With the development of micro-LIBS imaging, the ever-increasing size of datasets (sometimes >1 million spectra) makes the processing of spectral data difficult and time consuming. Advanced ...statistical methods have become necessary to process these data, but most of them still require strong expertise and are not adapted to fast data treatment or a high throughput analysis. To address these issues, we evaluate, in the present work, the use of an artificial neural network (ANN) for LIBS imaging spectral data processing for the identification of different mineral phases in archaeological lime mortar. Common in ancient architecture, this building material is a complex mixture of lime with one or more aggregates, some components of which are of the same chemical nature (
e.g.
calcium carbonates). In this study, we trained an artificial neural network (ANN) for automatic detection of different phases in these complex samples. The training of such a predictive model was made possible by building a LIBS dataset of more than 1300 reference spectra, obtained from various selected materials that may be present in mortars. The ANN parameters (pre-treatment of data, number of neurons and of iterations) were optimized to ensure the best recognition of mortar components, while avoiding overtraining. The results demonstrate a fast and accurate identification of each component. The use of an ANN appears to be a strong means to provide an efficient, fast and automated LIBS characterization of archaeological mortar, a concept that could later be generalized to other samples and other scientific fields and methods.
With the development of micro-LIBS imaging, the ever-increasing size of datasets (sometimes >1 million spectra) makes the processing of spectral data difficult and time consuming.
In the ATLAS experiment at the LHC, the output of read-out hardware specific to each subdetector will be transmitted to buffers, located on custom made PCI cards ("ROBINs"). The data consist of ...fragments of events accepted by the first-level trigger at a maximum rate of 100 kHz. Groups of four ROBINs will be hosted in about 150 Read-Out Subsystem (ROS) PCs. Event data are forwarded on request via Gigabit Ethernet links and switches to the second-level trigger or to the Event builder. In this paper a discussion of the functionality and real-time properties of the ROS is combined with a presentation of measurement and modelling results for a testbed with a size of about 20% of the final DAQ system. Experimental results on strategies for optimizing the system performance, such as utilization of different network architectures and network transfer protocols, are presented for the testbed, together with extrapolations to the full system.
The ATLAS collaboration at CERN operated a combined test beam (CTB) from May until November 2004. The prototype of ATLAS data acquisition system (DAQ) was used to integrate other subsystems into a ...common CTB setup. Data were collected synchronously from all the ATLAS detectors, which represented nine different detector technologies. Electronics and software of the first level trigger were used to trigger the setup. Event selection algorithms of the high level trigger were integrated with the system and were tested with real detector data. The possibility to operate a remote Event Filter farm synchronized with the ATLAS Trigger and Data Acquisition System (TDAQ) was also tested. Event data, as well as detector conditions data, were made available for offline analysis
The aim of the present phase I/II study was to evaluate the safety, immune responses and clinical activity of a vaccine based on autologous dendritic cells (DC) loaded with an allogeneic tumor cell ...lysate in advanced melanoma patients. DC derived from monocytes were generated in serum-free medium containing GM-CSF and IL-13 according to Good Manufacturing Practices. Fifteen patients with metastatic melanoma (stage III or IV) received four subcutaneous, intradermal, and intranodal vaccinations of both DC loaded with tumor cell lysate and DC loaded with hepatitis B surface protein (HBs) and/or tetanus toxoid (TT). No grade 3 or 4 adverse events related to the vaccination were observed. Enhanced immunity to the allogeneic tumor cell lysate and to TAA-derived peptides were documented, as well as immune responses to HBs/TT antigens. Four out of nine patients who received the full treatment survived for more than 20 months. Two patients showed signs of clinical response and received 3 additional doses of vaccine: one patient showed regression of in-transit metastases leading to complete remission. Eighteen months later, the patient was still free of disease. The second patient experienced stabilization of lung metastases for approximately 10 months. Overall, our results show that vaccination with DC loaded with an allogeneic melanoma cell lysate was feasible in large-scale and well-tolerated in this group of advanced melanoma patients. Immune responses to tumor-related antigens documented in some treated patients support further investigations to optimize the vaccine formulation.
Chronic kidney disease (CKD) is a world-wide public health problem, with adverse outcomes of kidney failure, cardiovascular disease, and premature death. The National Kidney Foundation, through its ...Kidney Disease Quality Outcome Initiative (K/DOQI) and other National institutions, recommend glomerular filtration rate (GFR) estimates for the definition, classification, screening, and monitoring of CKD. Prediction equations based on serum creatinine values were chosen both for adults (Cockcroft-Gault C-G and Modification of Diet in Renal Disease MDRD study equations) and for children (Schwartz and Counahan-Barratt equations). This review aims to evaluate from recent literature the clinical efficiency and relevance of these equations in terms of bias, precision, and reproducibility in different specific indications (eg, screening CKD, assessment of disease progression, or therapy efficacy) in different populations. Because these prediction equations based on serum creatinine have limitations, especially in the normal or near-normal GFR range, kidney transplant recipients, and pediatric populations, other prediction equations based on serum cystatin C value were also considered as possibly more sensitive GFR surrogate markers. Recent guidelines state that the cystatin C-based prediction equation cannot be recommended for use in clinical practice. With prediction equations based on serum creatinine, the National Kidney Disease Education Program (NKDEP) recommendations are to report a numerical estimate in round numbers only for GFR values <60 mL/min per 1.73 m2 . The MDRD equation generally outperforms the C-G equation but may still have a high level of bias, depending on creatinine assay calibration, and low precision with, at best, approximately 80% of estimated GFR in the “accuracy range” of 70-130% of the measured GFR value, even in patients with known CKD. According to Kidney Disease Improving Global Outcomes (KDIGO) recommendations, many indications remain for GFR measurements using a clearance method. In that context, it should be recalled that radiolabeled-tracer plasma or urinary clearance methods, are safe, simple, accurate and reproducible.
Summary Background Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder associated with respiratory muscle weakness and respiratory failure. Non-invasive ventilation alleviates ...respiratory symptoms and prolongs life, but is a palliative intervention. Slowing the deterioration of diaphragm function before respiratory failure would be desirable. We aimed to assess whether early diaphragm pacing could slow down diaphragm deterioration and would therefore delay the need for non-invasive ventilation. Methods We did a multicentre, randomised, controlled, triple-blind trial in patients with probable or definite ALS in 12 ALS centres in France. The main inclusion criterion was moderate respiratory involvement (forced vital capacity 60–80% predicted). Other key eligibility criteria were age older than 18 years and bilateral responses of the diaphragm to diagnostic phrenic stimulation. All patients were operated laparoscopically and received phrenic stimulators. Clinicians randomly assigned patients (1:1) to receive either active or sham stimulation with a central web-based randomisation system (computer-generated list). Investigators, patients, and an external outcome allocation committee were masked to treatment. The primary outcome was non-invasive ventilation-free survival, analysed in the intention-to-treat population. Safety outcomes were also assessed in the intention-to-treat population. This trial is registered with ClinicalTrials.gov , number NCT01583088. Findings Between Sept 27, 2012, and July 8, 2015, 74 participants were randomly assigned to receive either active (n=37) or sham (n=37) stimulation. On July 16, 2015, an unplanned masked analysis was done after another trial showed excess mortality with diaphragm pacing in patients with hypoventilation (DiPALS, ISRCTN 53817913). In view of this finding, we analysed mortality in our study and found excess mortality (death from any cause) in our active stimulation group. We therefore terminated the study on July, 16, 2015. Median non-invasive ventilation-free survival was 6·0 months (95% CI 3·6–8·7) in the active stimulation group versus 8·8 months (4·2–not reached) in the control (sham stimulation) group (hazard ratio 1·96 95% CI 1·08–3·56, p=0·02). Serious adverse events (mainly capnothorax or pneumothorax, acute respiratory failure, venous thromboembolism, and gastrostomy) were frequent (24 65% patients in the active stimulation group vs 22 59% patients in the control group). No treatment-related death was reported. Interpretation Early diaphragm pacing in patients with ALS and incipient respiratory involvement did not delay non-invasive ventilation and was associated with decreased survival. Diaphragm pacing is not indicated at the early stage of the ALS-related respiratory involvement. Funding Hospital Program for Clinical Research, French Ministry of Health; French Patients' Association for ALS Research (Association pour la Recherche sur la Sclérose Latérale Amyotrophique); and Thierry de Latran Foundation.
St Martin’s church, Angers, is emblematic of the problems raised in pre-12
th
century history of architecture.
In view of the importance of this building, it was necessary to attempt to define its ...dating and this study particularly focuses on its bell-tower. In addition to the conclusion resulting from the interpretation of written sources and typological criteria positioning the construction of the site at the beginning of the 11
th
century, not only a significant number of
14
C dates were carried out on charcoals from the masonry structures, but also independent dating by archaeomagnetism and thermoluminescence were performed on bricks from the bell-tower. The whole results from these three different methods agree and indicate the lower level of the bell-tower was likely built in the 9
th
century, disputing evidence to the theory of construction in the 11
th
century of the church.
Presented here are the detailed results obtained from the thermoluminescence (TL) dating analysis.
Highlights • Compliance with non-invasive ventilation is poor in patients with no subjective symptoms of hypercapnia. • Cessation is more frequent in the case of excessive leaks, ventilator ...dysfunction, or high body mass index. • Cessation is less frequent for patients with a professional occupation.
When properly activated, macrophages can be tumoricidal. To harness the therapeutic potential of these cells, we have developed a process for ex vivo production of large numbers of IFN-
γ-activated ...monocyte-derived macrophages. These monocyte-derived activated killer (MAK
®) cells have been safely administered to cancer patients with minimal residual disease in phase I/II clinical studies. To evaluate efficacy of treatment with MAK cells, phase III clinical studies are necessary. The process of MAK cell production has been further optimized and qualified for use in large cohorts of patients. In this study, we characterized MAK cells produced in large scale by studying their phenotype and functions. MAK cells were shown to exert anti-tumor activity by killing tumor cells and inhibiting their proliferation. These activities were enhanced by activation with IFN-
γ and addition of anti-tumor antibodies. Tumor necrosis factor-alpha (TNF-
α) was one of the mediators used by MAK cells to inhibit tumor proliferation. To facilitate logistics of clinical trials, a process for MAK cell cryopreservation has been developed. We verified in vitro that cryopreserved cells retained the activity of fresh cells and were stable during storage. The safety and efficacy of cryopreserved MAK cells (Bexidem
®) are currently being assessed on superficial bladder cancer patients in a phase II/III clinical trial.
Fibre Channel equipment has been evaluated in the environment of the ATLAS DAQ prototype “−1”. Fibre Channel PCI and PMC cards have been tested on PowerPC-based VME processor boards running LynxOS ...and on Pentium-based personal computers running Windows NT. The performance in terms of overhead and bandwidth has been measured in point-to-point, arbitrated loop and fabric configuration with a Fibre Channel switch. The possible use of the equipment for event building in the ATLAS DAQ prototype “−1” has been studied.
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