Two groups of monoclonal antibodies (MAbs) specific to human cardiac troponin I (cTnI) were generated by immunization of mice by isolated cTnI (group I, 16 MAbs) or by the whole troponin complex ...(group II, 15 MAbs). Two sets of overlapping decapeptides covering the complete sequence of cTnI were prepared and used for epitope mapping by SPOT technique. Majority of MAbs (28 out of 31) interacts with synthetic peptides thus indicating that they recognize liner epitopes. MAbs raised against isolated cTnI preferentially recognize epitopes located at the N- or C-terminal ends of cTnI. Nine out of fifteen MAbs raised against whole troponin complex interact with epitopes located in the N-terminal part of cTnI. Generation of MAbs recognizing both isolated cTnI and cTnI inside of troponin complex and mapping their epitopes provides reliable detection of TnI in serum of patients with acute myocardial infarction.
Performances of a pneumatically actuated micropump are described. Simulation results combined to an analytical model are used to optimize the micropump design in order to obtain a working frequency ...of 10 Hz for pneumatic pressure varying between 0.1-6 bar. A pneumatic chamber is placed between a polymer membrane to be actuated, and microchannels connected to a vacuum system. Time for pressurizing cycle and vacuum cycle as function of design parameters and relative pressure are calculated.
We investigate instantons on sine-cones over Sasaki-Einstein and 3-Sasakian manifolds. It is shown that these conical Einstein manifolds are K"ahler with torsion (KT) manifolds admitting Hermitian ...connections with totally antisymmetric torsion. Furthermore, a deformation of the metric on the sine-cone over 3-Sasakian manifolds allows one to introduce a hyper-K"ahler with torsion (HKT) structure. In the large-volume limit these KT and HKT spaces become Calabi-Yau and hyper-K"ahler conifolds, respectively. We construct gauge connections on complex vector bundles over conical KT and HKT manifolds which solve the instanton equations for Yang-Mills fields in higher dimensions.
PDF
