Meat plays a significant role in human diets, providing a rich source of high-quality protein. With advancements in technology, research in the field of meat preservation has been undergoing dynamic ...evolution. To gain insights into the development of this discipline, the study conducted an analysis and knowledge structure mapping of 1672 papers related to meat preservation research within the Web of Science Core Collection (WOSCC) spanning from 2001 to 2023. And using software tools such as VOSviewer 1.6.18 and CiteSpace 5.8.R3c allowed for the convenient analysis of the literature by strictly following the software operation manuals. Moreover, the knowledge structure of research in the field of meat preservation was synthesized within the framework of "basic research-technological application-integration of technology with fundamental research," aligning with the research content. Co-cited literature analysis indicated that meat preservation research could be further categorized into seven collections, as well as highlighting the prominent role of the antibacterial and antioxidant properties of plant essential oils in ongoing research. Subsequently, the future research direction and focus of the meat preservation field were predicted and prospected. The findings of this study could offer valuable assistance to researchers in swiftly comprehending the discipline's development and identifying prominent research areas, thus providing valuable guidance for shaping research topics.
In response to muscle injury, muscle stem cells are stimulated by environmental signals to integrate into damaged tissue to mediate regeneration. L-leucine (L-leu), a branched-chain amino acid (BCAA) ...that belongs to the essential amino acids (AAs) of the animal, has gained global interest on account of its muscle-building and regenerating effects. The present study was designed to investigate the impact of L-leu exposure to promote the proliferation of equine skeletal muscle satellite cells (SCs) on the regulation of RNA networks, including mRNA, long non-coding RNA (lncRNA), covalently closed circular RNA (circRNA), and microRNA (miRNA) in skeletal muscles. Equine SCs were used as a cell model and cultured in different concentrations of L-leu medium. The cell proliferation assay found that the optimal concentration of L-leu was 2 mM, so we selected cells cultured with L-leu concentrations of 0 mM and 2 mM for whole-transcriptiome sequencing, respectively. By high-throughput sequencing analysis, 2470 differentially expressed mRNAs (dif-mRNAs), 363 differentially expressed lncRNAs (dif-lncRNAs), 634 differentially expressed circRNAs (dif-circRNAs), and 49 differentially expressed miRNAs (dif-miRNAs) were significantly altered in equine SCs treated with L-leu. To identify the function of autoimmunity and anti-inflammatory responses after L-leu exposure, enrichment analysis was conducted on those differentially expressed genes (DEGs) related to lncRNA, circRNA, and miRNA. The hub genes were selected from PPI Network, including ACACB, HMGCR, IDI1, HAO1, SHMT2, PSPH, PSAT1, ASS1, PHGDH, MTHFD2, and DPYD, and were further identified as candidate biomarkers to regulate the L-leu-induced proliferation of equine SCs. The up-regulated novel 699_star, down-regulated novel 170_star, and novel 360_mature were significantly involved in the competing endogenous RNA (ceRNA) complex network. The hub genes involved in cell metabolism and dif-miRNAs may play fundamental roles in the L-leu-induced proliferation of equine SCs. Our findings suggested that the potential network regulation of miRNAs, circ-RNAs, lncRNAs, and mRNAs plays an important role in the proliferation of equine SCs, so as to build up new perspectives on improving equine performance and treatment strategies for the muscle injuries of horses.
Microorganisms residing in the cecum of donkeys are crucial for physiological processes, nutrient metabolism, and immune function. Feeding methods can affect the dynamic balance of animal gut ...microbiota, thereby affecting indicators such as volatile fatty acids. This study explores suitable feeding methods to promote actual production by changing the feeding order of concentrate. Fifteen Dezhou donkeys with similar age and weight profiles were randomly divided into three groups with the concentrate feeding sequence: fiber-to-concentrate (FC), concentrate-to-fiber ( CF ), and total mixed ration (TMR). The experiment spanned a duration of 82 days. The analyses conducted were primarily aimed at determining the effects of feeding on gut microbes, primarily using metagenomic sequencing techniques. The experimental findings revealed that the levels of valeric acid were notably higher in the CF and TMR groups compared to the FC group ( p < 0.05). These results suggest that the feeding sequence exerts a certain impact on the microbial composition within the cecum of Dezhou donkeys. At the phylum level, the predominant microbiota consisted of Firmicutes and Bacteroidetes, with the CF group displaying a higher relative abundance of Firmicutes compared to both the FC and TMR groups. At the genus level, Prevotella , Bacteroides , and Fibrobacter were the dominant bacterial genera identified in cecum. The functional gene annotation analysis indicated a significantly lower abundance of lacZ (K01190), Por/nifJ (K03737), and ppdK (K01006) genes in CF group relative to the FC and TMR groups ( p < 0.05), highlighting their roles in galactose metabolism and glycolysis, respectively. Moreover, the CF group exhibited a higher concentration of antibiotic resistance genes ( tetO and tet44 ) in the gut microbiota compared to the TMR and FC groups ( p < 0.05), underscoring the presence of numerous antibiotic resistance genes within the phyla Bacteroidetes, Firmicutes, and Proteobacteria. In conclusion, different precision feed sequences significantly impact the levels of volatile fatty acids in Dezhou fattening donkeys, modify the composition and functional genes of the cecal microbiota, and elucidate the microbial mechanisms influenced by the feeding sequence on the growth and metabolism. These insights are anticipated to provide a foundation for the rational design of precision feed sequences in practical agricultural settings.
Cobalt carbide (Co2C) nanoprisms derived from CoMn composite oxides exhibit promising catalytic performance for Fischer–Tropsch to olefins (FTO) synthesis via H2-lean syngas conversion, but with ...nearly 45 C% of CO2 selectivity. The work herein was aimed to investigate the effect of CO2 in the feed on the structure–performance relationship of Co2C-based catalysts during a realistic FTO process. An obvious negative effect of CO2 was observed on the catalytic performance, and the presence of CO2 greatly decreased the catalytic activity and olefin formation rate, while it facilitated methane formation. In addition, the product distribution shifted toward light components at increasing CO2 content, and a typical methanation regime with low selectivity to olefins was observed for CO2 hydrogenation. A structural characterization suggested that the Na-promoted Co2C nanoprisms remained stable under FTO working conditions, and weak linearly and bridge adsorbed CO molecules were observed when the temperature reached 250 °C in a flow of CO-containing gas. However, the CO2 environment hindered CO adsorption, and the strong CO2 adsorption ability led to decreased CO coverage and a high local H2/CO ratio on the catalyst surface. The as-obtained CO-lean and H-rich surface microenvironment gradually changed the morphology of Co2C nanostructures from nanoprisms to nanospheres. Some of the Co2C was even transformed into metallic Co. The change of the catalyst structure and the surrounding environment inhibited the adsorption of surface intermediates and the subsequent chain growth. This work provides important insights for further catalyst optimization and suggests that CO2 removal is necessary for recycling the tail gas or using CO2-containing feedstocks for industrial FTO processes over Co2C-based catalysts.
A multifunctional catalyst composed of CoMn and CuZnAlZr oxides can dramatically increase higher-alcohol selectivity. However, the role of Cu-based components and catalytic mechanism are still ...unclear. Herein, a series of multifunctional catalysts containing CoMn oxides and different Cu-based components were constructed to investigate the influence of a Cu component, and a link between methanol synthesis and higher-alcohol synthesis was established. It was found that the Cu-based components with different methanol synthesis activity showed a different promotional effect for higher-alcohol synthesis. The sole Cu-based component exhibited higher activity for methanol synthesis, and higher selectivity for C2+ oxygenates was achieved for the corresponding mixed catalyst system. In situ DRIFTs indicated that the CH x O* species were mainly produced over the Cu-based components. The formed CH x O* species bridged the CoMn and Cu-based component and promoted the formation of higher alcohols.
•The deactivation process of CuCo/TiO2 model catalyst for HAS was studied.•The changes of catalytic performance and structure evolution were investigated.•Catalytic activity decreased with ...time-on-stream.•The products shifted to low carbon number with time-on-stream.•Sintering and CoxC formation led to catalytic deactivation.
Changes in catalytic performance and catalyst structural evolution during the deactivation of the CuCo/TiO2 model catalyst for higher alcohol synthesis via syngas were investigated. The catalytic activity decreases continuously with time-on-stream, although there is no obvious change in total alcohol selectivity. Meanwhile, the products shift gradually to low carbon number, and the chain growth probabilities of both alcohols and hydrocarbons decrease with reaction time. Using ethane hydrogenolysis as the probe reaction, the catalytic activity also declines with time-on-stream, indicating the decrease in the number of surface metallic cobalt ensembles. To elucidate the structural evolution of the catalyst, the calcined, reduced, and used catalysts were analyzed by various techniques. The bulk crystalline phase of the calcined catalyst is CuCo composite oxide, with small CuO nanoparticles spreading on the catalyst surface. After reduction, the oxides are transformed into CuCo alloy and Cu nanoparticles. For the used sample, severe sintering occurs and CoxC forms on the catalyst surface, both of which reduce the number of surface Co atoms and leads to catalytic deactivation.
Selective synthesis of higher oxygenates from syngas provides a promising route for the conversion of nonpetroleum carbon resources into valuable chemicals. However, it remains a grand challenge to ...design highly efficient and stable dual-sites structures to promote the production of higher oxygenates. Herein, we reported an effective method to maximize the interface of dual active sites via designing the structure of alloy carbide derived from the FeCo layered double hydroxide precursor. Cobalt atoms were well-distributed and doped into Fe2C to form (Fe x Co y )2C alloy carbide. The atomic-scale contact Fe–Co interfacial sites could achieve a >35% oxygenate selectivity at a CO conversion of >80% during 200 h of running, and a high space–time yield of 183.9 mg/gcat./h for oxygenates with 95.6% being the C2+OH fraction was obtained. The kinetic study confirmed that the apparent activation energy of (Fe x Co y )2C alloy carbide was lower than that of separated Fe2C-Co2C dual sites. This work provides a strategy for the design of an effective catalyst for selective synthesis of higher oxygenates from syngas by tuning the interface of dual active sites at an atomic level.
Constructing highly efficient dual active sites for preferential formation of higher oxygenates via direct syngas conversion remains a grand challenge. Herein, we reported that the regulation of ...oxygen vacancy density of metal–oxide support could effectively promote the production of oxygenates. Compared with an inert SiO2-supported Co-based catalyst, the rutile TiO2-supported catalyst with abundant oxygen vacancies exhibited up to 44.7% CO conversion with the selectivity and space–time yield (STY) of the oxygenate increased to 43.4 wt % and 50 mg gcat. –1 h–1, respectively. Further studies established a nearly linear relationship between the density of the oxygen vacancy and the atomic ratio of Co2+/Co0 or the STY of oxygenated products. Characterization confirmed that the oxygen vacancies not only promote CO adsorption, dissociation, and subsequently the carburization of cobalt species to form Co2C but also create a C-rich and H-poor local microchemical environment that benefits CO associative adsorption and CO bond insertion to form oxygenates. The synergistic effect of oxygen vacancies and the Co0/Co2C interface site contributed to the observed enhanced performance for direct syngas conversion to higher oxygenates.
The promotion effect of manganese on the Ru-based Fischer–Tropsch to olefins (FTO) reaction was investigated. Methane formation and second hydrogenation of olefins were effectively suppressed, while ...catalytic activity and olefin formation, especially for long-chain olefins, were greatly promoted with the addition of Mn. Structure characterization suggested that the addition of Mn could promote the dispersion of Ru nanoparticles (NPs) and enhance the electron density of metallic Ru sites, thus boosting the level of CO adsorption and dissociation. The electronic effect of Mn also creates a C*-rich local chemical environment that could weaken the hydrogenation of olefins. This work provides a simple and feasible way to improve Ru atom utilization for the rational design of Ru-based FTO catalysts.
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