies published on real-time PCR in horses, normalisation occurred against only one reference gene, usually GAPDH or ACTB, without validation of its expression stability. This might result in unreliable conclusions, because it has been demonstrated that the expression levels of so called "housekeeping genes" may vary considerably in different tissues, cell types or disease stages, particularly in clinical samples associated with malignant disease. The goal of this study was to establish a reliable set of reference genes for studies concerning normal equine skin and equine sarcoids, which are the most common skin tumour in horses.
In the present study the gene transcription levels of 6 commonly used reference genes (ACTB, B2M, HPRT1, UBB, TUBA1 and RPL32) were determined in normal equine skin and in equine sarcoids. After applying the geNorm applet to this set of genes, TUBA1, ACTB and UBB were found to be most stable in normal skin and B2M, ACTB and UBB in equine sarcoids.
Based on these results, TUBA1, ACTB and UBB, respectively B2M, ACTB and UBB can be proposed as reference gene panels for accurate normalisation of quantitative data for normal equine skin, respectively equine sarcoids. When normal skin and equine sarcoids are compared, the use of the geometric mean of UBB, ACTB and B2M can be recommended as a reliable and accurate normalisation factor.
The research aim was to evaluate the applicability
of biobased plastics for packing long shelf-life food
products, both on laboratory and industrial scale. Therefore,
the shelf-life (room ...temperature) of tortilla chips, dry
biscuits and potato flakes packed under air or modified
atmosphere (MAP) in xylan and cellulose-based packages
was evaluated and compared with their shelf-life in reference
(conventional) packaging materials. These tests were
followed by packaging trials on industrial lines. Furthermore,
overall migration studies and printability tests were
performed.
Most of the biobased packages showed sufficient barrier
towards moisture and gasses to serve as a food packaging
material and MAP packaging of long shelf-life food products
is possible. But for very moisture-sensitive food products
(e.g. dry biscuits), no suited packaging material was
found. The quality of the tortilla chips and potato flakes
could be guaranteed during their shelf-life, even if packaging
materials with lower barrier properties were used.
Still, brittleness and seal properties are critical for use on
industrial scale (important for use on vertical flow packaging
machines). Furthermore, the films were printable and
migration tests showed compliance with legislation. This
study shows promising results towards the industrial application
of biobased packaging materials for long shelflife
food products.
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