Waste composting is becoming a key element of integrated waste management. Composting has a number of advantages, including economic benefits, improvement of soil properties through the use of ...compost, reduction in the use of chemical fertilisers, and minimization of environmental pollution. Composting on a landfill surface appears to be an economical solution that can help close the waste loop and material cycle. In this study, a composting plant located on a landfill surface was analysed. The main objective of the research was to identify the species of plants growing in the organic fraction of municipal solid waste in temporary storage, in the composting plant, and in maturing compost located in a reclaimed plot at the landfill site. During monitoring, 88 plant species were identified altogether. It was observed that compost can become a source of weed infestation. To control the presence of weeds in the compost, basic principles of composting are to be followed to reduce the quantity of weed seeds. The thermophilic phase must occur to reduce the viability of seeds in the input materials and sufficient moisture must be ensured during the composting process. When these principles are strictly observed and the stored compost is maintained without vegetation, the supply of seeds in the compost will be low, and the undesirable spread of plant species to adjacent areas will be controlled. The results showed that the use of the obtained compost did not result in the propagation of weed species. This study demonstrates that composting on a reclaimed landfill offers various advantages such as a closed waste management cycle, coverage of the active landfill body, and fertilisation of the reclaimed part of the landfill.
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•Composting on the landfill surfaces can help closing the waste loop and material cycle.•Composts can become sources of weed infestation.•The thermophilic phase must occur to reduce the viability of seeds.•The input materials and moisture must be ensured during the composting process.•The obtained compost did not result in the propagation of weed species.
This observational retrospective study aimed to analyze whether/how the spectrum of bacterial pathogens and their resistance to antibiotics changed during the worst part of the COVID-19 pandemic (1 ...November 2020 to 30 April 2021) among intensive care patients in University Hospital Olomouc, Czech Republic, as compared with the pre-pandemic period (1 November 2018 to 30 April 2019). A total of 789 clinically important bacterial isolates from 189 patients were cultured during the pre-COVID-19 period. The most frequent etiologic agents causing nosocomial infections were strains of Klebsiella pneumoniae (17%), Pseudomonas aeruginosa (11%), Escherichia coli (10%), coagulase-negative staphylococci (9%), Burkholderia multivorans (8%), Enterococcus faecium (6%), Enterococcus faecalis (5%), Proteus mirabilis (5%) and Staphylococcus aureus (5%). Over the comparable COVID-19 period, a total of 1500 bacterial isolates from 372 SARS-CoV-2-positive patients were assessed. While the percentage of etiological agents causing nosocomial infections increased in Enterococcus faecium (from 6% to 19%, p < 0.0001), Klebsiella variicola (from 1% to 6%, p = 0.0004) and Serratia marcescens (from 1% to 8%, p < 0.0001), there were significant decreases in Escherichia coli (from 10% to 3%, p < 0.0001), Proteus mirabilis (from 5% to 2%, p = 0.004) and Staphylococcus aureus (from 5% to 2%, p = 0.004). The study demonstrated that the changes in bacterial resistance to antibiotics are ambiguous. An increase in the frequency of ESBL-positive strains of some species (Serratia marcescens and Enterobacter cloacae) was confirmed; on the other hand, resistance decreased (Escherichia coli, Acinetobacter baumannii) or the proportion of resistant strains remained unchanged over both periods (Klebsiella pneumoniae, Enterococcus faecium). Changes in pathogen distribution and resistance were caused partly due to antibiotic selection pressure (cefotaxime consumption increased significantly in the COVID-19 period), but mainly due to clonal spread of identical bacterial isolates from patient to patient, which was confirmed by the pulse field gel electrophoresis methodology. In addition to the above shown results, the importance of infection prevention and control in healthcare facilities is discussed, not only for dealing with SARS-CoV-2 but also for limiting the spread of bacteria.
This study examined recoveries of silver determination in animal tissues after wet digestion by inductively coupled plasma mass spectrometry. The composition of the mineralization mixture for ...microwave assisted digestion was optimized and the best recoveries were obtained for mineralization with HNO3 and addition of HCl promptly after digestion. The optimization was performed on model samples of chicken meat spiked with silver nanoparticles and a solution of ionic silver. Basic calculations of theoretical distribution of Ag among various silver-containing species were implemented and the results showed that most of the silver is in the form of soluble complexes AgCl2− and AgCl32− for the optimized composition of the mineralization mixture. Three animal tissue certified reference materials were then analyzed to verify the trueness and precision of the results.
•We performed detailed optimization of microwave assisted digestion procedure of animal tissue used prior to Ag determination by ICP-MS.•We provide basic equilibrium calculations to give theoretical explanation of results from optimization of tested mineralization mixtures.•Results from method validation that was done by analysis of several matrix CRMs are presented.
